{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:12:52Z","timestamp":1785341572992,"version":"3.55.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of <jats:italic>H. pylori<\/jats:italic> is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of <jats:italic>H. pylori<\/jats:italic> in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by <jats:italic>in-situ<\/jats:italic> reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and <jats:italic>H. pylori<\/jats:italic> was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect <jats:italic>H. pylori<\/jats:italic> in a wide concentration range (10<jats:sup>1<\/jats:sup>\u00a0CFU\u00a0mL<jats:sup>\u22121<\/jats:sup>\u201310<jats:sup>8<\/jats:sup>\u00a0CFU\u00a0mL<jats:sup>\u22121<\/jats:sup>) with a low detection limit of 3\u00a0CFU\u00a0mL<jats:sup>\u22121<\/jats:sup>. What\u2019s more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis.<\/jats:p>","DOI":"10.3389\/fbioe.2025.1619336","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T05:47:17Z","timestamp":1752126437000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiaojuan","family":"You","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyi","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huadong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuesheng","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"342491","DOI":"10.1016\/j.aca.2024.342491","article-title":"Sandwich-type electrochemical aptasensor based on hemin-graphite oxide as a signal label and RGO\/mWCNTs\/chitosan\/carbon quantum dot modified electrode for sensitive detection of Acinetobacter baumannii bacteria","volume":"1303","author":"Abedi","year":"2024","journal-title":"Anal. 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