{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:01:15Z","timestamp":1785477675110,"version":"3.56.0"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Myocardial infarction is one of the most significant causes of death worldwide and the limited regenerative capacity of the myocardium often results in poor recovery after injury. Stem cell therapy has some promise for myocardial tissue repair, but clinical translation is hindered by poor cell survival and safety concerns, prompting the investigations of cell-free strategies such as stem cell-derived secretome or lysate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, a quaternized chitosan\/tannic acid hydrogel was fabricated and characterized for its crosslinking stability, injectability and degradation kinetics. In addition, mouse adipose-derived mesenchymal stem cells (mADSCs) were isolated, characterized, and processed into cell-free lysate. The mADSCs lysate was then analyzed by TEM, NTA, Western blot and enzyme-linked immunosorbent assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>It was found that the mADSCs lysate contained abundant vesicles and a broad panel of cytokines\/growth factors with anti-inflammatory, pro-angiogenic and antioxidant properties. Encapsulating the lysate within the hydrogel showed sustained release. The hydrogel-based delivery of mADSCs lysate could promote high metabolic activity and good cell number increases of H9c2-cardiomyocytes, L929-fibroblasts and human umbilical vein endothelial cells, demonstrating great potential for myocardial tissue repair applications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      By integrating the regenerative potential of ADSCs lysate with this biocompatible, injectable hydrogel platform, this combined strategy circumvents the potential safety issues associated with live cell transplantation and offers a scalable, potential cell-free delivery strategy for myocardial repair-related applications, laying a foundation for future\n                      <jats:italic>in vivo<\/jats:italic>\n                      studies and further translational evaluation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2026.1855161","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:37:28Z","timestamp":1785476248000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A hydrogel platform delivering mesenchymal stem cell lysate for potential myocardial tissue repair"],"prefix":"10.3389","volume":"14","author":[{"given":"Ziwei","family":"Du","sequence":"first","affiliation":[{"name":"International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuhua","family":"Wang","sequence":"additional","affiliation":[{"name":"International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Santerre","sequence":"additional","affiliation":[{"name":"International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University","place":["Yantai, Shandong, China"]},{"name":"Institute of Biomedical Engineering, University of Toronto","place":["Toronto, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Ma","sequence":"additional","affiliation":[{"name":"Li Ka Shing Faculty of Medicine, The University of Hong Kong","place":["Pokfulam, Hong Kong SAR, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Ma","sequence":"additional","affiliation":[{"name":"International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University","place":["Yantai, Shandong, China"]},{"name":"Institute of Biomedical Engineering, University of Toronto","place":["Toronto, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.3390\/pharmaceutics13101686","article-title":"Chitosan nanoparticles at the biological interface: implications for drug delivery","volume":"13","author":"Aibani","year":"2021","journal-title":"Pharmaceutics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.matbio.2023.06.007","article-title":"TSG6 hyaluronan matrix remodeling dampens the inflammatory response during colitis","volume":"121","author":"Albtoush","year":"2023","journal-title":"Matrix Biol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1551791","DOI":"10.3389\/fbioe.2025.1551791","article-title":"Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs","author":"An","year":"2025","journal-title":"Front. 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