{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:50:57Z","timestamp":1790034657670,"version":"4.0.1"},"reference-count":70,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec><jats:title>Background\/objectives<\/jats:title><jats:p>The growing demand for reliable and stable biocatalysts has spurred research into microbial lipases for diverse industrial applications. This study focused on enhancing the production and purification of a lipase from <jats:italic>Streptomyces gobitricini<\/jats:italic> (Lip<jats:sub>S.g<\/jats:sub>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Maximal lipase activity (420\u00a0U\/mL) was achieved during the stationary phase after 84\u00a0h of incubation at 45\u00b0C and pH 8.0, using 2% glucose and 2% yeast extract as carbon and nitrogen sources, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Calcium, olive oil, and Tween, at 1%, significantly enhanced Lip<jats:sub>S.g<\/jats:sub> production, highlighting the role of triglycerides and detergents in enzyme induction and substrate emulsification. The purified 50-kDa enzyme displayed maximal activity at 50\u00b0C and pH 9.0, with thermal stability between 40\u00b0C and 55\u00b0C and pH 5.0\u201310.0. While Lip<jats:sub>S.g<\/jats:sub> efficiently hydrolyzed short and medium-chain triglycerides, it exhibited a preference for long-chain substrates, with a maximum reaction rate of 2500\u00a0\u03bcmol\/min\/mg and a K<jats:sub>m<\/jats:sub> value of 6.45\u00a0mM toward triolein (C18). Lip<jats:sub>S.g<\/jats:sub> also demonstrated remarkable stability in detergent formulations, retaining more than 85% activity in the presence of surfactants, oxidizing agents, boron compounds, and enzyme inhibitors. Additionally, Lip<jats:sub>S.g<\/jats:sub> catalyzed the esterification of oleic acid with starch and ethanol to produce starch oleate and ricinoleic acid.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings establish Lip<jats:sub>S.g<\/jats:sub> as a promising biocatalyst for applications in biocatalysis and detergent formulations, with potential uses in the food, beverage, cosmetic, and pharmaceutical industries.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fbioe.2025.1589087","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T05:22:12Z","timestamp":1747372932000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Versatile biocatalyst: lipase from Streptomyces gobitricini for ester synthesis and detergent innovation"],"prefix":"10.3389","volume":"13","author":[{"given":"Areej Ali","family":"Alzahrani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najeh","family":"Krayem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Alonazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihan M.","family":"Al-Ghamdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Horchani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abir","family":"Ben Bacha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s12934-025-02664-6","article-title":"Microbial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine","volume":"24","author":"Abdelaziz","year":"2025","journal-title":"Microb. 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