{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:09:37Z","timestamp":1785434977629,"version":"3.56.0"},"reference-count":66,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFC1001301 81771651 81873832 82001561"],"award-info":[{"award-number":["2017YFC1001301 81771651 81873832 82001561"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>Although sperm preservation is a common means of personal fertility preservation, its effects on embryonic development potential need further investigation. The purpose of this study was to identify key microRNA (miRNA) in cryopreserved sperm and determine the changes of these miRNAs and their target genes during embryonic development using cryopreserved sperm. Moreover, the embryonic development potential of cryopreserved sperm was estimated in assisted reproductive technology (ART), where key miRNAs and target genes were validated in sperm and subsequent embryos. Clinical data of embryonic development from cryopreserved sperm indicated a significant decrease in fertilization rate in both <jats:italic>in vitro<\/jats:italic> fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cases, as well as a reduction in blastocyst formation rate in ICSI cases. Meanwhile there was a significant increase in blocked embryo ratio of Day1, Day2, and Day3.5 embryos when frozen-thawed mouse sperm was used, compared with fresh mouse sperm, suggesting a potential negative effect of sperm cryopreservation on embryonic development. From frozen-thawed and fresh sperm in humans and mice, respectively, 21 and 95 differentially expressed miRNAs (DEmiRs) were detected. miR-148b-3p were downregulated in both human and mouse frozen-thawed sperm and were also decreased in embryos after fertilization using cryopreserved sperm. Target genes of miR-148b-3p, Pten, was identified in mouse embryos using quantitative real-time PCR (qRT-PCR) and Western blot (WB). In addition, common characters of cryopreservation of mouse oocytes compared with sperm were also detected; downregulation of miR-148b-3p was also confirmed in cryopreserved oocytes. In summary, our study suggested that cryopreservation of sperm could change the expression of miRNAs, especially the miR-148b-3p across humans and mice, and may further affect fertilization and embryo development by increasing the expression of Pten. Moreover, downregulation of miR-148b-3p induced by cryopreservation was conserved in mouse gametes.<\/jats:p>","DOI":"10.3389\/fcell.2021.749486","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T08:46:00Z","timestamp":1640162760000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development"],"prefix":"10.3389","volume":"9","author":[{"given":"Xiaoyu","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqiong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yazhong","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingbin","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1016\/j.fertnstert.2019.12.028","article-title":"MicroRNAs in Combined Spent Culture media and Sperm Are Associated with Embryo Quality and Pregnancy Outcome","volume":"113","author":"Abu-Halima","year":"2020","journal-title":"Fertil. sterility"},{"key":"B2","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1016\/j.fertnstert.2013.11.020","article-title":"Thawed Human Sperm Quality Is Influenced by the Volume of the Cryopreserved Specimen","volume":"101","author":"Abush","year":"2014","journal-title":"Fertil. sterility"},{"key":"B3","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1093\/humupd\/dmz039","article-title":"The Role of microRNAs in Ovarian Function and the Transition toward Novel Therapeutic Strategies in Fertility Preservation: from Bench to Future Clinical Application","volume":"26","author":"Alexandri","year":"2020","journal-title":"Hum. Reprod. Update"},{"key":"B4","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1002\/bdr2.1599","article-title":"Does In Vitro Fertilization Affect the Expression of miRNAs and Their Biogenesis Pathway in Preimplantation Mouse Embryos?","volume":"112","author":"Azizi","year":"2020","journal-title":"Birth Defects Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1866","DOI":"10.1007\/s12094-021-02595-9","article-title":"Propofol Inhibited Gastric Cancer Proliferation via the Hsa-miR-328-3p\/STAT3 Pathway","volume":"23","author":"Bai","year":"2021","journal-title":"Clin. Transl Oncol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1093\/humupd\/dmz026","article-title":"Fertility Preservation for Transgender Adolescents and Young Adults: a Systematic Review","volume":"25","author":"Baram","year":"2019","journal-title":"Hum. Reprod. Update"},{"key":"B7","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1186\/s13148-020-00911-8","article-title":"What Impact Does Oocyte Vitrification Have on Epigenetics and Gene Expression?","volume":"12","author":"Barberet","year":"2020","journal-title":"Clin. Epigenet"},{"key":"B8","first-page":"1059","article-title":"A Review of WHO Laboratory Manual for the Examination and Processing of Human Semen 5th Edition","volume":"17","author":"Cao","year":"2011","journal-title":"Zhonghua Nan Ke Xue"},{"key":"B9","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1186\/s13046-018-0748-9","article-title":"SPIN1, Negatively Regulated by miR-148\/152, Enhances Adriamycin Resistance via Upregulating Drug Metabolizing Enzymes and Transporter in Breast Cancer","volume":"37","author":"Chen","year":"2018","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e14419","DOI":"10.1111\/ijcp.14419","article-title":"Systematic Review and Meta\u2010analysis for the Value of Thyroid Disorder Screening in Men with Ejaculatory Dysfunction","volume":"75","author":"Cihan","year":"2021","journal-title":"Int. J. Clin. Pract."},{"key":"B11","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1016\/j.fertnstert.2019.07.006","article-title":"Sperm microRNA Pairs: New Perspectives in the Search for Male Fertility Biomarkers","volume":"112","author":"Corral-Vazquez","year":"2019","journal-title":"Fertil. sterility"},{"key":"B12","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1038\/1235","article-title":"Pten Is Essential for Embryonic Development and Tumour Suppression","volume":"19","author":"Cristofano","year":"1998","journal-title":"Nat. Genet."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1893","DOI":"10.1093\/humrep\/13.7.1893","article-title":"Fertilization, Pregnancy and Embryo Implantation Rates after with Fresh or Frozen-Thawed Testicular Spermatozoa","volume":"13","author":"De Croo","year":"1998","journal-title":"Hum. Reprod."},{"key":"B14","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1016\/j.ydbio.2008.02.004","article-title":"Regulation of Cytosolic and Mitochondrial ATP Levels in Mouse Eggs and Zygotes","volume":"316","author":"Dumollard","year":"2008","journal-title":"Developmental Biol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most Mammalian mRNAs Are Conserved Targets of microRNAs","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/s10561-005-0275-8","article-title":"Cryopreservation and Sperm DNA Integrity","volume":"7","author":"Gandini","year":"2006","journal-title":"Cell Tissue Banking"},{"key":"B17","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1016\/j.bbagrm.2012.03.007","article-title":"Expression Dynamics of microRNA Biogenesis during Preimplantation Mouse Development","volume":"1819","author":"Garc\u00eda-L\u00f3pez","year":"2012","journal-title":"Biochim. Biophys. Acta (Bba) - Gene Regul. Mech."},{"key":"B18","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1007\/s00774-019-01055-3","article-title":"Intra-articular Injection of hUC-MSCs Expressing miR-140-5p Induces Cartilage Self-Repairing in the Rat Osteoarthritis","volume":"38","author":"Geng","year":"2020","journal-title":"J. Bone Miner Metab."},{"key":"B19","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1016\/j.fertnstert.2011.07.1087","article-title":"Self-correction in Tripronucleated Human Embryos","volume":"96","author":"Grau","year":"2011","journal-title":"Fertil. sterility"},{"key":"B20","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.fertnstert.2011.12.035","article-title":"Human Spermatozoal RNAs","volume":"97","author":"Hamatani","year":"2012","journal-title":"Fertil. sterility"},{"key":"B21","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1530\/rep.1.00664","article-title":"Expression of IGF-II mRNA-Binding Proteins (IMPs) in Gonads and Testicular Cancer","volume":"130","author":"Hammer","year":"2005","journal-title":"Reproduction (Cambridge, England)"},{"key":"B22","doi-asserted-by":"publisher","first-page":"3203","DOI":"10.3892\/etm.2020.8574","article-title":"miR-106b-5p Promotes Cell Proliferation and Cell Cycle Progression by Directly Targeting CDKN1A in Osteosarcoma","volume":"19","author":"He","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"B23","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1016\/s0015-0282(16)56296-7","article-title":"Cryopreservation of Human Spermatozoa. IV. The Effects of Cooling Rate and Warming Rate on the Maintenance of Motility, Plasma Membrane Integrity, and Mitochondrial function**Supported by grants from the National Institutes of Health, Bethesda, Maryland (R01-Hd25949 and K04-Hd00980); Parke-Davis; Methodist Hospital; and by the Office of Health and Environmental Research, United States Department of Energy, Washington, D.C., under Contract DE-AC05-840R21400 with Martin Marietta Energy Systems.\u2020\u2020Presented in Part at the Annual Meeting of the American Society of Andrology, New Orleans, Louisiana, April 13 to 16, 1989, and at the Annual Meeting of the American Society of Andrology, Tampa, Florida, April 17 to 19, 1993.\u2021\u2021The Submitted Manuscript Has Been Authored by a Contractor of the United States Government under Contract DE-AC05-840R21400. Accordingly, the United States Government Retains a Nonexclusive, Royalty-free License to Publish or Reproduce the Published Form of This Contribution, or Allow Others to Do So, for U.S. Government Purposes","volume":"60","author":"Henry","year":"1993","journal-title":"Fertil. sterility"},{"key":"B24","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.rbmo.2018.05.012","article-title":"Sperm Cryopreservation: A Review on Current Molecular Cryobiology and Advanced Approaches","volume":"37","author":"Hezavehei","year":"2018","journal-title":"Reprod. Biomed. Online"},{"key":"B25","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1002\/cbf.3585","article-title":"MiR \u2010140\u20105p Promotes Osteogenic Differentiation of Mouse Embryonic Bone Marrow Mesenchymal Stem Cells and post\u2010fracture Healing of Mice","volume":"38","author":"Jiao","year":"2020","journal-title":"Cell Biochem Funct"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1016\/j.fertnstert.2008.07.1776","article-title":"Fresh Testicular Sperm Retrieved from Men with Spinal Cord Injury Retains Equal Fecundity to that from Men with Obstructive Azoospermia via Intracytoplasmic Sperm Injection","volume":"92","author":"Kanto","year":"2009","journal-title":"Fertil. sterility"},{"key":"B27","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.reprotox.2017.03.005","article-title":"Altered Helper T Cell-Mediated Immune Responses in Male Mice Conceived through In Vitro Fertilization","volume":"69","author":"Karimi","year":"2017","journal-title":"Reprod. Toxicol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1089\/bio.2020.0121","article-title":"Effect of miR-30a-5p on Apoptosis, Colonization, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells","volume":"19","author":"Khanlari","year":"2021","journal-title":"Biopreservation and biobanking"},{"key":"B29","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1080\/15384101.2019.1693748","article-title":"Inactivation of Mdm2 Restores Apoptosis Proficiency of Cooperativity Mutant P53 In Vivo","volume":"19","author":"Klimovich","year":"2020","journal-title":"Cell Cycle"},{"key":"B30","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1093\/humupd\/dmy016","article-title":"Ethics of Oocyte Banking for Third-Party Assisted Reproduction: a Systematic Review","volume":"24","author":"Kool","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"B31","doi-asserted-by":"publisher","first-page":"D68","DOI":"10.1093\/nar\/gkt1181","article-title":"miRBase: Annotating High Confidence microRNAs Using Deep Sequencing Data","volume":"42","author":"Kozomara","year":"2014","journal-title":"Nucl. Acids Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1095\/biolreprod.114.123588","article-title":"MicroRNAome of Porcine Conceptuses and Trophoblasts: Expression Profile of microRNAs and Their Potential to Regulate Genes Crucial for Establishment of Pregnancy1","volume":"92","author":"Krawczynski","year":"2015","journal-title":"Biol. Reprod."},{"key":"B33","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1016\/j.fertnstert.2007.12.061","article-title":"Poor Sperm Quality Affects Clinical Outcomes of Intracytoplasmic Sperm Injection in Fresh and Subsequent Frozen-Thawed Cycles: Potential Paternal Effects on Pregnancy Outcomes","volume":"91","author":"Lee","year":"2009","journal-title":"Fertil. sterility"},{"key":"B34","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1262\/jrd.2015-021","article-title":"Junction-mediating and Regulatory Protein (JMY) Is Essential for Early Porcine Embryonic Development","volume":"61","author":"Lin","year":"2015","journal-title":"J. Reprod. Development"},{"key":"B35","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1073\/pnas.1110368109","article-title":"Sperm-borne microRNA-34c Is Required for the First Cleavage Division in Mouse","volume":"109","author":"Liu","year":"2012","journal-title":"Proc. Natl. Acad. Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"687","DOI":"10.2527\/jas1983.563687x","article-title":"Fertility of Unfrozen and Frozen Stallion Spermatozoa Extended in EDTA-Lactose-Egg Yolk and Packaged in Straws","volume":"56","author":"Loomis","year":"1983","journal-title":"J. Anim. Sci."},{"key":"B37","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.exer.2018.07.003","article-title":"Molecular and Functional Characterization of Circulating Extracellular Vesicles from Diabetic Patients with and without Retinopathy and Healthy Subjects","volume":"176","author":"Mazzeo","year":"2018","journal-title":"Exp. Eye Res."},{"key":"B38","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1373\/clinchem.2010.157198","article-title":"Analysis of Circulating MicroRNA: Preanalytical and Analytical Challenges","volume":"57","author":"McDonald","year":"2011","journal-title":"Clin. Chem."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1016\/j.fertnstert.2013.08.054","article-title":"Clinical Guidelines for Sperm Cryopreservation in Cancer Patients","volume":"100","author":"Nangia","year":"2013","journal-title":"Fertil. sterility"},{"key":"B40","doi-asserted-by":"publisher","first-page":"BSR20190150","DOI":"10.1042\/bsr20190150","article-title":"miR-140-5p Induces Cell Apoptosis and Decreases Warburg Effect in Chronic Myeloid Leukemia by Targeting SIX1","volume":"39","author":"Nie","year":"2019","journal-title":"Biosci. Rep."},{"key":"B41","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/j.molmed.2021.01.005","article-title":"Delaying Reproductive Aging by Ovarian Tissue Cryopreservation and Transplantation: Is it Prime Time?","volume":"27","author":"Oktay","year":"2021","journal-title":"Trends Molecular Medicine"},{"key":"B42","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1261\/rna.046482.114","article-title":"The Small RNA Content of Human Sperm Reveals Pseudogene-Derived piRNAs Complementary to Protein-Coding Genes","volume":"21","author":"Pantano","year":"2015","journal-title":"Rna"},{"key":"B43","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1385\/0-89603-358-9:291","article-title":"Primerselect","volume":"70","author":"Plasterer","year":"1997","journal-title":"Methods Mol. Biol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"e201910041","DOI":"10.1083\/jcb.201910041","article-title":"PTEN Dephosphorylates Abi1 to Promote Epithelial Morphogenesis","volume":"219","author":"Qi","year":"2020","journal-title":"J. Cel. Biol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1016\/j.biopha.2017.02.062","article-title":"MiR-30b-5p Acts as a Tumor Suppressor, Repressing Cell Proliferation and Cell Cycle in Human Hepatocellular Carcinoma","volume":"89","author":"Qin","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"B46","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/j.fertnstert.2013.05.020","article-title":"Dead Spermatozoa in Raw Semen Samples Impair In Vitro Fertilization Outcomes of Frozen-Thawed Spermatozoa","volume":"100","author":"Roca","year":"2013","journal-title":"Fertil. sterility"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1411","DOI":"10.1038\/ncb1987","article-title":"The Let-7 Target Gene Mouse Lin-41 Is a Stem Cell Specific E3 Ubiquitin Ligase for the miRNA Pathway Protein Ago2","volume":"11","author":"Rybak","year":"2009","journal-title":"Nat. Cel Biol"},{"key":"B48","first-page":"136","article-title":"Use of Frozen-Thawed Oocytes for Efficient Production of normal Offspring from Cryopreserved Mouse Spermatozoa Showing Low Fertility","volume":"55","author":"Sakamoto","year":"2005","journal-title":"Comp. Med."},{"key":"B49","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1111\/rda.13897","article-title":"Proteomic Profile of Pre\u2010implantational Ovine Embryos Produced In Vivo","volume":"56","author":"Sanchez","year":"2021","journal-title":"Reprod. Dom Anim."},{"key":"B50","doi-asserted-by":"publisher","first-page":"e21440","DOI":"10.1096\/fj.202002448R","article-title":"Insulin Signaling Is an Essential Regulator of Endometrial Proliferation and Implantation in Mice","volume":"35","author":"Sekulovski","year":"2021","journal-title":"FASEB j."},{"key":"B51","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1007\/s13402-021-00615-y","article-title":"Correction to: Downregulated Exosomal microRNA-148b-3p in Cancer Associated Fibroblasts Enhance Chemosensitivity of Bladder Cancer Cells by Downregulating the Wnt\/\u03b2-Catenin Pathway and Upregulating PTEN","volume":"44","author":"Shan","year":"2021","journal-title":"Cell Oncol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"117895","DOI":"10.1016\/j.lfs.2020.117895","article-title":"The Effect of Sperm miR-34c on Human Embryonic Development Kinetics and Clinical Outcomes","volume":"256","author":"Shi","year":"","journal-title":"Life Sci."},{"key":"B53","doi-asserted-by":"publisher","first-page":"11045","DOI":"10.2147\/ott.S260499","article-title":"Hsa-MiR-590-3p Promotes the Malignancy Progression of Pancreatic Ductal Carcinoma by Inhibiting the Expression of P27 and PPP2R2A via G1\/S Cell Cycle Pathway","volume":"13","author":"Shi","year":"","journal-title":"Ott"},{"key":"B54","doi-asserted-by":"publisher","first-page":"3025","DOI":"10.1158\/1078-0432.Ccr-16-2304","article-title":"An miRNA-DNMT1 Axis Is Involved in Azacitidine Resistance and Predicts Survival in Higher-Risk Myelodysplastic Syndrome and Low Blast Count Acute Myeloid Leukemia","volume":"23","author":"Solly","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"B55","first-page":"471","article-title":"Bias in Meta-Analysis Detected by a Simple, Graphical Test. Increase in Studies of Publication Bias Coincided with Increasing Use of Meta-Analysis","volume":"316","author":"Song","year":"1998","journal-title":"BMJ"},{"key":"B56","doi-asserted-by":"publisher","first-page":"4254","DOI":"10.1182\/blood.V110.11.4254.4254","article-title":"Molecular Remission in MLL\/AF4-positive Infant Leukemia Treated with the All Trans-retinoic Acid Based MLL-Baby Protocol","volume":"110","author":"Tsaur","year":"2007","journal-title":"Blood"},{"key":"B57","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.cryobiol.2020.11.012","article-title":"Effect of Sulforaphane on Apoptosis, Reactive Oxygen Species and Lipids Peroxidation of Human Sperm during Cryopreservation","volume":"99","author":"Valipour","year":"2021","journal-title":"Cryobiology"},{"key":"B58","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1159\/000485650","article-title":"MiR-106b-5p Promotes Proliferation and Inhibits Apoptosis by Regulating BTG3 in Non-small Cell Lung Cancer","volume":"44","author":"Wei","year":"2017","journal-title":"Cell Physiol Biochem"},{"key":"B59","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1186\/s12864-020-07206-5","article-title":"Sperm miRNAs- Potential Mediators of Bull Age and Early Embryo Development","volume":"21","author":"Wu","year":"2020","journal-title":"BMC genomics"},{"key":"B60","doi-asserted-by":"publisher","first-page":"e110163","DOI":"10.1371\/journal.pone.0110163","article-title":"Integrated microRNA and mRNA Transcriptome Sequencing Reveals the Potential Roles of miRNAs in Stage I Endometrioid Endometrial Carcinoma","volume":"9","author":"Xiong","year":"2014","journal-title":"PloS one"},{"key":"B61","doi-asserted-by":"publisher","first-page":"e1501482","DOI":"10.1126\/sciadv.1501482","article-title":"Highly Sensitive Sequencing Reveals Dynamic Modifications and Activities of Small RNAs in Mouse Oocytes and Early Embryos","volume":"2","author":"Yang","year":"2016","journal-title":"Sci. Adv."},{"key":"B62","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.cr.2019.10.001","article-title":"The First Cell Fate Decision in Pre-implantation Mouse Embryos","volume":"8","author":"Yao","year":"2019","journal-title":"Cel Regen."},{"key":"B63","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1007\/s10815-018-1206-5","article-title":"Comparison of Intracytoplasmic Sperm Injection Outcome with Fresh versus Frozen-Thawed Testicular Sperm in Men with Nonobstructive Azoospermia: a Systematic Review and Meta-Analysis","volume":"35","author":"Yu","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"B64","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1242\/bio.201410959","article-title":"mir-34b\/c and Mir-449a\/b\/c Are Required for Spermatogenesis, but Not for the First Cleavage Division in Mice","volume":"4","author":"Yuan","year":"2015","journal-title":"Biol. open"},{"key":"B65","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.15585\/mmwr.mm6944e3","article-title":"Update: Characteristics of Symptomatic Women of Reproductive Age with Laboratory-Confirmed SARS-CoV-2 Infection by Pregnancy Status - United States, January 22-October 3, 2020","volume":"69","author":"Zambrano","year":"2020","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"B66","doi-asserted-by":"publisher","first-page":"145113","DOI":"10.1016\/j.gene.2020.145113","article-title":"Circular RNA Circ_0000442 Acts as a Sponge of MiR-148b-3p to Suppress Breast Cancer via PTEN\/PI3K\/Akt Signaling Pathway","volume":"766","author":"Zhang","year":"2021","journal-title":"Gene"}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2021.749486\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T08:46:08Z","timestamp":1640162768000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2021.749486\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,22]]},"references-count":66,"alternative-id":["10.3389\/fcell.2021.749486"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2021.749486","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,22]]},"article-number":"749486"}}