{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:42:05Z","timestamp":1790779325245,"version":"4.1.0"},"reference-count":78,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T00:00:00Z","timestamp":1621468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS072212"],"award-info":[{"award-number":["NS072212"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Lysolecithin is commonly used to induce demyelinating lesions in the spinal cord and corpus callosum of mammalian models. Although these models and clinical patient samples are used to study neurodegenerative diseases, such as multiple sclerosis (MS), they do not allow for direct visualization of disease-related damage <jats:italic>in vivo<\/jats:italic>. To overcome this limitation, we created and characterized a focal lysolecithin injection model in zebrafish that allows us to investigate the temporal dynamics underlying lysolecithin-induced damage <jats:italic>in vivo<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> We injected lysolecithin into 4\u20136 days post-fertilization (dpf) zebrafish larval spinal cords and, coupled with <jats:italic>in vivo<\/jats:italic>, time-lapse imaging, observed hallmarks consistent with mammalian models of lysolecithin-induced demyelination, including myelinating glial cell loss, myelin perturbations, axonal sparing, and debris clearance.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> We have developed and characterized a lysolecithin injection model in zebrafish that allows us to investigate myelin damage in a living, vertebrate organism. This model may be a useful pre-clinical screening tool for investigating the safety and efficacy of novel therapeutic compounds that reduce damage and\/or promote repair in neurodegenerative disorders, such as MS.<\/jats:p>","DOI":"10.3389\/fcell.2021.654583","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T06:52:50Z","timestamp":1621493570000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["A Novel Lysolecithin Model for Visualizing Damage in vivo in the Larval Zebrafish Spinal Cord"],"prefix":"10.3389","volume":"9","author":[{"given":"Angela D.","family":"Morris","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Kucenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,5,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/j.acthis.2004.08.004","article-title":"Ultrastructural and immunohistochemical similarities of two distinct entities; multiple sclerosis and hereditary motor sensory neuropathy.","volume":"106","author":"Acar","year":"2004","journal-title":"Acta Histochem."},{"key":"B2","doi-asserted-by":"publisher","first-page":"4443","DOI":"10.1242\/dev.071001","article-title":"Individual axons regulate the myelinating potential of single oligodendrocytes in vivo.","volume":"138","author":"Almeida","year":"2011","journal-title":"Development"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.1038\/nn738","article-title":"TNF alpha promotes proliferation of oligodendrocyte progenitors and remyelination.","volume":"4","author":"Arnett","year":"2001","journal-title":"Nat. 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