{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:27:17Z","timestamp":1791937637666,"version":"4.3.4"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Potentilla anserina L.<\/jats:italic>\n                      , a traditional Chinese medicinal herb, is valued for its edible, medicinal, and ornamental properties.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, flavonoids were extracted from\n                      <jats:italic>Potentilla anserina L.<\/jats:italic>\n                      using ultrasonic-assisted extraction. The extraction process was optimized through response surface methodology, followed by separation and purification using Sephadex G-100 gel chromatography. Finally, the antioxidant activity of the extracted flavonoids was evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that the optimal extraction conditions were 60% ethanol concentration, an ultrasonic temperature of 50\u00b0C, ultrasonic power of 400 W, and ultrasonic time of 180 min. Under these conditions, the average extraction yield of flavonoids from\n                      <jats:italic>Potentilla anserina L.<\/jats:italic>\n                      was 3.74 \u00b1 0.06 mg\/g. The crude flavonoid extract was purified by Sephadex G-100 gel chromatography, yielding two fractions, LF-1 and LF-2, which accounted for 63.34% and 25.79% of the crude extract, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      The results of in vitro antioxidant activity experiments demonstrated that both fractions (LF-1 and LF-2) exhibited significant antioxidant activity, showing a dose-dependent capacity. These findings provide a theoretical basis for the further development and utilization of flavonoids from\n                      <jats:italic>Potentilla anserina L..<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2025.1691606","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T06:38:15Z","timestamp":1763534295000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Extraction, purification and antioxidant activity of flavonoids from Potentilla anserina L"],"prefix":"10.3389","volume":"13","author":[{"given":"Dandan","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuankang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danqi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenchen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingchen","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1007\/s42452-020-2256-8","article-title":"Hesperidin: synthesis and characterization of bioflavonoid complex","volume":"2","author":"Binkowska","year":"2020","journal-title":"SN Appl. 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