{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T06:18:26Z","timestamp":1787811506234,"version":"build-2784847793"},"reference-count":23,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Dengue virus (DENV) infection poses a serious threat to global public health. Rapid and sensitive point-of-care diagnostic methods are urgently needed for early detection and epidemic control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The dengue virus monoclonal antibody NS1-McAb1# was labeled as the labeled antibody and sprayed onto the binding pad using ruthenium tripyridine as the signal probe, and the dengue virus monoclonal antibody DN-REAB-G2-006 was fixed on the detection pad as the capture antibody. To mitigate potential interference from other substances in the sample, the sample pad and conjugate pad was pretreated with a solution containing heterophilic blocking reagent, and Elecsys buffer was selected as the optimal co-reactant. After dropping the sample onto the sample pad, chromatography and immunoreaction were performed sequentially. Then, cyclic voltammetry scanning was carried out in the test zone at a rate of 0.1\u00a0V\/s for electrochemiluminescence (ECL) detection. A novel detection method was established by optimizing various parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      The ruthenium-labeled antibody was prepared by reacting Ru-NHS ester with NS1-McAb1# at a molar ratio of 50:1 for 10\u00a0h. Elecsys Buffer was selected as the co-reactant, with a sample-to-buffer volume ratio of 2:1. Whatman AE 100 membrane was used as the detection pad with a length set to 15\u00a0mm. The concentration ratio of labeled antibody to capture antibody was 1:2. The chromatographic time after sample loading was 10\u00a0min. Under these optimized conditions, the detection method achieved a minimum detection limit of 20.76\u00a0pg\/mL, exhibited good specificity, with an intra-assay repeatability coefficient of variation of 4%, a storage stability coefficient of variation of 0.83%, and an inter-assay stability coefficient of variation of 0.81%. Clinical sample validation showed a\n                      <jats:italic>Kappa<\/jats:italic>\n                      value of 0.625 compared with nucleic acid testing results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The developed ECLIA test strip provides a portable, rapid, and sensitive detection method for DENV, with potential applications in on-site testing across multiple scenarios. Although preliminary clinical evaluation showed acceptable concordance with nucleic acid testing, further large-scale validation using diverse clinical specimens is necessary to fully establish its diagnostic utility in real-world settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2026.1881809","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:35:08Z","timestamp":1787808908000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of electrochemiluminescence immunochromatographic test strips for dengue virus"],"prefix":"10.3389","volume":"14","author":[{"given":"Qinyi","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Public Health, Guangdong Medical University","place":["Zhanjiang, China"]},{"name":"Department of Basic Medical Research, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Qin","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Medical University","place":["Zhanjiang, China"]},{"name":"Department of Basic Medical Research, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xihan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Research, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liting","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Medical University","place":["Zhanjiang, China"]},{"name":"Department of Basic Medical Research, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanshan","family":"Mai","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Research, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wu","sequence":"additional","affiliation":[{"name":"Zhuhai Center for Disease Control and Prevention","place":["Zhuhai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Research, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Medical University","place":["Zhanjiang, China"]},{"name":"Guangdong Food and Drug Vocational College","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"117373","DOI":"10.1016\/j.bios.2025.117373","article-title":"Transformative biomedical devices to overcome biomatrix effects","volume":"279","author":"Adil","year":"2025","journal-title":"Biosens. 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