{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:58:44Z","timestamp":1788850724438,"version":"build-2803163510"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>To purify increasing amounts of biotherapeutics more efficiently, the use of high flow rates or greater resin bed heights during downstream chromatography steps is a tantalizing option. A limitation of utilizing high flow rates is the differential pressure generated by packed chromatography resin beds. As a resin bed height increases, the resin is susceptible to compression. By increasing the permeability of a packed resin bed through control of the hydraulic radius, column pressure-flow dynamics can be improved. Chromatography column performance using a commercially available Protein A resin was assessed with and without OMEGA, a column insert designed to modulate the hydraulic radius of the column by providing vertical supports through the packed resin bed. OMEGA was shown to reduce the effective hydraulic radius of packed resin beds, increase the permeability of packed columns by 44%\u201373%, and yield a 42%\u201350% decrease in pressure differential across the resin bed at a comparable linear velocity. The structural support provided by OMEGA enables higher operational flow rates and increased resin bed height without impact to either dynamic binding capacity or purified product quality. With the OMEGA column insert, scale-up hurdles are mitigated, and faster downstream processing times are unlocked across column geometries.<\/jats:p>","DOI":"10.3389\/fbioe.2025.1613174","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T09:24:57Z","timestamp":1749115497000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Unlocking efficiency in column chromatography with packed bed supporting inserts"],"prefix":"10.3389","volume":"13","author":[{"given":"Romone M.","family":"Fancy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cole","family":"McMullin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas S.","family":"Brann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel M.","family":"Bailey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David I.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather Bethea","family":"Horne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie S.","family":"Wolfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"key":"B1","unstructured":"Chromatographic bed insert\n          \n          \n            \n              Bailey\n              D. M.\n            \n            \n              Guido\n              S.\n            \n          \n          \n          2024"},{"key":"B2","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1021\/bp950029k","article-title":"A modified version of the volume-averaged continuum theory to predict pressure drop across compressible packed beds of sepharose big-beads SP","volume":"12","author":"Colby","year":"1996","journal-title":"Biotechnol. Prog."},{"key":"B3","article-title":"Les Fontaines Publiques de La Ville de Dijon: Exposition et Application Des Principes \u00e0 Suivre et Des Formules \u00e0 Employer Dans Les Questions de Distribution d\u2019eau","volume":"1","author":"Darcy","year":"1856"},{"key":"B4","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/B978-0-08-100623-8.00026-8","article-title":"Chapter 26 - chromatography columns","volume-title":"Biopharmaceutical processing","author":"Gebauer","year":"2018"},{"key":"B5","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.ces.2015.02.010","article-title":"Evaluation of a structural mechanics model to predict the effect of inserts in the bed support of chromatographic columns","volume":"129","author":"Gerontas","year":"2015","journal-title":"Chem. Eng. Sci."},{"key":"B6","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/S0021-9673(98)01068-1","article-title":"Evidence of a wall friction effect in the consolidation of beds of packing materials in chromatographic columns","volume":"835","author":"Guiochon","year":"1999","journal-title":"J. Chromatogr. A"},{"key":"B7","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/S0021-9673(96)00642-5","article-title":"Consolidation of particle beds and packing of chromatographic columns","volume":"762","author":"Guiochon","year":"1997","journal-title":"J. Chromatogr. A"},{"key":"B8","doi-asserted-by":"publisher","first-page":"2000192","DOI":"10.1002\/biot.202000192","article-title":"Optimization of single-column batch and multicolumn continuous protein A chromatography and performance comparison based on mechanistic model","volume":"15","author":"Guo","year":"2020","journal-title":"Biotechnol. J."},{"key":"B9","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1002\/btpr.2291","article-title":"Continuous bind-and-elute protein A capture chromatography: optimization under process scale column constraints and comparison to batch operation","volume":"32","author":"Kaltenbrunner","year":"2016","journal-title":"Biotechnol. Prog."},{"key":"B10","article-title":"Recovery of biological products","author":"Kanani","year":"2018"},{"key":"B11","article-title":"Bioprocess int","author":"Kanani","year":"2019"},{"key":"B12","article-title":"Predictive algorithm modeling for early assessments in downstream processing: using direct transition and moment analysis to assess","author":"Kim","year":"2023","journal-title":"Chromatogr. Column Integr. A. T. Prod. Scale"},{"key":"B13","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/B978-0-08-100623-8.00016-5","article-title":"Chapter 16 - introduction to preparative protein chromatography","volume-title":"Biopharmaceutical processing","author":"\u0141\u0105cki","year":"2018"},{"key":"B14","doi-asserted-by":"publisher","first-page":"6943","DOI":"10.1016\/j.chroma.2011.08.008","article-title":"Exploration of overloaded cation exchange chromatography for monoclonal antibody purification","volume":"1218","author":"Liu","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"B15","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.jbiotec.2018.07.022","article-title":"Scale-up of continuous multicolumn chromatography for the protein a capture step: from bench to clinical manufacturing","volume":"281","author":"\u00d6tes","year":"2018","journal-title":"J. Biotechnol."},{"key":"B16","first-page":"2","article-title":"Validation of column-based chromatography processes for the purification of proteins. Technical report No. 14","volume":"62","year":"2008","journal-title":"PDA J. Pharm. Sci. Technol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1115\/1.3173119","article-title":"Steady flow in porous, elastically deformable materials","volume":"54","author":"Parker","year":"1987","journal-title":"J. Appl. Mech."},{"key":"B18","doi-asserted-by":"publisher","first-page":"461117","DOI":"10.1016\/j.chroma.2020.461117","article-title":"Pressure-flow experiments, packing, and modeling for scale-up of a mixed mode chromatography column for biopharmaceutical manufacturing","volume":"1625","author":"Prentice","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"B19","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1080\/15422119.2023.2226128","article-title":"The transition from resin chromatography to membrane adsorbers for protein separations at industrial scale","author":"Qu","year":"2023","journal-title":"Sep. and Purif. Rev."},{"key":"B20","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1007\/s10529-018-2552-1","article-title":"Recent developments in chromatographic purification of biopharmaceuticals","volume":"40","author":"Rathore","year":"2018","journal-title":"Biotechnol. Lett."},{"key":"B21","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1007\/s10035-021-01197-0","article-title":"Assessing the wall effects of packed concentric cylinders and angular walls on granular bed porosity","volume":"24","author":"Riess","year":"2022","journal-title":"Granul. Matter"},{"key":"B22","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1007\/s004490050363","article-title":"The effects of processing scale on the pressure drop of compressible gel supports in liquid chromatographic columns","volume":"17","author":"Soriano","year":"1997","journal-title":"Bioprocess Eng."},{"key":"B23","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1021\/bp010060o","article-title":"Pressure-flow relationships for packed beds of compressible chromatography media at laboratory and production scale","volume":"17","author":"Stickel","year":"2001","journal-title":"Biotechnol. Prog."},{"key":"B24","doi-asserted-by":"publisher","first-page":"130134","DOI":"10.1016\/j.jhydrol.2023.130134","article-title":"Revisiting the concept of hydraulic radius","volume":"625","author":"Wei","year":"2023","journal-title":"J. Hydrology"},{"key":"B25","doi-asserted-by":"publisher","first-page":"462451","DOI":"10.1016\/j.chroma.2021.462451","article-title":"Exploration of fiber-based cation exchange adsorbents for the removal of monoclonal antibody aggregates","volume":"1654","author":"Winderl","year":"2021","journal-title":"J. Chromatogr. A"}],"container-title":["Frontiers in Bioengineering and Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2025.1613174\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T09:24:59Z","timestamp":1749115499000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2025.1613174\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,5]]},"references-count":25,"alternative-id":["10.3389\/fbioe.2025.1613174"],"URL":"https:\/\/doi.org\/10.3389\/fbioe.2025.1613174","relation":{},"ISSN":["2296-4185"],"issn-type":[{"value":"2296-4185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,5]]},"article-number":"1613174"}}