{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:11:07Z","timestamp":1784182267690,"version":"3.55.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Reliable and efficient isolation of tumor-associated cells from peripheral blood remains a significant challenge due to their low abundance and heterogeneity. Here, we present a physics-enabled and fully-automated approach based on microsieve technology for rapid separation and capture of these cells from whole blood. The approach integrates periodic backflush to dynamically disrupt pore blockage and an anti-fouling surface coating to suppress cell adhesion, thereby establishing a self-regulating filtration mechanism. Implemented in a fully automated microsieve system, this mechanism enables processing of 3 mL of whole blood within 25\u00a0min, with &amp;gt;90% recovery of viable cancer cells and &amp;gt;99.99% depletion of white blood cells. Compared with other membrane-based filtration methods, the proposed approach ensures more efficient and consistent isolation of cancer cells, demonstrating feasibility for future clinical translation.<\/jats:p>","DOI":"10.3389\/fbioe.2026.1867527","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T05:40:17Z","timestamp":1784180417000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["High-throughput cancer cell isolation via dynamic anti-clogging microsieve filtration"],"prefix":"10.3389","volume":"14","author":[{"given":"Min","family":"Hu","sequence":"first","affiliation":[{"name":"Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laiyi","family":"Lin","sequence":"additional","affiliation":[{"name":"Grabtaxi Holdings Pte. 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