{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:28:42Z","timestamp":1791804522554,"version":"4.3.4"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Infected bone defects represent one of the most prevalent clinical conditions, affecting millions of patients annually. The local infection and necrosis associated with these defects exacerbate the injury, prolong healing times, and result in significant localized pain, presenting a substantial challenge for clinical repair. In this study, we developed a biomimetic mineralized and antibacterial imCOL1MA hydrogel by employing methacrylated COL1, composite native bone inorganic salts (CNBIS), and Magainin II-PLGA microspheres (mMicrospheres), which was further loaded with bone marrow stem cells (BMSCs) to form osteogenic engineered bone for infected bone defects repair. Briefly, we first optimized the concentration of COL1MA for BMSCs survival, then adjusted proportion of CNBIS to create an appropriate osteoinductive microenvironment, and encapsulated Magainin II in poly (lactic-co-glycolic acid) (PLGA) microsphere for long-term antimicrobial function. Consequently, the promising mineralized and antibacterial imCOL1MA was prepared using 10% COL1MA, 2% CNBIS, and 1% mMicrospheres. The imCOL1MA scaffold served as significant antimicrobial efficacy, excellent biodegradability, good biocompatibility, and osteoinductive microenvironment. As a result, the engineered bone could achieve rapid (only 4\u00a0weeks) vascularized and neuralized bone regeneration in a rabbit model of infected bone defects.<\/jats:p>","DOI":"10.3389\/fbioe.2025.1611639","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T05:26:40Z","timestamp":1747805200000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid neuralized and vascularized osteogenesis in infected bone defect using biomimetic biomineralized and antibacterial hydrogels"],"prefix":"10.3389","volume":"13","author":[{"given":"Yuhao","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maimaitiaili","family":"Tuerxun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuekang","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfei","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2305580","DOI":"10.1002\/advs.202305580","article-title":"Tolerant and rapid endochondral bone regeneration using framework\u2010enhanced 3D biomineralized matrix hydrogels","volume":"11","author":"Bai","year":"2024","journal-title":"Adv. Sci."},{"key":"B2","doi-asserted-by":"publisher","first-page":"7023","DOI":"10.1038\/s41467-024-51211-1","article-title":"Piezo1 expression in neutrophils regulates shear-induced NETosis","volume":"15","author":"Baratchi","year":"2024","journal-title":"Nat. Commun."},{"key":"B3","doi-asserted-by":"publisher","first-page":"025010","DOI":"10.1088\/1758-5090\/adaf59","article-title":"Collagen silver-doped hydroxyapatite scaffolds reinforced with 3D printed frameworks for infection prevention and enhanced repair of load-bearing bone defects","volume":"17","author":"Genoud","year":"2025","journal-title":"Biofabrication"},{"key":"B4","doi-asserted-by":"publisher","first-page":"031103","DOI":"10.1063\/5.0257593","article-title":"Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts","volume":"13","author":"Hou","year":"2025","journal-title":"Apl. Mater."},{"key":"B5","doi-asserted-by":"publisher","first-page":"e2403817","DOI":"10.1002\/adhm.202403817","article-title":"Ginsenoside rd\u2010loaded antioxidant polymersomes to regulate mitochondrial homeostasis for bone defect healing in periodontitis","volume":"14","author":"Hu","year":"2025","journal-title":"Adv. Healthc. Mater."},{"key":"B6","doi-asserted-by":"publisher","first-page":"460","DOI":"10.7150\/thno.103725","article-title":"Advancements in GelMA bioactive hydrogels: strategies for infection control and bone tissue regeneration","volume":"15","author":"Huang","year":"2025","journal-title":"Theranostics"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10856-024-06852-5","article-title":"True-bone-ceramics\/type I collagen scaffolds for repairing osteochondral defect","volume":"36","author":"Jiang","year":"2024","journal-title":"J. Mater Sci. Mater Med."},{"key":"B8","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/s41205-025-00255-0","article-title":"Trabecular-bone mimicking osteoconductive collagen scaffolds: an optimized 3D printing approach using freeform reversible embedding of suspended hydrogels","volume":"11","author":"Kontakis","year":"2025","journal-title":"3D Print Med."},{"key":"B9","doi-asserted-by":"publisher","first-page":"20891","DOI":"10.1021\/jacs.4c05377","article-title":"Antimicrobial peptides increase line tension in raft-forming lipid membranes","volume":"146","author":"Koynarev","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"B10","doi-asserted-by":"publisher","first-page":"6858","DOI":"10.1021\/acsnano.4c12343","article-title":"Multienzyme-activity sulfur quantum dot nanozyme-mediated cascade reactions in whole-stage symptomatic therapy of infected bone defects","volume":"19","author":"Liu","year":"2025","journal-title":"ACS Nano"},{"key":"B11","doi-asserted-by":"publisher","first-page":"123455","DOI":"10.1016\/j.carbpol.2025.123455","article-title":"Recent progress in nanocellulose-based biocomposites for bone tissue engineering and wound healing applications","volume":"357","author":"Parthasarathy","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"B12","volume-title":"A soft, ultra-tough and multifunctional artificial muscle for volumetric muscle loss treatment","author":"Peng-Fei","year":"2025"},{"key":"B13","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1007\/s00701-024-06282-w","article-title":"Complication rates after autologous cranioplasty following decompressive craniectomy","volume":"166","author":"Ritter","year":"2024","journal-title":"Acta Neurochir."},{"key":"B14","doi-asserted-by":"publisher","first-page":"214170","DOI":"10.1016\/j.bioadv.2024.214170","article-title":"Biofunctionalisation of porous additively manufactured magnesium-based alloys for Orthopaedic applications: a review","volume":"169","author":"Sadat Hashemi","year":"2025","journal-title":"Biomater. Adv."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1502","DOI":"10.1038\/s41598-024-79536-3","article-title":"Finite element investigation for improving chest wall reconstruction process using ceramic and polymeric implants","volume":"15","author":"Shash","year":"2025","journal-title":"Sci. Rep."},{"key":"B16","doi-asserted-by":"publisher","first-page":"218","DOI":"10.3390\/biomimetics9040218","article-title":"Nanocomposite methacrylated silk fibroin-based scaffolds for bone tissue engineering","volume":"9","author":"Spessot","year":"2024","journal-title":"Biomimetics"},{"key":"B17","doi-asserted-by":"publisher","first-page":"2404900","DOI":"10.1002\/smll.202404900","article-title":"Near\u2010infrared carbon dots with antibacterial and osteogenic activities for sonodynamic therapy of infected bone defects","volume":"20","author":"Tang","year":"2024","journal-title":"Small"},{"key":"B18","doi-asserted-by":"publisher","first-page":"e944713","DOI":"10.12659\/MSM.944713","article-title":"Comparative analysis of gelatin\/polylactic acid and commercial pla membranes for guided bone regeneration: a randomized clinical trial","volume":"30","author":"Wang","year":"2024","journal-title":"Med. Sci. Monit."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2416715","DOI":"10.1002\/adma.202416715","article-title":"Osteomimix: a multidimensional biomimetic cascade strategy for bone defect repair","volume":"37","author":"Wu","year":"2025","journal-title":"Adv. Mater."},{"key":"B20","doi-asserted-by":"publisher","first-page":"2409683","DOI":"10.1002\/advs.202409683","article-title":"Programmed transformation of osteogenesis microenvironment by a multifunctional hydrogel to enhance repair of infectious bone defects","volume":"12","author":"Xie","year":"2025","journal-title":"Adv. Sci."},{"key":"B21","doi-asserted-by":"publisher","first-page":"2402174","DOI":"10.1002\/smtd.202402174","article-title":"Ultrasound activated piezoelectric catalysis and neurogenic activity for effective therapy of MRSA infected bone defects by phase\/defect\u2010engineered barium strontium titanate","author":"Xu","year":"2025","journal-title":"Small Methods"},{"key":"B22","doi-asserted-by":"publisher","first-page":"2500092","DOI":"10.1002\/adhm.202500092","article-title":"Nanohybrid hydrogel with dual functions: controlled low\u2010temperature photothermal antibacterial activity and promoted regeneration for treating MRSA\u2010infected bone defects","volume":"14","author":"Yang","year":"","journal-title":"Adv. Healthc. Mater."},{"key":"B23","doi-asserted-by":"publisher","first-page":"2407730","DOI":"10.1002\/adma.202407730","article-title":"Directional mushroom\u2010derived scaffold for microenvironment regulation in infected bone defects","volume":"37","author":"Yang","year":"","journal-title":"Adv. Mater."},{"key":"B24","doi-asserted-by":"publisher","first-page":"101481","DOI":"10.1016\/j.mtbio.2025.101481","article-title":"pH-responsive molybdenum disulphide composite nanomaterials for skin wound healing using \u201cROS leveraging\u201d synergistic immunomodulation","volume":"31","author":"Yang","year":"","journal-title":"Mater. Today Bio"},{"key":"B25","doi-asserted-by":"publisher","first-page":"396","DOI":"10.3390\/biom15030396","article-title":"Extracellular vesicles from adipose-derived mesenchymal stem cells improve ligament\u2013bone integration after anterior cruciate ligament primary repair in rabbit","volume":"15","author":"Yanuar","year":"2025","journal-title":"Biomolecules"},{"key":"B26","doi-asserted-by":"publisher","first-page":"101550","DOI":"10.1016\/j.mtbio.2025.101550","article-title":"Dual functional hydrogel of osteoclastic-inhibition and osteogenic-stimulation for osteoporotic bone defect regeneration","volume":"31","author":"Yu","year":"2025","journal-title":"Mater. Today Bio"},{"key":"B27","doi-asserted-by":"publisher","first-page":"5027","DOI":"10.1038\/s41467-024-49280-3","article-title":"Synthetic biodegradable microporous hydrogels for in vitro 3D culture of functional human bone cell networks","volume":"15","author":"Zauchner","year":"2024","journal-title":"Nat. Commun."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1563546","DOI":"10.3389\/fbioe.2025.1563546","article-title":"Injectable organic-inorganic hybrid hydrogels for bone defect repair","volume":"13","author":"Zhang","year":"","journal-title":"Front. Bioeng. Biotechnol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1186\/s12951-025-03295-0","article-title":"Addressing the challenges of infectious bone defects: a review of recent advances in bifunctional biomaterials","volume":"23","author":"Zhang","year":"","journal-title":"J. Nanobiotechnol"},{"key":"B30","doi-asserted-by":"publisher","first-page":"7383","DOI":"10.1038\/s41598-025-92384-z","article-title":"Therapeutic effect of pH responsive Magainin II modified azithromycin plus curcumin micelles in different depth models of MRSA infection","volume":"15","author":"Zhang","year":"","journal-title":"Sci. Rep."},{"key":"B31","doi-asserted-by":"publisher","first-page":"101647","DOI":"10.1016\/j.mtbio.2025.101647","article-title":"3D-printed intelligent photothermal conversion Nb2C MXene composite scaffolds facilitate the regulation of angiogenesis-osteogenesis coupling for vascularized bone regeneration","volume":"31","author":"Zhang","year":"","journal-title":"Mater. Today Bio"},{"key":"B32","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1039\/D4BM01181C","article-title":"Fast bone regeneration using injectable fully biomimetic organoids with biomineralized and organic microenvironments","volume":"13","author":"Zheng","year":"2025","journal-title":"Biomater. Sci."},{"key":"B33","doi-asserted-by":"publisher","first-page":"139835","DOI":"10.1016\/j.ijbiomac.2025.139835","article-title":"Integrated GelMA and liposome composite hydrogel with effective coupling of angiogenesis and osteogenesis for promoting bone regeneration","volume":"297","author":"Zhu","year":"2025","journal-title":"Int. J. Biol. Macromol."}],"container-title":["Frontiers in Bioengineering and Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2025.1611639\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T05:26:41Z","timestamp":1747805201000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2025.1611639\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,21]]},"references-count":33,"alternative-id":["10.3389\/fbioe.2025.1611639"],"URL":"https:\/\/doi.org\/10.3389\/fbioe.2025.1611639","relation":{},"ISSN":["2296-4185"],"issn-type":[{"value":"2296-4185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,21]]},"article-number":"1611639"}}