{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:05:01Z","timestamp":1789617901224,"version":"build-2803163510"},"reference-count":159,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Effective management of drug-resistant tuberculosis requires molecular assays that not only detect\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    but also identify resistance-associated variants, support drug-level interpretation, and indicate when broader confirmatory testing is needed. This narrative review applies a mutation-to-drug-to-decision framework to distinguish assay validity, exact-variant detection, drug-level inference, and decision impact. We synthesize evidence on target accessibility, minority-allele preservation, multiplexing, direct-specimen testing, and sequencing enrichment, emphasizing how these factors shape clinical interpretability. Current evidence remains dominated by analytical studies, isolate-based evaluations, and small direct-specimen investigations, with prospective multicenter treatment-impact validation still lacking. Population-level benchmarks indicate incomplete biological coverage for compact target regions, reaching approximately 92.6% for\n                    <jats:italic>rpoB<\/jats:italic>\n                    RRDR-based rifampicin detection, 85.4% for\n                    <jats:italic>katG<\/jats:italic>\n                    codon 315 plus the\n                    <jats:italic>inhA<\/jats:italic>\n                    promoter for isoniazid, and 84.7%\u201388.2% for\n                    <jats:italic>gyrA\/gyrB<\/jats:italic>\n                    -based fluoroquinolone detection. Allele-specific CRISPR is therefore best suited to rapid rule-in of selected resistance-associated variants, while dispersed or heterogeneous mechanisms require broader molecular or phenotypic assessment. CRISPR should complement WHO-recommended rapid molecular testing and targeted next-generation sequencing rather than replace established molecular or phenotypic methods.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1947704","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:49:11Z","timestamp":1789616951000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["From mutation detection to treatment decisions: CRISPR-enabled diagnostics for drug-resistant tuberculosis"],"prefix":"10.3389","volume":"14","author":[{"given":"Weijia","family":"Yi","sequence":"first","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fukang","family":"Luo","sequence":"additional","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianping","family":"Yi","sequence":"additional","affiliation":[{"name":"The Fourth People\u2019s Hospital of Jintang (Jintang County Mental Health Center)","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"Genetic Diseases Key Laboratory of Sichuan Province, Department of Medical Genetics, Sichuan Academy of Medical Sciences and Sichuan Provincial People\u2019s Hospital, University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibing","family":"Yan","sequence":"additional","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhi","family":"Luo","sequence":"additional","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1007\/s40291-024-00741-x","article-title":"Mycobacterium tuberculosis detection using CRISPR technology: an updated systematic review and meta-analysis","volume":"28","author":"Abavisani","year":"2024","journal-title":"Mol. 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