{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T19:43:51Z","timestamp":1780083831109,"version":"3.54.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T00:00:00Z","timestamp":1606262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872322"],"award-info":[{"award-number":["31872322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31601859"],"award-info":[{"award-number":["31601859"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2662017PY030"],"award-info":[{"award-number":["2662017PY030"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>The<jats:italic>H19<\/jats:italic>gene promotes skeletal muscle differentiation in mice, but the regulatory models and mechanisms of myogenesis regulated by<jats:italic>H19<\/jats:italic>are largely unknown in pigs. Therefore, the regulatory modes of<jats:italic>H19<\/jats:italic>in the differentiation of porcine skeletal muscle satellite cells (PSCs) need to be determined. We observed that<jats:italic>H19<\/jats:italic>gene silencing could decrease the expressions of the myogenin (<jats:italic>MYOG<\/jats:italic>) gene, myogenic differentiation (<jats:italic>MYOD<\/jats:italic>), and myosin heavy chain (<jats:italic>MYHC<\/jats:italic>) in PSCs. Therefore, we constructed and sequenced 12 cDNA libraries of PSCs after knockdown of<jats:italic>H19<\/jats:italic>at two differentiation time points to analyze the transcriptome differences. A total of 11,419 differentially expressed genes (DEGs) were identified. Among these DEGs, we found through bioinformatics analysis and protein interaction experiment that SRY-box transcription factor 4 (<jats:italic>SOX4<\/jats:italic>) and Drebrin 1 (<jats:italic>DBN1<\/jats:italic>) were the key genes in<jats:italic>H19<\/jats:italic>-regulated PSC differentiation. Functional analysis shows that<jats:italic>SOX4<\/jats:italic>and<jats:italic>DBN1<\/jats:italic>promote PSC differentiation. Mechanistically,<jats:italic>H19<\/jats:italic>regulates PSC differentiation through two different pathways. On the one hand,<jats:italic>H19<\/jats:italic>functions as a molecular sponge of<jats:italic>miR-140-5p<\/jats:italic>, which inhibits the differentiation of PSCs, thereby modulating the derepression of<jats:italic>SOX4<\/jats:italic>. On the other hand,<jats:italic>H19<\/jats:italic>regulates PSC differentiation through directly binding with DBN1. Furthermore,<jats:italic>MYOD<\/jats:italic>binds to the promoters of<jats:italic>H19<\/jats:italic>and<jats:italic>DBN1<\/jats:italic>. The knockdown of<jats:italic>MYOD<\/jats:italic>inhibits the expression of<jats:italic>H19<\/jats:italic>and<jats:italic>DBN1<\/jats:italic>. We determined the function of<jats:italic>H19<\/jats:italic>and provided a molecular model to elucidate<jats:italic>H19<\/jats:italic>\u2019s role in regulating PSC differentiation.<\/jats:p>","DOI":"10.3389\/fcell.2020.518724","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T07:17:51Z","timestamp":1606288671000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Long Non-coding RNA H19 Regulates Porcine Satellite Cell Differentiation Through miR-140-5p\/SOX4 and DBN1"],"prefix":"10.3389","volume":"8","author":[{"given":"Jingxuan","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhe","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoli","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengchi","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1242\/dev.068353","article-title":"Myoblast fusion: lessons from flies and mice.","volume":"139","author":"Abmayr","year":"2012","journal-title":"Development"},{"key":"B2","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2013a Python framework to work with high-throughput sequencing data.","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1242\/dev.084665","article-title":"Myod and H19-Igf2 locus interactions are required for diaphragm formation in the mouse.","volume":"140","author":"Borensztein","year":"2013","journal-title":"Development"},{"key":"B4","doi-asserted-by":"publisher","first-page":"3997","DOI":"10.1128\/MCB.25.10.3997-4009.2005","article-title":"MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex.","volume":"25","author":"de la Serna","year":"2005","journal-title":"Mol. 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