{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:48:11Z","timestamp":1753890491450,"version":"3.41.2"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T00:00:00Z","timestamp":1745539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Inteins can connect flanking external proteins into a new protein fragment and excise themselves. Here, we report the <jats:italic>in situ<\/jats:italic> splicing of proteins by engineered microbial consortia. This study pioneers a programmable microbial consortia platform enabling <jats:italic>in situ<\/jats:italic> protein splicing through split intein-mediated assembly. Engineered <jats:italic>Escherichia coli<\/jats:italic> with the ePop autolysis system release intein-fused protein fragments via synchronized lysis, while <jats:italic>Pichia pastoris<\/jats:italic> secretes complementary domains, enabling extracellular reconstitution directly in culture. With the application of integrating quorum-sensing controls and eukaryotic secretion pathways, this approach bypasses <jats:italic>in vitro<\/jats:italic> purification, supporting scalable one-pot synthesis of multiple functional proteins. The platform\u2019s versatility in logic computation and antibiotic resistance engineering highlights its potential for adaptive biomanufacturing and context-aware biomaterial design.<\/jats:p>","DOI":"10.3389\/fbioe.2025.1529655","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T05:26:29Z","timestamp":1745558789000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["In situ intein-mediated multiprotein assembly via engineered cross-species consortia"],"prefix":"10.3389","volume":"13","author":[{"given":"Hao","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajia","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"19411","DOI":"10.1038\/s41598-021-98855-3","article-title":"Production of IgG1-based bispecific antibody without extra cysteine residue via intein-mediated protein trans-splicing","volume":"11","author":"Akiba","year":"2021","journal-title":"Sci. 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