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Bioeng. Biotechnol."],"abstract":"<jats:p>Nowadays, it is still quite difficult to combat glioblastoma, which is one of the most lethal cancers for human beings. Combinatory therapy, which could not only improve therapeutic efficacy and overcome multiple drug resistance but also decrease the threshold therapeutic drug dosage and minimize side effects, would be an appealing candidate for glioblastoma treatment. Herein, we report fluorescence imaging in the second near-infrared window (NIR-II)-guided combinatory photothermal therapy (PTT) and chemotherapy of glioblastoma with a newly formulated nanomedicine termed <jats:bold>PATSL<\/jats:bold>. It is composed of temperature-sensitive liposome (TSL) carriers, NIR-II emissive and photothermal aggregation-induced emission (AIE) dyes, and chemotherapeutic paclitaxel (PTX) as well. <jats:bold>PATSL<\/jats:bold> shows spherical morphology with diameters of approximately 55 and 85\u00a0nm by transmission electron microscopy and laser light scattering, respectively, a zeta potential of \u221214.83 mV, good stability in both size and photoactivity, strong light absorption with a peak of approximately 770\u00a0nm, and bright emission from 900\u00a0nm to 1,200\u00a0nm. After excitation with an 808-nm laser with good spatiotemporal controllability, <jats:bold>PATSL<\/jats:bold> emits bright NIR-II fluorescence signals for tumor diagnosis <jats:italic>in vivo<\/jats:italic>, exhibits high photothermal conversion efficiency (68.8%), and triggers drug release of PTX under hypothermia, which assists in efficient tumor ablation <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. This research demonstrates that \u201call-in-one\u201d theranostics with NIR-II fluorescence imaging-guided combinatory PTT and chemotherapy is an efficient treatment paradigm for improving the prognosis of brain cancers.<\/jats:p>","DOI":"10.3389\/fbioe.2023.1343694","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T10:06:36Z","timestamp":1703757996000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Light-activatable and hyperthermia-sensitive \u201call-in-one\u201d theranostics: NIR-II fluorescence imaging and chemo-photothermal therapy of subcutaneous glioblastoma by temperature-sensitive liposome-containing AIEgens and paclitaxel"],"prefix":"10.3389","volume":"11","author":[{"given":"Lixin","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menglong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubham","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghe","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,12,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1016\/j.jconrel.2020.02.020","article-title":"Recent strategies and advances in the fabrication of nano lipid carriers and their application towards brain targeting","volume":"321","author":"Agrawala","year":"2020","journal-title":"J. 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