{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T06:14:25Z","timestamp":1776147265189,"version":"3.50.1"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001738","name":"Ministry of Innovation, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Decellularized extracellular matrix (dECM) materials are widely reported to present tissue-specific biochemical cues that influence cell behavior; here, we use porcine uterine dECM as a representative soft-tissue model to operationalize a practical digital light printing workflow. Digital light processing (DLP) offers high-fidelity, photopolymer-based fabrication that avoids shear stresses associated with extrusion and enables precise layer definition, yet its application to soft-tissue dECM remains limited. We produce and evaluate a standalone methacrylated dECM (dECM-MA) formulation and a stepwise, reproducible recipe using lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and tartrazine. Histological and biochemical analyses confirm successful decellularization, substantial collagen retention, partial sGAG retention, and controlled methacrylation of accessible primary amine groups. The formulation prints via DLP to yield reproducibly defined acellular constructs at a 50-\u00b5m layer height and millimeter-scale geometries, demonstrating high dimensional fidelity and controlled swelling behavior. High resolution scanning electron microscopy (HR-SEM) imaging of printed ECM-MA and solid decellularized uterine tissue demonstrated no significant differences in porosity, pore size distribution profiles, and connectivity, as quantified by image analysis, suggesting similar capacity to support diffusion and cell penetration.\n                    <jats:italic>In vitro<\/jats:italic>\n                    studies with human uterine stromal fibroblasts\u2014parenchymal cells of the endometrium\u2014show surface attachment and early cell\u2013matrix interaction on printed constructs. Together, these results establish a practical 405-nm digital light printing workflow for standalone methacrylated dECM, exemplified using uterine ECM, enabling acellular construct stereolithographic fabrication with preserved ECM features and compatibility with early cell attachment and histological processing.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1774476","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T05:39:56Z","timestamp":1776145196000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Standalone methacrylated extracellular matrix for digital light processing bioprinting: a practical workflow"],"prefix":"10.3389","volume":"14","author":[{"given":"Hod","family":"Bruck","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering, Ariel University","place":["Ariel, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shachar","family":"Sofer","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering, Ariel University","place":["Ariel, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aharon","family":"Lion","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering, Ariel University","place":["Ariel, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asher","family":"Ornoy","sequence":"additional","affiliation":[{"name":"The Department of Morphological Sciences and Teratology, The Dr. Miriam and Sheldon Adelson School of Medicine, Ariel University","place":["Ariel, Israel"]},{"name":"Department of Medical Neurobiology, Faculty of Medicine, The Hebrew University of Jerusalem","place":["Jerusalem, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udi","family":"Sarig","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering, Ariel University","place":["Ariel, Israel"]},{"name":"The Department of Morphological Sciences and Teratology, The Dr. Miriam and Sheldon Adelson School of Medicine, Ariel University","place":["Ariel, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1038\/s41563-025-02329-0","article-title":"Local photocrosslinking of native tissue matrix regulates lung epithelial cell mechanosensing and function","volume":"24","author":"Ahmed","year":"2025","journal-title":"Nat. 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