{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T07:43:28Z","timestamp":1784447008764,"version":"3.55.0"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Knowledge about extremophile organisms and their survival strategies could be of great value for various industrial applications including biological plastics recycling. The black fungus\n                    <jats:italic>Knufia chersonesos<\/jats:italic>\n                    inhabits extreme environments such as rocks and therefore produces specific enzymes that function under harsh conditions. A cutinase (Kc_Cut) and a lipase (Kc_Lip) identified\n                    <jats:italic>via<\/jats:italic>\n                    proteomics-based screening in the secretome of\n                    <jats:italic>K. chersonesos<\/jats:italic>\n                    grown on poly (butylene-adipate-\n                    <jats:italic>co<\/jats:italic>\n                    -terephthalate) (PBAT) as carbon sources were recombinantly expressed in\n                    <jats:italic>Komagataella phaffii<\/jats:italic>\n                    and\n                    <jats:italic>Trichoderma reesei<\/jats:italic>\n                    , respectively, for further characterization. The purified enzymes showed a specific activity of 83 \u00b1 1 and 0.23 \u00b1 0.02 U\u00a0mg\n                    <jats:sup>-1<\/jats:sup>\n                    on\n                    <jats:italic>para<\/jats:italic>\n                    -nitrophenylbutyrate (\n                    <jats:italic>p<\/jats:italic>\n                    -NPB) as substrate, respectively. Optimum conditions of Kc_Cut were evaluated through activity measurements on\n                    <jats:italic>p<\/jats:italic>\n                    -NPB and resulted in 50\u00a0\u00b0C, pH 8 and 100\u00a0mM potassium phosphate buffer. Incubation with PBAT powder for 72\u00a0h resulted in the release of 17 \u00b1 1\u00a0\u00b5M of terephthalic acid (Ta) by Kc_Cut while the Kc_Lip liberated the trimer BTaB. This indicated a cooperative action of the two enzymes which was confirmed by hydrolysis of BTaB by Kc_Cut and provides valuable insight into the metabolic potential and adaptability of\n                    <jats:italic>K. chersonesos<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2025.1720118","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T08:10:35Z","timestamp":1768205435000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Life on the rocks: unexpected enzyme activity of the extremophilic black fungus Knufia chersonesos"],"prefix":"10.3389","volume":"13","author":[{"given":"Sophia","family":"Mihalyi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Zimmermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felice","family":"Quartinello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Tesei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid R.","family":"Mach-Aigner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Guebitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Ribitsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"E4350","DOI":"10.1073\/pnas.1718804115","article-title":"Characterization and engineering of a plastic-degrading aromatic polyesterase","volume":"115","author":"Austin","year":"2018","journal-title":"Proc. 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