{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:18:46Z","timestamp":1787912326167,"version":"build-2784847793"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Mitochondria provide a unique enriched acetyl coenzyme A reservoir and an ideal redox environment, offering an efficient supply for the biosynthesis of terpene precursors and terpene products. This comprehensive review focuses on the engineering of mitochondrial compartmentalization in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    to enhance terpene production. We systematically outline the applications from hemiterpenes to tetraterpenes and elaborate on how advanced strategies, such as key enzyme mitochondrial targeting strategy, whole-pathway mitochondrial targeting strategy, dual cytoplasmic-mitochondrial compartmentalization strategy, and multi-organelle synergistic compartmentalization strategy, can effectively improve precursor availability while mitigating metabolic interference and cytotoxic effects. In addition, we critically examine the discussions and long-term challenges associated with these methods. Finally, we highlight the transformative potential of mitochondrial compartmentalization to pioneer future innovations in complex terpene synthesis engineering.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1910253","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:09:17Z","timestamp":1787911757000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Advances in mitochondrial compartmentalization for terpenoid synthesis in Saccharomyces cerevisiae"],"prefix":"10.3389","volume":"14","author":[{"given":"Chuyi","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Southern University of Science and Technology","place":["Shenzhen, Guangdong, China"]},{"name":"Shenzhen University of Advanced Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Ma","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3387","DOI":"10.1093\/bioinformatics\/btx431","article-title":"DeepLoc: prediction of protein subcellular localization using deep learningHancock J","volume":"33","author":"Almagro Armenteros","year":"2017","journal-title":"Bioinformatics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1038\/s41589-024-01759-2","article-title":"ML-enhanced peroxisome capacity enables compartmentalization of multienzyme pathway","volume":"21","author":"Baker","year":"2025","journal-title":"Nat. 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