{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:40:44Z","timestamp":1790440844496,"version":"4.1.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 31872331"],"award-info":[{"award-number":["No. 31872331"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"DOI":"10.3389\/fcell.2020.541260","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T08:52:21Z","timestamp":1599814341000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["C2C12 Mouse Myoblasts Damage Induced by Oxidative Stress Is Alleviated by the Antioxidant Capacity of the Active Substance Phloretin"],"prefix":"10.3389","volume":"8","author":[{"given":"Jie","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanying","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuzhao","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianyong","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,9,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1007\/s12192-018-0888-zwi","article-title":"Antioxidant effect of myricitrin on hyperglycemia-induced oxidative stress in C2C12 cell.","volume":"23","author":"Ahangarpour","year":"2018","journal-title":"Cell Stress Chaperones."},{"key":"B2","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1208\/s12248-017-0053-0","article-title":"Phloretin prevents high-fat diet-induced obesity and improves metabolic homeostasis.","volume":"19","author":"Alsanea","year":"2017","journal-title":"AAPS J."},{"key":"B3","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1080\/03610730701525352","article-title":"Effects of apple consumption on plasma and erythrocyte antioxidant parameters in elderly subjects.","volume":"33","author":"Avci","year":"2007","journal-title":"Exp. Aging. Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/j.bbamcr.2018.02.010","article-title":"Nrf2-Keap1 signaling in oxidative and reductive stress.","volume":"1865","author":"Bellezza","year":"2018","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.freeradbiomed.2016.04.001","article-title":"Mitochondrial generation of superoxide and hydrogen peroxide as the source of mitochondrial redox signaling.","volume":"100","author":"Brand","year":"2016","journal-title":"Free Radic. Biol. Med."},{"key":"B6","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1007\/978-981-13-1435-3_29","article-title":"Muscle atrophy: present and future.","volume":"1088","author":"Cao","year":"2018","journal-title":"Adv. Exp. Med. Biol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.ceb.2017.01.005","article-title":"AMPK signalling in health and disease.","volume":"45","author":"Carling","year":"2017","journal-title":"Curr. Opin. Cell. Biol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1152\/physiolgenomics.00041.2017","article-title":"Nrf2 at the heart of oxidative stress and cardiac protection.","volume":"50","author":"Chen","year":"2018","journal-title":"Physiol. Genomics"},{"key":"B9","doi-asserted-by":"publisher","first-page":"12455","DOI":"10.1021\/acs.jafc.8b03407","article-title":"Chlorpyrifos induction of testicular-cell apoptosis through generation of reactive oxygen species and phosphorylation of AMPK.","volume":"66","author":"Chen","year":"2018","journal-title":"J. Agr. Food. Chem."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24020278","article-title":"Biochemical basis of anti-cancer-effects of phloretin-A natural dihydrochalcone.","volume":"24","author":"Choi","year":"2019","journal-title":"Molecules"},{"key":"B11","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1016\/j.biopha.2018.07.035","article-title":"Schisandrin A prevents oxidative stress-induced DNA damage and apoptosis by attenuating ROS generation in C2C12 cells.","volume":"106","author":"Choi","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"B12","doi-asserted-by":"publisher","first-page":"89","DOI":"10.22203\/ecm.v030a07","article-title":"Oxidative stress inhibits the proliferation, induces premature senescence and promotes a catabolic phenotype in human nucleus pulposus intervertebral disc cells.","volume":"30","author":"Dimozi","year":"2015","journal-title":"Eur. Cells Mater."},{"key":"B13","doi-asserted-by":"publisher","first-page":"3073","DOI":"10.1039\/c9fo02334h","article-title":"Activation of AMPK\/Sirt3 pathway by phloretin reduces mitochondrial ROS in vascular endothelium by increasing the activity of MnSOD via deacetylation.","volume":"11","author":"Han","year":"2020","journal-title":"Food Funct."},{"key":"B14","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.jnutbio.2017.08.008","article-title":"Dietary resveratrol confers apoptotic resistance to oxidative stress in myoblasts.","volume":"50","author":"Haramizu","year":"2017","journal-title":"J. Nutr. Biochem."},{"key":"B15","doi-asserted-by":"publisher","first-page":"2677","DOI":"10.1021\/acs.jafc.6b05582","article-title":"Phenolic elderberry extracts, anthocyanins, procyanidins, and metabolites influence glucose and fatty acid uptake in human skeletal muscle cells.","volume":"65","author":"Ho","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"B16","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.intimp.2015.04.024","article-title":"Phloretin ameliorates chemokines and ICAM-1 expression via blocking of the NF-\u03baB pathway in the TNF-\u03b1-induced HaCaT human keratinocytes.","volume":"27","author":"Huang","year":"2015","journal-title":"Int. Immunopharmacol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"408","DOI":"10.3945\/an.111.000513","article-title":"A comprehensive review of apples and apple components and their relationship to human health.","volume":"2","author":"Hyson","year":"2011","journal-title":"Adv. Nutr."},{"key":"B18","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/s00210-017-1449-1","article-title":"Schisandrin C enhances mitochondrial biogenesis and autophagy in C2C12 skeletal muscle cells: potential involvement of anti-oxidative mechanisms.","volume":"391","author":"Kim","year":"2018","journal-title":"N. S. Arch. Pharmacol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1089\/ars.2016.6942","article-title":"Reactive oxygen species\/nitric oxide mediated inter-organ communication in skeletal muscle wasting diseases.","volume":"26","author":"Leitner","year":"2017","journal-title":"Antioxid. Redox. Sign."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23051162","article-title":"Antioxidant structure-activity relationship analysis of five dihydrochalcones.","volume":"23","author":"Li","year":"2018","journal-title":"Molecules"},{"key":"B21","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.fct.2018.03.034","article-title":"Grape seed procyanidin extract protects against Pb-induced lung toxicity by activating the AMPK\/Nrf2\/p62 signaling axis.","volume":"116","author":"Lu","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.semcdb.2016.08.003","article-title":"Muscle redox disturbances and oxidative stress as pathomechanisms and therapeutic targets in early-onset myopathies.","volume":"64","author":"Moulin","year":"2017","journal-title":"Semin. Cell. Dev. Biol."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134420","article-title":"The 5\u2019-AMP-Activated protein kinase (AMPK) is involved in the augmentation of antioxidant defenses in cryopreserved chicken sperm.","volume":"10","author":"Nguyen","year":"2015","journal-title":"PLoS One"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2016.41","article-title":"Flavonoids: an overview.","volume":"5","author":"Panche","year":"2016","journal-title":"J. Nutr. Sci."},{"key":"B25","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.fct.2016.06.004","article-title":"Hepatotoxicity and endothelial dysfunction induced by high choline diet and the protective effects of phloretin in mice.","volume":"94","author":"Ren","year":"2016","journal-title":"Food Chem. Toxicol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.bbrc.2011.04.122","article-title":"Angiotensin II induced catabolic effect and muscle atrophy are redox dependent.","volume":"409","author":"Semprun-Prieto","year":"2011","journal-title":"Biochem. Bioph. Res."},{"key":"B27","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1002\/jcp.24512","article-title":"Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts.","volume":"229","author":"Sondag","year":"2014","journal-title":"J. Cell. Physiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.intimp.2018.08.004","article-title":"Activation of Nrf2 signaling by salvianolic acid C attenuates NF-\u03baB mediated inflammatory response both in vivo and in vitro.","volume":"63","author":"Song","year":"2018","journal-title":"Int. Immunopharmacol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1097\/MAJ.0b013e318222e620","article-title":"Angiotensin II, oxidative stress and skeletal muscle wasting.","volume":"342","author":"Sukhanov","year":"2011","journal-title":"Am. J. Med. Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1829","DOI":"10.1212\/CON.0000000000000403","article-title":"Metabolic myopathies.","volume":"22","author":"Tarnopolsky","year":"2016","journal-title":"Continuum"},{"key":"B31","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.jfda.2016.10.016","article-title":"An apple a day to prevent cancer formation: reducing cancer risk with flavonoids.","volume":"25","author":"Tu","year":"2017","journal-title":"J. Food Drug Anal."},{"key":"B32","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.ejphar.2015.02.036","article-title":"Phloretin ameliorates arsenic trioxide induced mitochondrial dysfunction in H9c2 cardiomyoblasts mediated via alterations in membrane permeability and ETC complexes.","volume":"754","author":"Vineetha","year":"2015","journal-title":"Eur. J. Pharmacol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1097\/FJC.0000000000000213","article-title":"Phloretin inhibits platelet-derived growth factor-BB-induced rat aortic smooth muscle cell proliferation, migration, and neointimal formation after carotid injury.","volume":"65","author":"Wang","year":"2015","journal-title":"J. Cardiovasc. Pharm."},{"key":"B34","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1080\/1028415X.2016.1231438","article-title":"Quercetin attenuates domoic acid-induced cognitive deficits in mice.","volume":"21","author":"Wang","year":"2018","journal-title":"Nutr. Neurosci."},{"key":"B35","doi-asserted-by":"publisher","first-page":"10665","DOI":"10.1007\/s00253-018-9376-8","article-title":"Phloretin reduces cell injury and inflammation mediated by staphylococcus aureus via targeting sortase B and the molecular mechanism.","volume":"102","author":"Wang","year":"2018","journal-title":"Appl. Microbiol. Biot."},{"key":"B36","doi-asserted-by":"publisher","first-page":"16447","DOI":"10.3390\/molecules191016447","article-title":"Synthesis, crystal structure, and biological evaluation of a series of phloretin derivatives.","volume":"19","author":"Wang","year":"2014","journal-title":"Molecules"},{"key":"B37","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.jfda.2017.03.009","article-title":"The apple polyphenol phloretin inhibits breast cancer cell migration and proliferation via inhibition of signals by type 2 glucose transporter.","volume":"26","author":"Wu","year":"2018","journal-title":"J. Food Drug. Anal."},{"key":"B38","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1021\/acs.jafc.8b05025","article-title":"Activation of Nrf2 by phloretin attenuates palmitic acid-induced endothelial cell oxidative stress via AMPK-dependent signaling.","volume":"67","author":"Yang","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00774","article-title":"Phloretin prevents diabetic cardiomyopathy by dissociating Keap1\/Nrf2 complex and inhibiting oxidative stress.","volume":"9","author":"Ying","year":"2018","journal-title":"Front. Endocrinol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1039\/c8fo01699b","article-title":"The protective role of phloretin against dextran sulfate sodium-induced ulcerative colitis in mice.","volume":"10","author":"Zhang","year":"2019","journal-title":"Food Funct."},{"key":"B41","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1002\/jcsm.12281","article-title":"A newly identified lncRNA MAR1 acts as a miR-487b sponge to promote skeletal muscle differentiation and regeneration.","volume":"9","author":"Zhang","year":"2018","journal-title":"J. Cachexia Sarcopeni"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/srep20300","article-title":"Icaritin requires phosphatidylinositol 3 kinase (PI3K)\/Akt signaling to counteract skeletal muscle atrophy following mechanical unloading.","volume":"6","author":"Zhang","year":"2016","journal-title":"Sci. Rep."},{"key":"B43","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1016\/j.bbrc.2017.06.076","article-title":"Fortunellin protects against high fructose-induced diabetic heart injury in mice by suppressing inflammation and oxidative stress via AMPK\/Nrf-2 pathway regulation.","volume":"490","author":"Zhao","year":"2017","journal-title":"Biochem. Biophys. Res."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcell.2020.541260\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T08:52:24Z","timestamp":1599814344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcell.2020.541260\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,11]]},"references-count":43,"alternative-id":["10.3389\/fcell.2020.541260"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2020.541260","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,11]]},"article-number":"541260"}}