{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:32:02Z","timestamp":1787725922245,"version":"build-2784847793"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Critically low blood donation rates and recurrent shortages in the blood supply have become pressing challenges in transfusion medicine. Xenotransfusion using porcine red blood cells (pRBCs) represents a promising alternative; however, risks of immune rejection and infection necessitate the development of genetically engineered porcine models \u2013 a process that is time-consuming, costly, and technically complex. In this study, we established an immortalized porcine erythroid progenitor cell line as a platform for genetic manipulation and the evaluation of xenotransfusion potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Porcine hematopoietic progenitor cells (CD117\n                      <jats:sup>+<\/jats:sup>\n                      ) were isolated from bone marrow mononuclear cells and immortalized via lentiviral transduction with E6\/E7 oncogenes from Human papillomavirus type 16 (HPV16) and Sus scrofa papillomavirus (SPV2).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The resulting cells were optimized for long-term culture and exhibited stable proliferation. The immortalized porcine erythroid progenitor cells retained the capacity to differentiate into red blood cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Our findings demonstrate that E6\/E7-mediated immortalization facilitates the generation of porcine erythroid progenitor cells capable of differentiating into red blood cells, offering a valuable tool for genetic engineering and a promising avenue toward safe, sustainable heterologous blood transfusion strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2026.1807068","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:04:22Z","timestamp":1787724262000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of an immortalized porcine erythroid progenitor cell line using species-adapted papillomavirus oncogenes"],"prefix":"10.3389","volume":"14","author":[{"given":"Tra My","family":"Tran","sequence":"first","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeon Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Hyeon","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Hyuk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhat Minh","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Guk","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joohyun","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Transgenic Animal Research, Optipharm, Inc.","place":["Cheongju-si, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Ho","family":"Hwang","sequence":"additional","affiliation":[{"name":"Division of Advanced Predictive Research, Center for Bio-Signal Research, Korea Institute of Toxicology (KIT)","place":["Daejeon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University","place":["Seongnam, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/CELLS10030523","article-title":"Direct generation of immortalized erythroid progenitor cell lines from peripheral blood mononuclear cells","volume":"10","author":"Bagchi","year":"2021","journal-title":"Cells"},{"key":"B2","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.ijsu.2015.07.684","article-title":"Recent advances in genome editing and creation of genetically modified pigs","volume":"23","author":"Butler","year":"2015","journal-title":"Int. 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