{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:01:00Z","timestamp":1791961260378,"version":"4.3.4"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Vinca alkaloids, a class of naturally derived antimitotic agents isolated from\n                    <jats:italic>Catharanthus roseus<\/jats:italic>\n                    , have long been established as potent chemotherapeutic drugs for the treatment of various malignancies. Despite their clinical efficacy, the therapeutic utility of vinca alkaloids, such as vincristine, vinblastine, and vinorelbine, is frequently constrained by systemic toxicity, poor bioavailability, and the emergence of multidrug resistance. In this study, we developed a universal strategy to construct cell membrane-derived vesicles for the encapsulation of vinca alkaloids, thereby enhancing their antitumor efficacy. These artificial cell vesicles were fabricated through the extraction and reconstitution of membranes from K562 cells. Following optimization of drug-loading efficiency, the resulting therapeutic vesicles, designated membrane-encapsulated vinca alkaloids (M@VAs), were thoroughly characterized to evaluate their drug delivery performance. An optimally formulated vincristine-loaded vesicle (M@vincristine) was subsequently used to assess its antitumor efficacy both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . M@vincristine induced a dose-dependent reduction in cell viability and demonstrated significantly greater tumor suppression than free vincristine. At the administered dose, M@vincristine not only promoted enhanced apoptosis but also modulated the expression of key apoptotic factors and effectively induced cell cycle arrest in the M phase. Following intravenous administration, M@vincristine demonstrated efficient tumor accumulation and superior anti-tumor efficacy compared to free vincristine. This artificial therapeutic platform not only addresses the major limitations associated with the clinical use of vinca alkaloids but also paves the way for the development of novel therapeutic agents with broad clinical applicability.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2025.1703637","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T05:42:01Z","timestamp":1760506921000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Artificial cell vesicle-mediated delivery of Catharanthus roseus (L.) G. Don-derived vinca alkaloids for enhanced antitumor efficacy"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiaodong","family":"Zhuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangjiu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyue","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"36","DOI":"10.22034\/nmbj.2023.389869.1017","article-title":"Recent progresses and challenges in formulations of vincristine and its derivatives for hindering cancer cells","volume":"2","author":"Ahmadi","year":"2023","journal-title":"Nano Micro Biosyst."},{"key":"B2","article-title":"A Comprehensive Review on Catharanthus roseus L. (G.) Don: Clinical Pharmacology","author":"Chaturvedi","year":"2022"},{"key":"B3","doi-asserted-by":"publisher","first-page":"9582","DOI":"10.1039\/d4cs00411f","article-title":"Proteolysis-targeting drug delivery system (ProDDS): integrating targeted protein degradation concepts into formulation design","volume":"53","author":"Chen","year":"2024","journal-title":"Chem. Soc. Rev."},{"key":"B4","doi-asserted-by":"publisher","first-page":"11","DOI":"10.3389\/fbioe.2023.1177151","article-title":"Advances in nanomaterial-based targeted","author":"Cheng","year":"2023","journal-title":"drug Deliv. Syst."},{"key":"B5","doi-asserted-by":"publisher","first-page":"4476","DOI":"10.1039\/c3nr33891f","article-title":"Unprecedented inhibition of tubulin polymerization directed by gold nanoparticles inducing cell cycle arrest and apoptosis","volume":"5","author":"Choudhury","year":"2013","journal-title":"Nanoscale."},{"key":"B6","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.actbio.2025.05.041","article-title":"Vincristine\/Volasertib polymersome injection enables high-efficiency synergistic treatment of acute lymphoblastic leukemia","volume":"200","author":"Deng","year":"2025","journal-title":"Acta Biomater."},{"key":"B7","first-page":"11","volume-title":"Dual inhibition of \u03b3-tubulin and Plk1 induces mitotic cell death","author":"Ebisu","year":"2021"},{"key":"B8","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.ijbiomac.2019.12.158","article-title":"A tropomyosin-like Meretrix meretrix Linnaeus polypeptide inhibits the proliferation and metastasis of glioma cells via microtubule polymerization and FAK\/Akt\/MMPs signaling","volume":"145","author":"Fan","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1021\/nn900002m","article-title":"Impact of Nanotechnology on drug delivery","volume":"3","author":"Farokhzad","year":"2009","journal-title":"ACS Nano"},{"key":"B10","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1016\/j.jconrel.2020.10.005","article-title":"A6 peptide-tagged, ultra-small and reduction-sensitive polymersomal vincristine sulfate as a smart and specific treatment for CD44+ acute myeloid leukemia","volume":"329","author":"Gu","year":"2021","journal-title":"J. Control. Release"},{"key":"B11","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1038\/s41590-017-0013-y","article-title":"Oxeiptosis, a ROS-induced caspase-independent apoptosis-like cell-death pathway","volume":"19","author":"Holze","year":"2018","journal-title":"Nat. Immunol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1946","DOI":"10.1038\/cdd.2015.59","article-title":"Identification of synthetic lethality of PLK1 inhibition and microtubule-destabilizing drugs","volume":"22","author":"Hugle","year":"2015","journal-title":"Cell Death and Differ."},{"key":"B13","doi-asserted-by":"publisher","first-page":"114647","DOI":"10.1016\/j.jep.2021.114647","article-title":"Catharanthus roseus (L.) G. Don: a review of its ethnobotany, phytochemistry, ethnopharmacology and toxicities","volume":"284","author":"Kumar","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"2096","DOI":"10.1016\/j.apsb.2021.01.020","article-title":"Cell membrane-derived vesicles for delivery of therapeutic agents","volume":"11","author":"Le","year":"2021","journal-title":"Acta Pharm. Sin. B"},{"key":"B15","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1038\/s41578-020-00269-6","article-title":"Targeted drug delivery strategies for precision medicines","volume":"6","author":"Manzari","year":"2021","journal-title":"Nat. Rev. Mater."},{"key":"B16","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.jconrel.2020.08.012","article-title":"Controlled anti-cancer drug release through advanced nano-drug delivery systems: Static and dynamic targeting strategies","volume":"327","author":"Moradi Kashkooli","year":"2020","journal-title":"J. Control. Release"},{"key":"B17","first-page":"1231","article-title":"Vinca alkaloids","volume":"4","author":"Moudi","year":"2013","journal-title":"Int. J. Prev. Med."},{"key":"B18","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1007\/s40820-020-00482-6","article-title":"A review on nano-based drug delivery system for cancer Chemoimmunotherapy","volume":"12","author":"Mu","year":"2020","journal-title":"Nano-Micro Lett."},{"key":"B19","doi-asserted-by":"publisher","first-page":"5187","DOI":"10.1021\/acsnano.2c11965","article-title":"Advances in drug delivery systems based on red blood cells and their membrane-derived nanoparticles","volume":"17","author":"Nguyen","year":"2023","journal-title":"ACS Nano"},{"key":"B20","doi-asserted-by":"publisher","first-page":"11562","DOI":"10.1039\/d3tb02118a","article-title":"Improving nanochemoimmunotherapy efficacy by boosting \u201ceat-me\u201d signaling and downregulating \u201cdon't-eat-me\u201d signaling with Ganoderma lucidum polysaccharide-based drug delivery","volume":"11","author":"Pang","year":"2023","journal-title":"J. Mater. Chem. B"},{"key":"B21","doi-asserted-by":"publisher","first-page":"131","DOI":"10.22271\/plants.2024.v12.i3b.1675","article-title":"A review on phytochemical constituents and pharmacological properties of Catharanthus roseus (L.) G. Don","volume":"12","author":"Sharma","year":"2024","journal-title":"J. Med. Plants Stud."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s13346-023-01389-6","article-title":"Vincristine-based nanoformulations: a preclinical and clinical studies overview","volume":"14","author":"Shukla","year":"2024","journal-title":"Drug Deliv. Transl. Res."},{"key":"B23","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1023\/a:1009616228304","article-title":"Role of reactive oxygen species (ROS) in apoptosis induction","volume":"5","author":"Simon","year":"2000","journal-title":"Apoptosis"},{"key":"B24","doi-asserted-by":"publisher","first-page":"863","DOI":"10.7150\/thno.11852","article-title":"Cell or cell membrane-based drug delivery systems","volume":"5","author":"Tan","year":"2015","journal-title":"Theranostics"},{"key":"B25","doi-asserted-by":"publisher","first-page":"101144","DOI":"10.1016\/j.mtbio.2024.101144","article-title":"An intratumor bacteria-targeted DNA nanocarrier for multifaceted tumor microenvironment intervention","volume":"27","author":"Wang","year":"2024","journal-title":"Mater. Today Bio"},{"key":"B26","doi-asserted-by":"publisher","first-page":"2304824","DOI":"10.1002\/smll.202304824","article-title":"Nanoghosts: Harnessing Mesenchymal stem cell membrane for construction of drug delivery platforms via optimized biomimetics","volume":"20","author":"Xiao","year":"2024","journal-title":"Small"},{"key":"B27","doi-asserted-by":"publisher","first-page":"11301","DOI":"10.1073\/pnas.1107723108","article-title":"The function of mammalian Polo-like kinase 1 in microtubule nucleation","volume":"108","author":"Xu","year":"2011","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"B28","doi-asserted-by":"publisher","first-page":"2102330","DOI":"10.1002\/advs.202102330","article-title":"Engineered cell membrane-derived nanoparticles in immune modulation","volume":"8","author":"Yang","year":"2021","journal-title":"Adv. Sci."},{"key":"B29","doi-asserted-by":"publisher","first-page":"6059","DOI":"10.2147\/ijn.s502144","article-title":"Advancements in cell membrane-derived biomimetic Nanotherapeutics for breast cancer","volume":"20","author":"Zeng","year":"2025","journal-title":"Int. J. Nanomedicine"},{"key":"B30","doi-asserted-by":"publisher","first-page":"220","DOI":"10.7150\/thno.87425","article-title":"Red blood cells in biology and translational medicine: natural vehicle inspires new biomedical applications","volume":"14","author":"Zhang","year":"2024","journal-title":"Theranostics"},{"key":"B31","doi-asserted-by":"publisher","first-page":"164657","DOI":"10.1016\/j.cej.2025.164657","article-title":"Polymersomal vincristine-PLK1 inhibitor boosts immunogenic cell death and antitumor immunity","volume":"518","author":"Zhao","year":"2025","journal-title":"Chem. Eng. J."}],"container-title":["Frontiers in Bioengineering and Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2025.1703637\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T05:42:03Z","timestamp":1760506923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2025.1703637\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,15]]},"references-count":31,"alternative-id":["10.3389\/fbioe.2025.1703637"],"URL":"https:\/\/doi.org\/10.3389\/fbioe.2025.1703637","relation":{},"ISSN":["2296-4185"],"issn-type":[{"value":"2296-4185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,15]]},"article-number":"1703637"}}