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Cell Dev. Biol."],"abstract":"<jats:p><jats:bold>Purpose:<\/jats:bold> To systematically analyze the overall m<jats:sup>6<\/jats:sup>A modification pattern in hyperplastic scars (HS).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The m<jats:sup>6<\/jats:sup>A modification patterns in HS and normal skin (NS) tissues were described by m<jats:sup>6<\/jats:sup>A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m<jats:sup>6<\/jats:sup>A-related RNA was immunoprecipitated and verified by real-time quantitative PCR.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The appearance of 14,791 new m<jats:sup>6<\/jats:sup>A peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique m<jats:sup>6<\/jats:sup>A-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated m<jats:sup>6<\/jats:sup>A peaks.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> This study is the first to map the m<jats:sup>6<\/jats:sup>A transcriptome of human HS, which may help clarify the possible mechanism of m<jats:sup>6<\/jats:sup>A-mediated gene expression regulation.<\/jats:p>","DOI":"10.3389\/fcell.2021.748703","type":"journal-article","created":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T06:57:59Z","timestamp":1636959479000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar"],"prefix":"10.3389","volume":"9","author":[{"given":"Si-Yu","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Jie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-hua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Li","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-ying","family":"Teng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai-Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Yue","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dang-yang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-Lai","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,11,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"225","DOI":"10.2340\/00015555-2498","article-title":"Burden of keloid disease: a cross-sectional health-related quality of life assessment.","volume":"97","author":"Bijlard","year":"2017","journal-title":"Acta Derm. 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