{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:00:05Z","timestamp":1790348405280,"version":"4.1.0"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Bacterial pigments have broad industrial applications and are relatively easy to scale up. However, Arctic microbial sources remain largely unexplored. In this study, we isolated a yellow-pigmented bacterium, PS104, from the Arctic Ny-\u00c5lesund fjord in Kongsfjorden, Svalbard, Norway. Biochemical assays and 16S rRNA gene sequencing revealed that PS104 is closely related to the genus\n                    <jats:italic>Citricoccus<\/jats:italic>\n                    . Pigment characterization using UV\u2013visible spectroscopy showed a carotenoid-like profile with a three-shoulder absorption peak, while Fourier transform infrared (FT-IR) spectroscopy and high-resolution liquid chromatography mass spectrophotometry (HR-LCMS) analysis confirmed the presence of yellow carotenoid pigment consistent with zeaxanthin (C\n                    <jats:sub>40<\/jats:sub>\n                    H\n                    <jats:sub>56<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ), along with additional unidentified peaks. To enhance the pigment yield, we employed a multi-stage optimization strategy beginning with one-factor-at-a-time, followed by response surface methodology (RSM), which resulted in \u223c200% increases in pigment dry weight from 26\u00a0mg\/100\u00a0mL to 78\u00a0mg\/100\u00a0mL. Further supplementation of RSM media with TiO\n                    <jats:sub>2<\/jats:sub>\n                    nanomaterials provided an additional \u223c30% increase, achieving 102\u00a0mg\/100\u00a0mL. Overall, the optimized conditions resulted in a \u223c3.9-fold improvement compared to basal media. Early findings demonstrate that MDCK cells exhibit no negative response to the pigment, highlighting its promise for textile dyeing applications. This research demonstrates the successful isolation of an Arctic\n                    <jats:italic>Citricoccus<\/jats:italic>\n                    -like strain and systematic optimization of its carotenoid pigment yield, providing preliminary evidence of its biotechnological potential, particularly for textile coloration.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1824301","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:16:13Z","timestamp":1790345773000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Physico-chemical factors and nanomaterial-mediated optimization of pigment yield from a psychrotrophic marine Citricoccus-like isolate of an Arctic fjord"],"prefix":"10.3389","volume":"14","author":[{"given":"Rakshita","family":"Kaskar","sequence":"first","affiliation":[{"name":"School of Biological Sciences and Biotechnology, Goa University","place":["Taleigao, Goa, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Tiwari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Biotechnology, Goa University","place":["Taleigao, Goa, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghanath","family":"Prabhu","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Biotechnology, Goa University","place":["Taleigao, Goa, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savita","family":"Kerkar","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Biotechnology, Goa University","place":["Taleigao, Goa, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhermendra K.","family":"Tiwari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Biotechnology, Goa University","place":["Taleigao, Goa, India"]},{"name":"University Grant Commission (UGC)","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"128","DOI":"10.3390\/md12010128","article-title":"Astaxanthin: sources, extraction, stability, biological activities and its commercial applications\u2014A review","volume":"12","author":"Ambati","year":"2014","journal-title":"Mar. 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