{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T07:00:47Z","timestamp":1785308447088,"version":"3.55.0"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Skin wound healing remains a major clinical challenge due to impaired regeneration and incomplete restoration of dermal architecture, creating the need for biomaterials that reconstruct the human extracellular matrix (ECM) microenvironment, especially the collagen-rich structural network. Current strategies to obtain human-relevant collagen primarily include genetically engineered recombinant collagen and ECM directly produced by human cells. Although recombinant collagen has been extensively developed, it represents a simplified surrogate of native human collagen, and the biosynthesis of fully mature human collagen remains technically challenging. Therefore, we developed an engineered and scalable strategy to fabricate human cell-derived engineered ECM (HE-ECM) from human fibroblasts and systematically compared it with commercial recombinant collagen. Physicochemical analyses demonstrated that HE-ECM preserved native-like porous matrix architecture, collagen triple-helical integrity, enhanced thermal stability, and a more complex protein composition. HE-ECM significantly enhanced fibroblast viability, proliferation, and migration\n                    <jats:italic>in vitro<\/jats:italic>\n                    and accelerated wound closure with improved tissue organization in a rat full-thickness skin defect model. Overall, this controllable and donor-independent fabrication strategy enables scalable production of human collagen-rich HE-ECM that more faithfully recapitulates the native skin microenvironment than recombinant collagen, thereby suggesting its potential as a biomimetic platform for tissue regeneration.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1892416","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:03:42Z","timestamp":1785305022000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["An engineered and scalable human cell-derived extracellular matrix demonstrates advantages over recombinant collagen in biomimicry and wound healing"],"prefix":"10.3389","volume":"14","author":[{"given":"Xiaolei","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tribology, Tsinghua University","place":["Beijing, China"]},{"name":"Center for Medical Device Evaluation, National Medical Products Administration","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoye","family":"Ran","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Huaqing Zhimei (Shenzhen) Biotechnology Co., Ltd.","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boxun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Huaqing Zhimei (Shenzhen) Biotechnology Co., Ltd.","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengbo","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Huaqing Zhimei (Shenzhen) Biotechnology Co., Ltd.","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqing","family":"Zang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiadao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tribology, Tsinghua University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Huaqing Zhimei (Shenzhen) Biotechnology Co., Ltd.","place":["Shenzhen, China"]},{"name":"Bio-Intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua University in Shenzhen, Tsinghua University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.biomaterials.2016.02.003","article-title":"Extracellular matrix-based biomaterial scaffolds and the host response","volume":"86","author":"Aamodt","year":"2016","journal-title":"Biomaterials"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.1016\/j.biomaterials.2006.12.030","article-title":"Biomechanical and biochemical characteristics of a human fibroblast-produced and remodeled matrix","volume":"28","author":"Ahlfors","year":"2007","journal-title":"Biomaterials"},{"key":"B3","doi-asserted-by":"publisher","first-page":"602009","DOI":"10.3389\/fbioe.2020.602009","article-title":"Cell-derived extracellular matrix for tissue engineering and regenerative medicine","volume":"8","author":"Assun\u00e7\u00e3o","year":"2020","journal-title":"Front. 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