{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T08:36:58Z","timestamp":1775032618277,"version":"3.50.1"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Resin-based sealants (RBSs) are widely used to prevent pit and fissure caries in clinical dentistry. However, their high density of C=C double bonds leads to volumetric shrinkage during photo-polymerization, which may compromise enamel bonding. Additionally, the biosafety of commonly used monomers such as bisphenol A glycerolate dimethacrylate (bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) remains a concern due to potential health risks in adolescent patients. This study aimed to develop a bis-GMA- and TEGDMA-free pit and fissure sealant to address these limitations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A novel family of isosorbide-derived dental sealants (ISDSs) was developed using a synthesized isosorbide-based matrix, bis(2-(methacryloyloxy)ethyl) disuccinate isosorbide (IBMEDS), and a diluent, bis(2-methylacrylate) isosorbide (IBM). The performance of ISDS was evaluated against the commercial Clinpro\u2122 sealant in terms of polymerization shrinkage, cytotoxicity, physicochemical properties, microleakage resistance, and stability of the sealed area under artificial aging conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with Clinpro, ISDS exhibited a 34% reduction in polymerization shrinkage and a more than sevenfold increase in L929 cell viability, attributed to its bio-based structure and low double-bond density. Moreover, ISDS demonstrated superior physicochemical properties, including higher shear bond strength, more effective marginal adaptation to enamel, lower water absorption and solubility, and more sustained and stable long-term fluoride release, while maintaining comparable microleakage resistance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>A fully bio-based, fluoride-containing isosorbide-derived dental sealant (ISDS) was developed to overcome the polymerization shrinkage and biocompatibility limitations of conventional resin-based sealants. This novel sealant offers enhanced long-term stability, excellent biocompatibility, improved marginal adaptation, and sustained fluoride release, representing a safer and more effective alternative for caries prevention in children and adolescents.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2026.1730749","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T05:43:17Z","timestamp":1775022197000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and pre-clinical evaluation of an isosorbide-based pit and fissure sealant achieving low polymerization shrinkage and high biocompatibility"],"prefix":"10.3389","volume":"14","author":[{"given":"Ming","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinan","family":"Cui","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baiyan","family":"Sui","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Sun","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"CD001830","DOI":"10.1002\/14651858.CD001830.pub4","article-title":"Sealants for preventing dental decay in the permanent teeth","volume":"3","author":"Ahovuo-Saloranta","year":"2013","journal-title":"Cochrane Database Syst. 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