{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T05:05:50Z","timestamp":1789967150227,"version":"4.0.1"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Global poultry egg production reached 100 million tonnes in 2024, and eggshell membranes account for approximately 10% of total egg mass. Mass egg consumption produces enormous amounts of waste eggshell. Eggshell is a natural calcium source, while eggshell membrane contains abundant proteins; both can be further processed into high value-added products. Current eggshell-membrane separation technologies commonly suffer from high energy input, chemical pollution or low separation efficiency, restricting large-scale industrial application. To address this bottleneck, this work adopts a combined shearing-grinding crushing strategy, which makes full use of the brittle nature of eggshell and high toughness of eggshell membrane. Key components of a custom shearing-grinding separation device were innovatively designed for this process. A four-factor three-level Box-Behnken response surface methodology (RSM) was implemented, with eggshell membrane separation rate selected as the response indicator for parameter optimization. The RSM-BBD regression model exhibited acceptable fitting performance (adjusted R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.8637, predicted R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.6983, Adequate Precision = 12.729). Under optimized operating conditions (15% moisture content, motor speed of 1,000 r\/min, feed mass of 51\u00a0g, rotor-stator gap of 1\u00a0mm), a maximum separation rate of 87.28% was achieved. The proposed shearing-grinding device features simple structure, low energy consumption and environmental compatibility. This study offers a feasible technical route for the efficient industrial separation of waste eggshell and eggshell membrane, and supports the high-value valorization of poultry biomass waste within a circular economy framework.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1928807","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:29:24Z","timestamp":1789964964000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Design and optimization of a shearing grinding device for efficient separation of eggshell and eggshell membrane"],"prefix":"10.3389","volume":"14","author":[{"given":"Yuan","family":"Chi","sequence":"first","affiliation":[{"name":"College of Engineering, Northeast Agricultural University","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabao","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Engineering, Northeast Agricultural University","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Engineering, Northeast Agricultural University","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Engineering, Northeast Agricultural University","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Chi","sequence":"additional","affiliation":[{"name":"College of Food Science, Northeast Agricultural University","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1007\/s11033-024-09394-9","article-title":"Harnessing potential of Avian eggshell membrane derived collagen hydrolysate for bone tissue regeneration","volume":"51","author":"Aggarwal","year":"2024","journal-title":"Mol. 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