{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:23:46Z","timestamp":1789118626280,"version":"build-2803163510"},"reference-count":128,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (Kp) is a major cause of healthcare-associated and community-onset invasive infections. The emergence of carbapenem-resistant Kp (CRKP), hypervirulent Kp (hvKp), and resistance\u2013hypervirulence convergence has increased the need for rapid tests that provide organism context and clinically interpretable risk information. CRISPR\/Cas diagnostics combine programmable sequence recognition with flexible signal generation and can be integrated with isothermal amplification, portable readouts, and multiplex workflows. Available studies support the analytical feasibility of detecting Kp- or\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    species complex (KpSC)-associated organism-context targets, major resistance genes, and hypervirulence-associated markers, but clinical and workflow validation remains limited because many studies use small cohorts, cultured isolates, or spiked matrices. Here, clinically interpretable risk reporting means translating validated molecular findings into bounded categories\u2014organism context, gene-associated resistance risk, hypervirulence-associated risk, and suspected resistance\u2013hypervirulence convergence risk\u2014while stating interpretive limits and appropriate confirmatory actions. We critically evaluate CRISPR\/Cas engineering, target selection, multiplexing, specimen-specific workflows, and validation requirements, and propose a conceptual four-layer target-to-report framework for prospective evaluation. CRISPR\/Cas-based Kp testing should complement, rather than replace, culture, phenotypic antimicrobial susceptibility testing, virulence assessment, and genomic confirmation.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1949504","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:58:17Z","timestamp":1789117097000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["CRISPR\/Cas diagnostics for Klebsiella pneumoniae: from resistance and hypervirulence markers to clinically interpretable risk reporting"],"prefix":"10.3389","volume":"14","author":[{"given":"Weijia","family":"Yi","sequence":"first","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhi","family":"Luo","sequence":"additional","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fukang","family":"Luo","sequence":"additional","affiliation":[{"name":"West China Hospital Sichuan University Jintang Hospital, Jintang First People\u2019s Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianyi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Public Health Clinical Center of Chengdu","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Third Affiliated Hospital of Zunyi Medical University (The First People\u2019s Hospital of Zunyi)","place":["Zunyi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiujie","family":"Yu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guozhen","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Neurology, Sichuan Provincial People\u2019s Hospital, University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"Genetic Diseases Key Laboratory of Sichuan Province, Department of Medical Genetics, Sichuan Academy of Medical Sciences and Sichuan Provincial People\u2019s Hospital, University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1021\/acssynbio.9b00507","article-title":"Nucleic acid detection using CRISPR\/Cas biosensing technologies","volume":"9","author":"Aman","year":"2020","journal-title":"ACS Synth. 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