{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:34:55Z","timestamp":1791290095797,"version":"4.3.0"},"reference-count":11,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>In this study, we analyzed neural stem cells transfected with the HRE-VEGF gene in groups experiencing different periods of hypoxia. The results of RT-PCR showed that the expression of vascular endothelial growth factor (VEGF) mRNA gradually increased with the prolonged period of hypoxia (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). The results from the western-blot test showed that expression of the VEGF protein increased with as the period of hypoxia increased (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). The results of MTT combined with Elisa reagent showed that with the prolonged period of hypoxia, the secretion of VEGF protein increased, and that the proliferation of target cells and neural stem cells was better promoted (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). These results imply that HRE can safely and effectively regulate VEGF expression. By controlling the period of hypoxia, we can increase the expression level, and limit it in more safe values to avoid the possibility of cancer caused by the over-enhancement of proliferation of target cells due to the overexpression of the VEGF protein.<\/jats:p>","DOI":"10.3389\/fcell.2020.580824","type":"journal-article","created":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T18:38:47Z","timestamp":1610044727000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["The Effect of HRE-Regulated VEGF Expression and Transfection on Neural Stem Cells in Rats"],"prefix":"10.3389","volume":"8","author":[{"given":"Bo","family":"Dou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangrong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danfeng","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xixi","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8100428","article-title":"Differences in tolerance to hypoxia: physiological, biochemical, and molecular-biological characteristics.","volume":"8","author":"Dzhalilova","year":"2020","journal-title":"Biomedicines"},{"key":"B2","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1084\/jem.20180752","article-title":"VEGF expands erythropoiesis via hypoxia-independent induction of erythropoietin in noncanonical perivascular stromal cells.","volume":"216","author":"Greenwald","year":"2019","journal-title":"J. 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