{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T03:12:57Z","timestamp":1789528377398,"version":"build-2803163510"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Bacterial resistance remains a significant challenge, necessitating the development of new antibacterial strategies. This study introduces a rapidly synthesized L\u2010histidine\u2010 Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> (L\u2010His\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>) nanozyme with enhanced peroxidase (POD)\u2010like activity, designed to improve antibacterial efficacy and accelerate the healing of bacteria-infected wounds.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> We successfully synthesized L\u2010His\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> using an ultrafast, room-temperature synthesis method, and observed its anti-infection effect and explored its anti-infection mechanism through <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> antibacterial experiments.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> We produced L\u2010His\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> cost-effectively while preserving L\u2010His, which was essential for its catalytic and antibacterial functions. The resulting nanozyme demonstrated exceptional antibacterial activity against both Gram-positive (<jats:italic>S. aureus<\/jats:italic>) and Gram-negative (<jats:italic>E. coli<\/jats:italic>) bacteria. <jats:italic>In vivo<\/jats:italic> experiments revealed that L\u2010His\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> outperformed vancomycin in reducing bacterial viability and effectively promoting wound healing, all while maintaining excellent biosafety with no adverse effects on blood or liver functions.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> These findings highlight the potential of L\u2010His\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> for large-scale production and practical use in treating bacterial infections, offering a promising approach to combating antibiotic-resistant pathogens.<\/jats:p>","DOI":"10.3389\/fbioe.2025.1548025","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T07:07:16Z","timestamp":1743145636000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Ultrafast synthesis of L-His-Fe3O4 nanozymes with enhanced peroxidase-like activity for effective antibacterial applications"],"prefix":"10.3389","volume":"13","author":[{"given":"Ye","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huidan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lantian","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2502","DOI":"10.1056\/NEJMc1514294","article-title":"Transient smartphone blindness","volume":"374","author":"Alim-Marvasti","year":"2016","journal-title":"N. 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