{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:26:12Z","timestamp":1784827572350,"version":"3.55.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970809 81870679 81570859"],"award-info":[{"award-number":["81970809 81870679 81570859"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>Long non-coding RNAs (lncRNAs) have been widely implicated in human diseases. Our aim was to explore the regulatory role of changes in the expression levels of PNKY and its linked signaling networks in mediating stress-induced choroidal neovascularization. PNKY expression levels were reduced in mice by laser and exposure of endothelial cell to hypoxic stress. PNKY silencing exacerbated the formation of CNV in a laser-induced CNV model and an <jats:italic>ex vivo<\/jats:italic> model, while overexpression inhibited CNV development. Silencing or overexpression of PNKY altered the viability, proliferation, migration, and tube-forming capacity of endothelial cells <jats:italic>in vitro<\/jats:italic>. Mechanistically, through the lncRNA\u2013RNA binding protein\u2013miRNA interaction analysis involving loss of function and gain-of-function experiments, we found that lncRNA PNKY inhibited the binding of miR124 to PTBP1 and maintained the homeostasis of choroidal vascular function by promoting Bcl-2 like protein 11 (BIM), and its dysfunction led to exacerbation of CNV lesion. Therefore, this study suggests that the lncPNKY\/PTBP1\u2013miR-124 axis is involved in regulating the development of CNV, providing a potential therapeutic target for the treatment of CNV.<\/jats:p>","DOI":"10.3389\/fcell.2022.836031","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T11:17:39Z","timestamp":1645442259000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Long Non-Coding RNA PNKY Modulates the Development of Choroidal Neovascularization"],"prefix":"10.3389","volume":"10","author":[{"given":"Lianjun","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1016\/j.it.2019.04.001","article-title":"Long Noncoding RNAs in Host-Pathogen Interactions","volume":"40","author":"Agliano","year":"2019","journal-title":"Trends Immunol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e13235","DOI":"10.1111\/acel.13235","article-title":"Targeting miR\u2010124\/Ferroportin Signaling Ameliorated Neuronal Cell Death through Inhibiting Apoptosis and Ferroptosis in Aged Intracerebral Hemorrhage Murine Model","volume":"19","author":"Bao","year":"2020","journal-title":"Aging Cell"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1298","DOI":"10.1016\/j.cell.2013.02.012","article-title":"Long Noncoding RNAs: Cellular Address Codes in Development and Disease","volume":"152","author":"Batista","year":"2013","journal-title":"Cell"},{"key":"B4","doi-asserted-by":"publisher","first-page":"93","DOI":"10.3389\/fgene.2020.00093","article-title":"The Oncogenic and Tumor Suppressive Functions of the Long Noncoding RNA MALAT1: An Emerging Controversy","volume":"11","author":"Chen","year":"2020","journal-title":"Front. 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