{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:35:05Z","timestamp":1790807705435,"version":"4.1.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010882","name":"Tianjin Municipal Education Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010882","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012580","name":"Natural Science Foundation of Tianjin-Science and Technology Correspondent Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012580","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Hydrogels are widely used as wound dressings owing to their biocompatibility, high water content, and ability to mimic extracellular matrix functions. Alginate (ALG) is a natural polysaccharide that forms ionically crosslinked hydrogels in the presence of calcium, where Ca\n                    <jats:sup>2+<\/jats:sup>\n                    not only stabilizes the crosslinked network but also regulates cell behavior, which is essential for wound repair. In this study, ALG hydrogels were prepared with CaCl\n                    <jats:sub>2<\/jats:sub>\n                    at 20, 50, 100, and 200\u00a0mM, and their gelation, rheological properties, calcium ion release, and cellular responses were systematically evaluated. The 100\u00a0mM CaCl\n                    <jats:sub>2<\/jats:sub>\n                    formulation exhibited the optimal mechanical stability and bioactivity, markedly promoting fibroblast proliferation, migration, and extracellular matrix organization\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Application of this hydrogel to a rat model with full-thickness skin defects significantly accelerated wound closure and tissue regeneration. Transcriptomic analysis further confirmed activation of the calcium signaling pathway in fibroblasts. These findings highlight the pivotal role of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in orchestrating fibroblast activity, providing an optimized alginate-based dressing for effective wound healing.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1828848","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:29:34Z","timestamp":1781843374000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Calcium ions released from alginate hydrogel promote wound healing by enhancing fibroblast activity"],"prefix":"10.3389","volume":"14","author":[{"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Medicine, Nankai University","place":["Tianjin, China"]},{"name":"Department of Clinical Laboratory, Gansu Provincial Hospital","place":["Lanzhou, China"]},{"name":"Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Nankai University Affiliated Third Center Hospital","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Luo","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Central Hospital, Tianjin University\/Tianjin Third Central Hospital","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubo","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Nankai University Affiliated Third Center Hospital","place":["Tianjin, China"]},{"name":"The Third Central Clinical College of Tianjin Medical University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiting","family":"Deng","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Central Hospital, Tianjin University\/Tianjin Third Central Hospital","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingtang","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Nankai University Affiliated Third Center Hospital","place":["Tianjin, China"]},{"name":"Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Central Hospital, Tianjin University\/Tianjin Third Central Hospital","place":["Tianjin, China"]},{"name":"Artificial Cell Engineering Technology Research Center","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"4163","DOI":"10.3390\/polym15204163","article-title":"Influence of alginate properties and calcium chloride concentration on alginate bead reticulation and size: a phenomenological approach","volume":"15","author":"Bennacef","year":"2023","journal-title":"Polym. 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