{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T06:05:43Z","timestamp":1784009143141,"version":"3.55.0"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Lignin recalcitrance, together with its complex interactions with cellulose and hemicelluloses within the plant cell wall, remains a major barrier to efficient lignocellulosic biomass valorization. Mild alkaline pretreatment partially addresses this challenge by solubilizing lignin and acetate into a lignin-rich alkaline liquor (AL). However, the recovered lignin is largely oligomeric, limiting its direct microbial conversion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To evaluate strategies for improving AL bioconversion, alkaline liquor was subjected to thermochemical depolymerization under different severities, generating liquors with compositions ranging from oligomer-rich to monomer-rich profiles. The resulting streams were evaluated through two bioconversion routes: aerobic metabolism by\n                      <jats:italic>Pseudomonas putida<\/jats:italic>\n                      KT2440 and anaerobic digestion for biomethane production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mild to moderate depolymerization conditions (\u2264240 \u00b0C) improved the growth of\n                      <jats:italic>P. putida<\/jats:italic>\n                      , whereas the highest depolymerization severity strongly inhibited bacterial growth. This inhibition was associated with increased concentrations of aromatic monomers that are poorly metabolized by\n                      <jats:italic>P. putida<\/jats:italic>\n                      , including phenol and alkyl-substituted phenols. In anaerobic digestion assays, mild to moderate depolymerization yielded the highest specific methane productions, corresponding to a 10%\u201320% increase relative to non-depolymerized AL, while severe depolymerization conditions negatively affected methane production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings demonstrate that controlled lignin depolymerization can enhance both aerobic and anaerobic bioconversion by balancing chemical accessibility with biological compatibility. This approach provides a promising strategy for improving lignin utilization within integrated biorefinery concepts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2026.1837262","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T05:39:11Z","timestamp":1784007551000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of lignin depolymerization on aerobic and anaerobic bioconversion of alkaline liquor from sugarcane bagasse"],"prefix":"10.3389","volume":"14","author":[{"given":"Fabr\u00edcia","family":"Farias de Menezes","sequence":"first","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Miyuki","family":"Kashiwagi","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Jacinta","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology (ICT), Federal University of Alfenas (UNIFAL)","place":["Po\u00e7os de Caldas, Minas Gerais, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Rodrigues","family":"Gomes","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela","family":"Prata","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Rosa de Moraes","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano Freitas","family":"Lima","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Garcia da Silva","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Piacentini","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology (ICT), Federal University of Alfenas (UNIFAL)","place":["Po\u00e7os de Caldas, Minas Gerais, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo","family":"Driemeier","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Jackson de Moraes Rocha","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila Oliveira","family":"de Giuseppe","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)","place":["Campinas, S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"127479","DOI":"10.1016\/j.biortech.2022.127479","article-title":"Pseudomonas sp. 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