{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T06:05:00Z","timestamp":1789452300166,"version":"build-2803163510"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Activated platelet\u2010rich plasma (aPRP) reduces inflammation and promotes regeneration in osteoarthritic knee joints. It may exert therapeutic effects on osteoarthritic chondrocytes (OACs) compared with healthy chondrocytes (HCs) by promoting cell proliferation, reducing autophagy\u2010related vesicles and inhibiting apoptosis through modulation of intracellular signaling pathways. The aim of this study was therefore to evaluate cell viability, apoptotic fate, extracellular matrix protein expression (collagen type II (COL2A1) and aggrecan (ACAN)), autophagy\u2010related vesicle production, and growth factor profiles, including TGF\u2010\u03b2 and PDGF-BB, in HCs and OACs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mechanically aPRP\u2010S and PRP\u2010G using mini-beads, leukocyte-rich L\u2010PRP, leukocyte-poor P\u2010PRP and platelet-rich in growth factors (PRGF) were co-cultured with HCs and OACs for real-time proliferation analysis, apoptosis by flow cytometry, autophagy and related intracellular markers through immunofluorescent labeling, and the secretome profile by ELISA, aligned with their effective dose (ED50) and optimal ED50 time point.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>aPRPs at 2.7% in 2 h and PRGF at 1.8% in 3 h induced proliferation of OACs. PRGF reduced the apoptotic cell ratios of OACs. aPRPs and PRGF promoted COL2A1 and ACAN protein synthesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The induction of proliferation of the OACs is a promising result that can be translated to clinical applications. aPRPs may have a stimulatory role in OACs. This role can be dose and preparation protocol dependent. aPRPs and PRGF can be preferred to stimulate COL2A1 synthesis and reduce apoptotic cell ratios in cultured OACs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fbioe.2026.1940844","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:36:15Z","timestamp":1789450575000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Activated platelet rich plasma modulates the proliferation, apoptosis and matrix synthesis driven by growth factors in osteoarthritic chondrocytes: an in vitro study"],"prefix":"10.3389","volume":"14","author":[{"given":"\u00d6zge","family":"Boyac\u0131o\u011flu","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, At\u0131l\u0131m University","place":["Ankara, T\u00fcrkiye"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilge Ba\u015fak","family":"Fidan","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Graduate School of Science and Engineering, Hacettepe University","place":["Ankara, T\u00fcrkiye"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"\u00c7elebier","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University","place":["Ankara, T\u00fcrkiye"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petek","family":"Korkusuz","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Hacettepe University","place":["Ankara, T\u00fcrkiye"]},{"name":"Metu Mems Center","place":["Ankara, T\u00fcrkiye"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feza","family":"Korkusuz","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Faculty of Medicine, Hacettepe University","place":["Ankara, T\u00fcrkiye"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"139","DOI":"10.2478\/s11535-013-0255-0","article-title":"Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro","volume":"9","author":"Amrichov\u00e1","year":"2014","journal-title":"Central Eur. 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