{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:10:23Z","timestamp":1787026223600,"version":"build-2736575974"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Human-derived biomaterials offer several advantages over animal-derived or synthetic alternatives, including improved biocompatibility, ethical acceptability, sustainability, and clinical translatability. Here we present new applications of human placenta-derived materials \u2013 specifically HUMAN PLACENTA substrate, collagen type-I, and Laminin-111 \u2013 as 2D coating materials and 3D matrices for the cultivation of spheroids and adherent cells. Collagen type-I coatings supported colorectal cancer spheroid formation without the need for growth-factor supplementation. Lm-111 significantly enhanced NIH3T3 fibroblast adhesion compared with poly-L-lysine and rat-tail collagen type-I, performing comparably to bovine fibronectin. In a transwell blood\u2013brain barrier model, HUMAN PLACENTA substrate coatings enabled confluent endothelial monolayers with transendothelial electrical resistance values not significantly different from the conventional human collagen type-IV\/bovine fibronectin mixture. Across these\n                    <jats:italic>in vitro<\/jats:italic>\n                    models, placenta-derived materials performed comparably or better than conventional animal-derived and synthetic coatings, supporting robust cell viability, adhesion, and barrier formation. Due to their human origin, these biomaterials exhibit reduced biological complexity while enhancing biocompatibility and translational relevance. Therefore, they provide a sustainable, ethically acceptable alternative for advanced cell culture systems.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2025.1676369","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T06:28:27Z","timestamp":1767335307000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["New applications of sustainable, scalable, standardized, and cost-effective human biomaterials for cell-based assays, tissue engineering, and regenerative medicine"],"prefix":"10.3389","volume":"13","author":[{"given":"Rebekka J. S.","family":"Salzmann","sequence":"first","affiliation":[{"name":"Maternal and Child Health Department Pediatric Hematology, Oncology and Stem Cell Transplant Centre, University of Padua","place":["Padua, Italy"]},{"name":"Istituto di Ricerca Pediatrica \u201cCitt\u00e0 della Speranza\u201d","place":["Padua, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Domazet","sequence":"additional","affiliation":[{"name":"Department of Pathology, Medical University of Vienna","place":["Vienna, Austria"]},{"name":"Ludwig Boltzmann Institute Applied Diagnostics","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Prado-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Institute of Solid-State Electronics, Faculty of Electrical Engineering and Information Technology, TU Wien","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyda","family":"Kigili","sequence":"additional","affiliation":[{"name":"Institute of Solid-State Electronics, Faculty of Electrical Engineering and Information Technology, TU Wien","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winfried","family":"Neuhaus","sequence":"additional","affiliation":[{"name":"Competence Unit Molecular Diagnostics, Centre for Health and Bioresources, AIT - Austrian Institute of Technology GmbH","place":["Vienna, Austria"]},{"name":"Faculty of Medicine and Dentistry, Danube Private University","place":["Krems, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Brachner","sequence":"additional","affiliation":[{"name":"Competence Unit Molecular Diagnostics, Centre for Health and Bioresources, AIT - Austrian Institute of Technology GmbH","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerda","family":"Egger","sequence":"additional","affiliation":[{"name":"Department of Pathology, Medical University of Vienna","place":["Vienna, Austria"]},{"name":"Ludwig Boltzmann Institute Applied Diagnostics","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Moroni","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University","place":["Maastricht, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Hackethal","sequence":"additional","affiliation":[{"name":"THT Biomaterials GmbH","place":["Vienna, Austria"]},{"name":"Austrian Cluster for Tissue Regeneration","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1811","DOI":"10.3390\/ijms25031811","article-title":"Xeno-free 3D bioprinted liver model for hepatotoxicity assessment","volume":"25","author":"Ali","year":"2024","journal-title":"Int. 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