{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:49:53Z","timestamp":1791355793790,"version":"4.3.1"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>\n                    Recombinant protein production can be a challenging process. To help users troubleshoot and optimize experiments in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    we have compiled and characterized a toolbox of biosensor plasmids. It contains a biosensor that monitors the translational efficiency of the recombinant protein and quality control biosensors that detect unwanted events, such as protein misfolding in the cytoplasm, inefficient translocation through the Sec translocon, or protein misfolding in the periplasm. The biosensors were combined with different fluorescent proteins so that multiple events could be measured in parallel, in the same cell. The toolbox is a resource for diagnosing protein production problems and evaluating solutions that mitigate these problems.\n                  <\/jats:p>","DOI":"10.3389\/fbioe.2026.1956994","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:53:29Z","timestamp":1791352409000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A biosensor toolbox for monitoring recombinant protein production in Escherichia coli"],"prefix":"10.3389","volume":"14","author":[{"given":"Maurice","family":"M\u00fcrk\u00f6ster","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateusz","family":"Balka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina J.","family":"Kamp","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"K\u00e4llstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linus","family":"Karlsson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia A.","family":"Rodriguez Aparicio","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Tun\u00e4ng","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna-Louise","family":"\u00d6gren","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Theopold","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alister J.","family":"Cumming","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Toftgaard Nielsen","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Biotechnology Research Institute for the Green Transition (BRIGHT), Technical University of Denmark","place":["Lyngby, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel O.","family":"Daley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Stockholm University","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1128\/AEM.64.6.2240-2246.1998","article-title":"New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria","volume":"64","author":"Andersen","year":"1998","journal-title":"Appl. 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