{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:07:41Z","timestamp":1782835661634,"version":"3.54.5"},"reference-count":183,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T00:00:00Z","timestamp":1663027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>Trabecular meshwork (TM) tissue is subjected to constant mechanical stress due to the ocular pulse created by the cardiac cycle. This brings about alterations in the membrane lipids and associated cell\u2013cell adhesion and cell\u2013extracellular matrix (ECM) interactions, triggering intracellular signaling responses to counter mechanical insults. A loss of such response can lead to elevated intraocular pressure (IOP), a major risk factor for primary open-angle glaucoma. This study is aimed to understand the changes in signaling responses by TM subjected to mechanical stretch. We utilized multiomics to perform an unbiased mRNA sequencing to identify changes in transcripts, mass spectrometry- (MS-) based quantitative proteomics for protein changes, and multiple reaction monitoring (MRM) profiling-based MS and high-performance liquid chromatography (HPLC-) based MS to characterize the lipid changes. We performed pathway analysis to obtain an integrated map of TM response to mechanical stretch. The human TM cells subjected to mechanical stretch demonstrated an upregulation of protein quality control, oxidative damage response, pro-autophagic signal, induction of anti-apoptotic, and survival signaling. We propose that mechanical stretch-induced lipid signaling <jats:italic>via<\/jats:italic> increased ceramide and sphingomyelin potentially contributes to increased TM stiffness through actin-cytoskeleton reorganization and profibrotic response. Interestingly, increased phospholipids and diacylglycerol due to mechanical stretch potentially enable cell membrane remodeling and changes in signaling pathways to alter cellular contractility. Overall, we propose the mechanistic interplay of macromolecules to bring about a concerted cellular response in TM cells to achieve mechanotransduction and IOP regulation when TM cells undergo mechanical stretch.<\/jats:p>","DOI":"10.3389\/fcell.2022.874828","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T06:55:36Z","timestamp":1663052136000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":27,"title":["Multiomics analysis reveals the mechanical stress-dependent changes in trabecular meshwork cytoskeletal-extracellular matrix interactions"],"prefix":"10.3389","volume":"10","author":[{"given":"Avinash","family":"Soundararajan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha","family":"Sundararajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruna","family":"Wijeratne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Mosley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faith Christine","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjoy","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Padmanabhan Paranji","family":"Pattabiraman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1159\/000075925","article-title":"Cellular lipid metabolism and the role of lipids in progressive renal disease","volume":"24","author":"Abrass","year":"2004","journal-title":"Am. J. Nephrol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/j.exer.2008.01.013","article-title":"Extracellular matrix in the trabecular meshwork","volume":"86","author":"Acott","year":"2008","journal-title":"Exp. Eye Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"100897","DOI":"10.1016\/j.preteyeres.2020.100897","article-title":"Normal and glaucomatous outflow regulation","volume":"82","author":"Acott","year":"2021","journal-title":"Prog. Retin. Eye Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"e11686","DOI":"10.7759\/cureus.11686","article-title":"Epidemiology of glaucoma: The past, present, and predictions for the future","volume":"12","author":"Allison","year":"2020","journal-title":"Cureus"},{"key":"B5","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1002\/jbt.20212","article-title":"Phosphorylation of Nrf2 in the transcription activation domain by casein kinase 2 (CK2) is critical for the nuclear translocation and transcription activation function of Nrf2 in IMR-32 neuroblastoma cells","volume":"22","author":"Apopa","year":"2008","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"10202","DOI":"10.1038\/s41598-020-67087-2","article-title":"SB772077B (SB77) alleviated the aqueous outflow resistance mediated by cyclic mechanical stress in perfused human cadaveric eyes","volume":"10","author":"Ashwinbalaji","year":"2020","journal-title":"Sci. Rep."},{"key":"B7","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1167\/iovs.08-2508","article-title":"Intraocular pressure increases in parallel with systemic blood pressure during isometric exercise","volume":"50","author":"Bakke","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B8","doi-asserted-by":"publisher","first-page":"3435","DOI":"10.1074\/mcp.m116.060509","article-title":"TNIP2 is a hub protein in the NF-\u03baB network with both protein and RNA mediated interactions","volume":"15","author":"Banks","year":"2016","journal-title":"Mol. Cell. Proteomics"},{"key":"B9","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1038\/nature09204","article-title":"Network organization of the human autophagy system","volume":"466","author":"Behrends","year":"2010","journal-title":"Nature"},{"key":"B10","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: A practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc. Ser. B Methodol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1080\/15548627.2019.1581009","article-title":"Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs","volume":"15","author":"Birgisdottir","year":"2019","journal-title":"Autophagy"},{"key":"B12","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/s1350-9462(03)00018-1","article-title":"Gene expression in the trabecular meshwork and the influence of intraocular pressure","volume":"22","author":"Borras","year":"2003","journal-title":"Prog. Retin. Eye Res."},{"key":"B14","first-page":"1505","article-title":"Effects of mechanical stretching on trabecular matrix metalloproteinases","volume":"42","author":"Bradley","year":"2001","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B15","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1093\/bioinformatics\/bts731","article-title":"NGSUtils: A software suite for analyzing and manipulating next-generation sequencing datasets","volume":"29","author":"Breese","year":"2013","journal-title":"Bioinformatics"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.3390\/cancers6031597","article-title":"DNA mismatch repair and oxidative DNA damage: Implications for cancer biology and treatment","volume":"6","author":"Bridge","year":"2014","journal-title":"Cancers"},{"key":"B17","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.3390\/cells11071209","article-title":"Extracellular lipids in the lung and their role in pulmonary fibrosis","volume":"11","author":"Burgy","year":"2022","journal-title":"Cells"},{"key":"B18","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.tibs.2006.11.004","article-title":"Diacylglycerol, when simplicity becomes complex","volume":"32","author":"Carrasco","year":"2007","journal-title":"Trends Biochem. Sci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.yexcr.2017.06.003","article-title":"Endothelial cell-cell adhesion and signaling","volume":"358","author":"Cerutti","year":"2017","journal-title":"Exp. Cell. Res."},{"key":"B20","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1097\/01.smj.0000145389.15201.7f","article-title":"Relation between intraocular pressure and systemic health parameters in Taiwan","volume":"98","author":"Chen","year":"2005","journal-title":"South. Med. J."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1039\/c5sc03826j","article-title":"Systematic study of the dynamics and half-lives of newly synthesized proteins in human cells","volume":"7","author":"Chen","year":"2016","journal-title":"Chem. Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1038\/ng.3078","article-title":"Common variants near ABCA1 and in PMM2 are associated with primary open-angle glaucoma","volume":"46","author":"Chen","year":"2014","journal-title":"Nat. Genet."},{"key":"B23","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1167\/iovs.04-0120","article-title":"Role of aldehyde dehydrogenase isozymes in the defense of rat lens and human lens epithelial cells against oxidative stress","volume":"46","author":"Choudhary","year":"2005","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B24","volume-title":"Lipid profiles of Pacific green and hawksbill sea turtle plasma","author":"Clyde-Brockway","year":"2021"},{"key":"B25","doi-asserted-by":"publisher","first-page":"6359","DOI":"10.1021\/cr100404w","article-title":"Mammalian triacylglycerol metabolism: Synthesis, lipolysis, and signaling","volume":"111","author":"Coleman","year":"2011","journal-title":"Chem. Rev."},{"key":"B26","doi-asserted-by":"publisher","first-page":"210","DOI":"10.3892\/etm.2017.5353","article-title":"Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells","volume":"15","author":"Cong","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"B27","doi-asserted-by":"publisher","first-page":"R33","DOI":"10.1016\/j.cub.2012.10.041","article-title":"The role of lipids in the control of autophagy","volume":"23","author":"Dall'Armi","year":"2013","journal-title":"Curr. Biol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"2716","DOI":"10.1242\/jcs.043794","article-title":"Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton","volume":"122","author":"Dawe","year":"2009","journal-title":"J. Cell. Sci."},{"key":"B29","doi-asserted-by":"publisher","first-page":"304","DOI":"10.3389\/fgene.2013.00304","article-title":"The role of SNP-loop diuretic interactions in hypertension across ethnic groups in HyperGEN","volume":"4","author":"de Las Fuentes","year":"2013","journal-title":"Front. Genet."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1002\/jms.4301","article-title":"Comprehensive lipid profiling of early stage oocytes and embryos by MRM profiling","volume":"53","author":"de Lima","year":"2018","journal-title":"J. Mass Spectrom."},{"key":"B31","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s12551-016-0244-4","article-title":"The keap1-nrf2 pathway: Promising therapeutic target to counteract ROS-mediated damage in cancers and neurodegenerative diseases","volume":"9","author":"Deshmukh","year":"2017","journal-title":"Biophys. Rev."},{"key":"B32","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1186\/s12958-019-0487-6","article-title":"Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue","volume":"17","author":"Dipali","year":"2019","journal-title":"Reprod. Biol. Endocrinol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"Star: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"B34","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1159\/000487170","article-title":"Functional implications of cross-linked actin networks in trabecular meshwork cells","volume":"45","author":"Duffy","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"B35","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1373\/clinchem.2010.158766","article-title":"Proposed serum cholesterol reference measurement procedure by gas chromatography-isotope dilution mass spectrometry","volume":"57","author":"Edwards","year":"2011","journal-title":"Clin. Chem."},{"key":"B36","doi-asserted-by":"publisher","first-page":"37127","DOI":"10.1038\/srep37127","article-title":"Caveolin-1 modulates intraocular pressure: Implications for caveolae mechanoprotection in glaucoma","volume":"6","author":"Elliott","year":"2016","journal-title":"Sci. Rep."},{"key":"B37","doi-asserted-by":"publisher","first-page":"S311","DOI":"10.1194\/jlr.r800049-jlr200","article-title":"Membrane phospholipid synthesis and endoplasmic reticulum function","volume":"50","author":"Fagone","year":"2009","journal-title":"J. Lipid Res."},{"key":"B38","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1016\/j.devcel.2009.12.020","article-title":"Morgana\/chp-1, a ROCK inhibitor involved in centrosome duplication and tumorigenesis","volume":"18","author":"Ferretti","year":"2010","journal-title":"Dev. Cell."},{"key":"B39","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1111\/j.0959-9673.2004.00377.x","article-title":"Smad3 as a mediator of the fibrotic response","volume":"85","author":"Flanders","year":"2004","journal-title":"Int. J. Exp. Pathol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1038\/nchembio.392","article-title":"An update on sphingosine-1-phosphate and other sphingolipid mediators","volume":"6","author":"Fyrst","year":"2010","journal-title":"Nat. Chem. Biol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1167\/iovs.61.14.21","article-title":"Reduced pulsatile trabecular meshwork motion in eyes with primary open angle glaucoma using phase-sensitive optical coherence tomography","volume":"61","author":"Gao","year":"2020","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B42","doi-asserted-by":"publisher","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: A graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1038\/s41467-020-20851-4","article-title":"Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries","volume":"12","author":"Gharahkhani","year":"2021","journal-title":"Nat. Commun."},{"key":"B44","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1002\/jcp.22783","article-title":"PAI-1 in tissue fibrosis","volume":"227","author":"Ghosh","year":"2012","journal-title":"J. Cell. Physiol."},{"key":"B45","first-page":"653","article-title":"Microtubule disruption leads to cellular contraction in human trabecular meshwork cells","volume":"39","author":"Gills","year":"1998","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B46","first-page":"352","article-title":"Genes upregulated in the human trabecular meshwork in response to elevated intraocular pressure","volume":"41","author":"Gonzalez","year":"2000","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B47","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1038\/sj.cdd.4401369","article-title":"hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria","volume":"11","author":"Gotoh","year":"2004","journal-title":"Cell. Death Differ."},{"key":"B48","doi-asserted-by":"publisher","first-page":"11717","DOI":"10.1073\/pnas.1120068109","article-title":"Wnt pathway inhibition via the targeting of Frizzled receptors results in decreased growth and tumorigenicity of human tumors","volume":"109","author":"Gurney","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B49","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/s40135-015-0072-x","article-title":"Recent developments in understanding the role of aqueous humor outflow in normal and primary open angle glaucoma","volume":"3","author":"Hann","year":"2015","journal-title":"Curr. Ophthalmol. Rep."},{"key":"B50","doi-asserted-by":"publisher","first-page":"107920","DOI":"10.1016\/j.exer.2020.107920","article-title":"Nuclear factor-kappa beta signaling is required for transforming growth factor Beta-2 induced ocular hypertension","volume":"191","author":"Hernandez","year":"2020","journal-title":"Exp. Eye Res."},{"key":"B51","doi-asserted-by":"publisher","first-page":"165560","DOI":"10.1016\/j.bbadis.2019.165560","article-title":"Role of the autotaxin-lysophosphatidic acid axis in glaucoma, aqueous humor drainage and fibrogenic activity","volume":"1866","author":"Ho","year":"2020","journal-title":"Biochimica Biophysica Acta (BBA) - Mol. Basis Dis."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1969","DOI":"10.1167\/iovs.17-23702","article-title":"Lysophosphatidic acid induces ECM production via activation of the mechanosensitive YAP\/TAZ transcriptional pathway in trabecular meshwork cells","volume":"59","author":"Ho","year":"2018","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B53","doi-asserted-by":"publisher","first-page":"e52507","DOI":"10.15252\/embr.202152507","article-title":"Maintaining proteostasis under mechanical stress","volume":"22","author":"H\u00f6hfeld","year":"2021","journal-title":"EMBO Rep."},{"key":"B54","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1167\/iovs.17-23218","article-title":"Autotaxin-lysophosphatidic acid pathway in intraocular pressure regulation and glaucoma subtypes","volume":"59","author":"Honjo","year":"2018","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B55","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/bs.ctm.2019.07.001","article-title":"Structural and signaling role of lipids in plasma membrane repair","volume":"84","author":"Horn","year":"2019","journal-title":"Curr. Top. Membr."},{"key":"B56","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1007\/s10545-010-9061-2","article-title":"A general introduction to the biochemistry of mitochondrial fatty acid \u03b2\u2010oxidation","volume":"33","author":"Houten","year":"2010","journal-title":"J Inher Metab Disea"},{"key":"B57","doi-asserted-by":"publisher","first-page":"e0151833","DOI":"10.1371\/journal.pone.0151833","article-title":"Increased intraocular pressure and hyperglycemic level in diabetic patients","volume":"11","author":"Hymowitz","year":"2016","journal-title":"PLOS ONE"},{"key":"B58","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1038\/nm1118","article-title":"Salt-sensitive hypertension is triggered by Ca2+ entry via Na+\/Ca2+ exchanger type-1 in vascular smooth muscle","volume":"10","author":"Iwamoto","year":"2004","journal-title":"Nat. Med."},{"key":"B59","doi-asserted-by":"publisher","first-page":"e42627","DOI":"10.1371\/journal.pone.0042627","article-title":"Autotaxin-lysophosphatidic acid axis is a novel molecular target for lowering intraocular pressure","volume":"7","author":"Iyer","year":"2012","journal-title":"PLoS One"},{"key":"B60","doi-asserted-by":"publisher","first-page":"3411","DOI":"10.1038\/s41467-018-05646-y","article-title":"The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates","volume":"9","author":"Jakobsson","year":"2018","journal-title":"Nat. Commun."},{"key":"B61","doi-asserted-by":"publisher","first-page":"8239","DOI":"10.1093\/nar\/gkx432","article-title":"Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation","volume":"45","author":"Jakobsson","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"B62","first-page":"2577","article-title":"Aqueous humor and the dynamics of its flow","author":"Johnson","year":"2000","journal-title":"Princ. Pract. Ophthalmol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1097\/01.ijg.0000131757.63542.24","article-title":"The aqueous outflow system as a mechanical pump","volume":"13","author":"Johnstone","year":"","journal-title":"J. glaucoma"},{"key":"B64","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1097\/01.ijg.0000131757.63542.24","article-title":"The aqueous outflow system as a mechanical pump","volume":"13","author":"Johnstone","year":"","journal-title":"J. Glaucoma"},{"key":"B65","doi-asserted-by":"publisher","first-page":"100917","DOI":"10.1016\/j.preteyeres.2020.100917","article-title":"Aqueous outflow regulation - 21st century concepts","volume":"83","author":"Johnstone","year":"2021","journal-title":"Prog. Retin. Eye Res."},{"key":"B66","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1016\/0002-9394(89)90266-3","article-title":"Neural crest origin of trabecular meshwork cells and other structures of the anterior chamber","volume":"107","author":"Kaiser-Kupfer","year":"1989","journal-title":"Am. J. Ophthalmol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.molcel.2004.08.008","article-title":"TAB2 and TAB3 activate the NF-\u03baB pathway through binding to polyubiquitin chains","volume":"15","author":"Kanayama","year":"2004","journal-title":"Mol. Cell."},{"key":"B68","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1016\/j.bbalip.2007.04.003","article-title":"Structure and function of phosphatidylcholine transfer protein (PC-TP)\/StarD2","volume":"1771","author":"Kanno","year":"2007","journal-title":"Biochimica Biophysica Acta (BBA) - Mol. Cell. Biol. Lipids"},{"key":"B69","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.exer.2018.03.001","article-title":"Consensus recommendations for trabecular meshwork cell isolation, characterization and culture","volume":"171","author":"Keller","year":"2018","journal-title":"Exp. Eye Res."},{"key":"B70","doi-asserted-by":"publisher","first-page":"1164","DOI":"10.1167\/iovs.06-0875","article-title":"Extracellular matrix gene alternative splicing by trabecular meshwork cells in response to mechanical stretching","volume":"48","author":"Keller","year":"2007","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B71","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/s0014-4835(02)00255-5","article-title":"The role of protein kinase C in modulation of aqueous humor outflow facility","volume":"76","author":"Khurana","year":"2003","journal-title":"Exp. Eye Res."},{"key":"B72","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1101\/gad.14.9.1085","article-title":"MutS homolog 4 localization to meiotic chromosomes is required for chromosome pairing during meiosis in male and female mice","volume":"14","author":"Kneitz","year":"2000","journal-title":"Genes. Dev."},{"key":"B73","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1002\/iub.1865","article-title":"Regulation of microtubule stability by centrosomal proteins","volume":"70","author":"Kumari","year":"2018","journal-title":"IUBMB Life"},{"key":"B74","doi-asserted-by":"publisher","first-page":"100145","DOI":"10.1016\/j.jlr.2021.100145","article-title":"Membrane cholesterol regulates TRPV4 function, cytoskeletal expression, and the cellular response to tension","volume":"62","author":"Lakk","year":"2021","journal-title":"J. lipid Res."},{"key":"B75","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1167\/iovs.10-6342","article-title":"Elastic modulus determination of normal and glaucomatous human trabecular meshwork","volume":"52","author":"Last","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B76","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1101\/gad.236349.113","article-title":"Insights into the biological functions of Dock family guanine nucleotide exchange factors","volume":"28","author":"Laurin","year":"2014","journal-title":"Genes. Dev."},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005918","article-title":"Protein regulation in signal transduction","volume":"8","author":"Lee","year":"2016","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1006\/excr.1996.0252","article-title":"Structural analysis of a human glial variant laminin","volume":"227","author":"LeMosy","year":"1996","journal-title":"Exp. Cell. Res."},{"key":"B79","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1042\/bst0390495","article-title":"Regulation of the SREBP transcription factors by mTORC1","volume":"39","author":"Lewis","year":"2011","journal-title":"Biochem. Soc. Trans."},{"key":"B80","doi-asserted-by":"publisher","first-page":"3369","DOI":"10.3892\/or.2017.5577","article-title":"TXNIP overexpression suppresses proliferation and induces apoptosis in SMMC7221 cells through ROS generation and MAPK pathway activation","volume":"37","author":"Li","year":"","journal-title":"Oncol. Rep."},{"key":"B81","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.7150\/ijms.20653","article-title":"HSPB8 promotes the fusion of autophagosome and lysosome during autophagy in diabetic neurons","volume":"14","author":"Li","year":"","journal-title":"Int. J. Med. Sci."},{"key":"B82","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"B83","doi-asserted-by":"publisher","first-page":"1830","DOI":"10.1038\/s41467-017-01561-w","article-title":"Identification of a neural crest stem cell niche by Spatial Genomic Analysis","volume":"8","author":"Lignell","year":"2017","journal-title":"Nat. Commun."},{"key":"B84","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1002\/jcp.20404","article-title":"Induction of TGF-\u03b21 in the trabecular meshwork under cyclic mechanical stress","volume":"205","author":"Liton","year":"2005","journal-title":"J. Cell. Physiol."},{"key":"B85","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.survophthal.2017.06.007","article-title":"Aging and ocular tissue stiffness in glaucoma","volume":"63","author":"Liu","year":"2018","journal-title":"Surv. Ophthalmol."},{"key":"B86","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/0962-8924(96)20013-3","article-title":"pH, EF-1\u03b1 and the cytoskeleton","volume":"6","author":"Liu","year":"1996","journal-title":"Trends Cell. Biol."},{"key":"B87","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1038\/aps.2015.101","article-title":"ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro","volume":"36","author":"Liu","year":"2015","journal-title":"Acta Pharmacol. Sin."},{"key":"B88","doi-asserted-by":"publisher","first-page":"6382","DOI":"10.1167\/iovs.13-12128","article-title":"Gene expression profile in human trabecular meshwork from patients with primary open-angle glaucoma","volume":"54","author":"Liu","year":"2013","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B89","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1186\/s13023-020-01564-9","article-title":"Factor XII in PMM2-CDG patients: Role of N-glycosylation in the secretion and function of the first element of the contact pathway","volume":"15","author":"L\u00f3pez-G\u00e1lvez","year":"2020","journal-title":"Orphanet J. Rare Dis."},{"key":"B90","first-page":"534","article-title":"Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells","volume":"15","author":"Luna","year":"2009","journal-title":"Mol. Vis."},{"key":"B91","doi-asserted-by":"publisher","first-page":"2447","DOI":"10.1002\/jcp.25582","article-title":"Vertebrate lonesome kinase regulated extracellular matrix protein phosphorylation, cell shape, and adhesion in trabecular meshwork cells","volume":"232","author":"Maddala","year":"2017","journal-title":"J. Cell. Physiol."},{"key":"B92","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1194\/jlr.c066712","article-title":"Srebp2: A master regulator of sterol and fatty acid synthesis","volume":"57","author":"Madison","year":"2016","journal-title":"J. Lipid Res."},{"key":"B93","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1007\/s11745-008-3152-y","article-title":"Membrane lipid composition plays a central role in the maintenance of epithelial cell adhesion to the extracellular matrix","volume":"43","author":"M\u00e1rquez","year":"2008","journal-title":"Lipids"},{"key":"B94","doi-asserted-by":"publisher","first-page":"824","DOI":"10.3389\/fphys.2018.00824","article-title":"Cellular mechanotransduction: From tension to function","volume":"9","author":"Martino","year":"2018","journal-title":"Front. Physiol."},{"key":"B95","first-page":"289","article-title":"Bovine and porcine trabecular cells produce prostaglandin F2 alpha in response to cyclic mechanical stretching","volume":"40","author":"Matsuo","year":"1996","journal-title":"Jpn. J. Ophthalmol."},{"key":"B96","doi-asserted-by":"publisher","first-page":"4288","DOI":"10.1093\/nar\/gks042","article-title":"Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation","volume":"40","author":"McCarthy","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B97","doi-asserted-by":"publisher","first-page":"20649","DOI":"10.1074\/jbc.m109.011767","article-title":"Stress-dependent Daxx-CHIP interaction suppresses the p53 apoptotic program","volume":"284","author":"McDonough","year":"2009","journal-title":"J. Biol. Chem."},{"key":"B98","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1001\/archopht.122.6.822","article-title":"Statins and other cholesterol-lowering medications and the presenceof glaucoma","volume":"122","author":"McGwin","year":"2004","journal-title":"Arch. Ophthalmol."},{"key":"B99","first-page":"2361","article-title":"A longitudinal study of the relationship between intraocular and blood pressures","volume":"31","author":"McLeod","year":"1990","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B100","doi-asserted-by":"publisher","first-page":"2263","DOI":"10.1167\/iovs.03-0960","article-title":"Role of lysophospholipid growth factors in the modulation of aqueous humor outflow facility","volume":"45","author":"Mettu","year":"2004","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B101","doi-asserted-by":"publisher","first-page":"33702","DOI":"10.1038\/srep33702","article-title":"Cholesterol alters the dynamics of release in protein independent cell models for exocytosis","volume":"6","author":"Najafinobar","year":"2016","journal-title":"Sci. Rep."},{"key":"B102","doi-asserted-by":"publisher","first-page":"16092","DOI":"10.1038\/s41598-019-52627-2","article-title":"Transcriptome analysis reveals autophagy as regulator of TGF\u03b2\/Smad-induced fibrogenesis in trabecular meshwork cells","volume":"9","author":"Nettesheim","year":"2019","journal-title":"Sci. Rep."},{"key":"B103","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1091\/mbc.e15-12-0835","article-title":"NF\u03baB is a central regulator of protein quality control in response to protein aggregation stresses via autophagy modulation","volume":"27","author":"Nivon","year":"2016","journal-title":"MBoC"},{"key":"B104","doi-asserted-by":"publisher","first-page":"3811","DOI":"10.1073\/pnas.1315359111","article-title":"MURC\/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by \u03b11-adrenergic receptors","volume":"111","author":"Ogata","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B105","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/s0021-5155(97)00129-9","article-title":"Bovine trabecular cells produce TIMP-1 and MMP-2 in response to mechanical stretching","volume":"42","author":"Okada","year":"1998","journal-title":"Jpn. J. Ophthalmol."},{"key":"B106","doi-asserted-by":"publisher","first-page":"4117","DOI":"10.4161\/cc.9.20.13387","article-title":"Role of a novel coiled-coil domain-containing protein CCDC69 in regulating central spindle assembly","volume":"9","author":"Pal","year":"2010","journal-title":"Cell. Cycle"},{"key":"B107","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1007\/s12551-018-0481-9","article-title":"Multifunctional roles of tropomodulin-3 in regulating actin dynamics","volume":"10","author":"Parreno","year":"2018","journal-title":"Biophys. Rev."},{"key":"B108","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.exer.2015.05.001","article-title":"Elevated intraocular pressure induces Rho GTPase mediated contractile signaling in the trabecular meshwork","volume":"136","author":"Pattabiraman","year":"","journal-title":"Exp. Eye Res."},{"key":"B109","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1002\/jcp.24524","article-title":"Regulation of plasticity and fibrogenic activity of trabecular meshwork cells by Rho GTPase signaling","volume":"229","author":"Pattabiraman","year":"2014","journal-title":"J. Cell. Physiol."},{"key":"B110","doi-asserted-by":"publisher","first-page":"C749","DOI":"10.1152\/ajpcell.00317.2009","article-title":"Mechanistic basis of Rho GTPase-induced extracellular matrix synthesis in trabecular meshwork cells","volume":"298","author":"Pattabiraman","year":"2010","journal-title":"Am. J. Physiology-Cell Physiology"},{"key":"B111","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/j.ajpath.2014.10.023","article-title":"RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork","volume":"185","author":"Pattabiraman","year":"","journal-title":"Am. J. Pathology"},{"key":"B112","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.ejphar.2016.04.048","article-title":"The exit strategy: Pharmacological modulation of extracellular matrix production and deposition for better aqueous humor drainage","volume":"787","author":"Pattabiraman","year":"2016","journal-title":"Eur. J. Pharmacol."},{"key":"B113","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1194\/jlr.r046391","article-title":"Autotaxin: Structure-function and signaling","volume":"55","author":"Perrakis","year":"2014","journal-title":"J. Lipid Res."},{"key":"B114","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.cell.2011.06.034","article-title":"mTOR complex 1 regulates lipin 1 localization to control the SREBP pathway","volume":"146","author":"Peterson","year":"2011","journal-title":"Cell."},{"key":"B115","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1101\/gad.1428006","article-title":"The myocardin family of transcriptional coactivators: Versatile regulators of cell growth, migration, and myogenesis","volume":"20","author":"Pipes","year":"2006","journal-title":"Genes. Dev."},{"key":"B116","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1111\/aos.14769","article-title":"Cholesterol and glaucoma: A systematic review and meta-analysis","volume":"100","author":"Posch-Pertl","year":"2022","journal-title":"Acta Ophthalmol."},{"key":"B117","doi-asserted-by":"publisher","first-page":"1379","DOI":"10.1172\/jci76369","article-title":"Emerging biology of sphingosine-1-phosphate: Its role in pathogenesis and therapy","volume":"125","author":"Proia","year":"2015","journal-title":"J. Clin. Investig."},{"key":"B118","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1007\/s12192-016-0676-6","article-title":"The human HSP70 family of chaperones: Where do we stand?","volume":"21","author":"Radons","year":"2016","journal-title":"Cell. Stress Chaperones"},{"key":"B119","doi-asserted-by":"publisher","first-page":"3826","DOI":"10.1167\/iovs.08-2694","article-title":"Cyclic mechanical stress and trabecular meshwork cell contractility","volume":"50","author":"Ramos","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B120","doi-asserted-by":"publisher","first-page":"3027","DOI":"10.2147\/opth.s292218","article-title":"Switch to autophagy the key mechanism for trabecular meshwork death in severe glaucoma","author":"Rao","year":"2021","journal-title":"Opth"},{"key":"B121","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1089\/jop.2013.0194","article-title":"Bioactive lysophospholipids: Role in regulation of aqueous humor outflow and intraocular pressure in the context of pathobiology and therapy of glaucoma","volume":"30","author":"Rao","year":"2014","journal-title":"J. Ocular Pharmacol. Ther."},{"key":"B122","first-page":"52","article-title":"Paracellular route of aqueous outflow in the trabecular meshwork and canal of Schlemm. A freeze-fracture study of the endothelial junctions in the sclerocorneal angel of the macaque monkey eye","volume":"21","author":"Raviola","year":"1981","journal-title":"Investigative Ophthalmol. Vis. Sci."},{"key":"B123","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1167\/iovs.61.10.45","article-title":"Aqueous humor outflow requires active cellular metabolism in mice","volume":"61","author":"Reina-Torres","year":"2020","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B124","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"B125","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1016\/j.bbamcr.2010.07.004","article-title":"Absence of ataxin-3 leads to cytoskeletal disorganization and increased cell death","volume":"1803","author":"Rodrigues","year":"2010","journal-title":"Biochimica Biophysica Acta (BBA) - Mol. Cell. Res."},{"key":"B126","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.arr.2016.05.012","article-title":"From DNA damage to functional changes of the trabecular meshwork in aging and glaucoma","volume":"29","author":"Sacc\u00e0","year":"2016","journal-title":"Ageing Res. Rev."},{"key":"B127","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1016\/s0079-6123(08)01127-8","article-title":"Oxidative stress and glaucoma: Injury in the anterior segment of the eye","volume":"173","author":"Sacc\u00e0","year":"2008","journal-title":"Prog. Brain Res."},{"key":"B128","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1001\/archopht.123.4.458","article-title":"Oxidative DNA damage in the human trabecular meshwork","volume":"123","author":"Sacc\u00e0","year":"2005","journal-title":"Arch. Ophthalmol."},{"key":"B129","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00091","article-title":"Membrane transporters in a human genome-scale metabolic knowledgebase and their implications for disease","volume":"5","author":"Sahoo","year":"2014","journal-title":"Front. Physiology"},{"key":"B130","doi-asserted-by":"publisher","first-page":"2948","DOI":"10.1182\/blood-2003-01-0173","article-title":"DOCK2 regulates Rac activation and cytoskeletal reorganization through interaction with ELMO1","volume":"102","author":"Sanui","year":"2003","journal-title":"Blood"},{"key":"B131","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1006\/bbrc.1999.0797","article-title":"A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye","volume":"259","author":"Sato","year":"1999","journal-title":"Biochem. Biophysical Res. Commun."},{"key":"B132","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.1038\/nmat4396","article-title":"Microtubules self-repair in response to mechanical stress","volume":"14","author":"Schaedel","year":"2015","journal-title":"Nat. Mater"},{"key":"B133","doi-asserted-by":"publisher","first-page":"21427","DOI":"10.1074\/jbc.m800014200","article-title":"DKK1 antagonizes Wnt signaling without promotion of LRP6 internalization and degradation","volume":"283","author":"Sem\u00ebnov","year":"2008","journal-title":"J. Biol. Chem."},{"key":"B134","doi-asserted-by":"publisher","first-page":"3910","DOI":"10.1038\/sj.emboj.7601823","article-title":"Essential role for TAX1BP1 in the termination of TNF-\u03b1-, IL-1- and LPS-mediated NF-\u03baB and JNK signaling","volume":"26","author":"Shembade","year":"2007","journal-title":"Embo J."},{"key":"B135","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2021942118","article-title":"Primary cilia and the reciprocal activation of AKT and SMAD2\/3 regulate stretch-induced autophagy in trabecular meshwork cells","volume":"118","author":"Shim","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B136","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1080\/15548627.2019.1662584","article-title":"The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress","volume":"16","author":"Shim","year":"2020","journal-title":"Autophagy"},{"key":"B137","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.molcel.2004.06.040","article-title":"hMSH4-hMSH5 recognizes Holliday Junctions and forms a meiosis-specific sliding clamp that embraces homologous chromosomes","volume":"15","author":"Snowden","year":"2004","journal-title":"Mol. Cell."},{"key":"B138","doi-asserted-by":"publisher","first-page":"2424","DOI":"10.1167\/iovs.04-0776","article-title":"Effects of cholesterol-lowering statins on the aqueous humor outflow pathway","volume":"46","author":"Song","year":"2005","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B139","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1186\/s12944-020-01389-2","article-title":"Signaling and other functions of lipids in autophagy: A review","volume":"19","author":"Soto-Avellaneda","year":"2020","journal-title":"Lipids Health Dis."},{"key":"B140","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1016\/j.exer.2009.08.008","article-title":"Sphingosine-1-phosphate effects on the inner wall of Schlemm's canal and outflow facility in perfused human eyes","volume":"89","author":"Stamer","year":"2009","journal-title":"Exp. Eye Res."},{"key":"B141","doi-asserted-by":"publisher","first-page":"108046","DOI":"10.1016\/j.exer.2020.108046","article-title":"Isolation and Characterization of primary human trabecular meshwork cells from segmental flow regions: New tools for understanding segmental flow","volume":"197","author":"Staverosky","year":"2020","journal-title":"Exp. Eye Res."},{"key":"B142","doi-asserted-by":"publisher","first-page":"2074","DOI":"10.1016\/j.ophtha.2012.04.029","article-title":"The relationship between statin use and open-angle glaucoma","volume":"119","author":"Stein","year":"2012","journal-title":"Ophthalmology"},{"key":"B143","doi-asserted-by":"publisher","first-page":"1892","DOI":"10.1016\/j.yexcr.2008.02.022","article-title":"Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness","volume":"314","author":"Stewart-Hutchinson","year":"2008","journal-title":"Exp. Cell. Res."},{"key":"B144","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1159\/000027471","article-title":"Regulation of trabecular meshwork contractility","volume":"214","author":"Stumpff","year":"2000","journal-title":"Ophthalmologica"},{"key":"B145","doi-asserted-by":"publisher","first-page":"txaa212","DOI":"10.1093\/tas\/txaa212","article-title":"A standardized model to study effects of varying 24-h colostrum dose on postnatal growth and development","volume":"4","author":"Su\u00e1rez-Trujillo","year":"2020","journal-title":"Transl. Anim. Sci."},{"key":"B146","doi-asserted-by":"publisher","first-page":"C1164","DOI":"10.1152\/ajpcell.00437.2010","article-title":"S1P2 receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology","volume":"300","author":"Sumida","year":"2011","journal-title":"Am. J. Physiology-Cell Physiology"},{"key":"B147","doi-asserted-by":"publisher","first-page":"6633","DOI":"10.1167\/iovs.10-5391","article-title":"Sphingosine-1-phosphate enhancement of cortical actomyosin organization in cultured human Schlemm's canal endothelial cell monolayers","volume":"51","author":"Sumida","year":"2010","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B148","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1167\/tvst.4.2.1","article-title":"Why do people (still) go blind from glaucoma?","volume":"4","author":"Susanna","year":"2015","journal-title":"Trans. Vis. Sci. Tech."},{"key":"B149","doi-asserted-by":"publisher","first-page":"7522","DOI":"10.1074\/jbc.m607177200","article-title":"Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1","volume":"282","author":"Takeda","year":"2007","journal-title":"J. Biol. Chem."},{"key":"B150","doi-asserted-by":"publisher","first-page":"25792","DOI":"10.1038\/srep25792","article-title":"Association between systemic oxidative stress and visual field damage in open-angle glaucoma","volume":"6","author":"Tanito","year":"2016","journal-title":"Sci. Rep."},{"key":"B151","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1016\/j.ophtha.2014.05.013","article-title":"Global prevalence of glaucoma and projections of glaucoma burden through 2040","volume":"121","author":"Tham","year":"2014","journal-title":"Ophthalmology"},{"key":"B152","first-page":"3254","article-title":"The effects of protein kinase C on trabecular meshwork and ciliary muscle contractility","volume":"40","author":"Thieme","year":"1999","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B153","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1038\/ng.661","article-title":"Common variants near CAV1 and CAV2 are associated with primary open-angle glaucoma","volume":"42","author":"Thorleifsson","year":"2010","journal-title":"Nat. Genet."},{"key":"B154","first-page":"239","article-title":"H-7 increases trabecular facility and facility after ciliary muscle disinsertion in monkeys","volume":"40","author":"Tian","year":"1999","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B155","doi-asserted-by":"publisher","first-page":"521","DOI":"10.3389\/fgene.2018.00521","article-title":"Hotspots for initiation of meiotic recombination","volume":"9","author":"Tock","year":"2018","journal-title":"Front. Genet."},{"key":"B156","doi-asserted-by":"publisher","first-page":"26555","DOI":"10.1073\/pnas.1911837116","article-title":"Increased stiffness and flow resistance of the inner wall of Schlemm\u2019s canal in glaucomatous human eyes","volume":"116","author":"Vahabikashi","year":"2019","journal-title":"Proc. Natl. Acad. Sci."},{"key":"B157","doi-asserted-by":"publisher","first-page":"F29","DOI":"10.1083\/jcb.152.5.f29","article-title":"Caveolin, cholesterol, and lipid droplets?","volume":"152","author":"Van Meer","year":"2001","journal-title":"J. Cell. Biol."},{"key":"B158","doi-asserted-by":"publisher","first-page":"2857","DOI":"10.1167\/iovs.05-0075","article-title":"Changes in gene expression by trabecular meshwork cells in response to mechanical stretching","volume":"46","author":"Vittal","year":"2005","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B159","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1002\/jcp.20030","article-title":"Genes expressed in the human trabecular meshwork during pressure-induced homeostatic response","volume":"201","author":"Vittitow","year":"2004","journal-title":"J. Cell. Physiol."},{"key":"B160","doi-asserted-by":"publisher","first-page":"2816","DOI":"10.1167\/iovs.08-2466","article-title":"Increased RhoA and RhoB protein accumulation in cultured human trabecular meshwork cells by lovastatin","volume":"50","author":"Von Zee","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B161","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1146\/annurev-biochem-061009-102430","article-title":"Lipid droplets and cellular lipid metabolism","volume":"81","author":"Walther","year":"2012","journal-title":"Annu. Rev. Biochem."},{"key":"B162","doi-asserted-by":"publisher","first-page":"4809","DOI":"10.1167\/iovs.17-22175","article-title":"Estimating human trabecular meshwork stiffness by numerical modeling and advanced OCT imaging","volume":"58","author":"Wang","year":"2017","journal-title":"Investigative Ophthalmol. Vis. Sci."},{"key":"B163","doi-asserted-by":"publisher","first-page":"1028","DOI":"10.1167\/iovs.18-25845","article-title":"Hyperlipidemia, blood lipid level, and the risk of glaucoma: A meta-analysis","volume":"60","author":"Wang","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B164","doi-asserted-by":"publisher","first-page":"2373","DOI":"10.1016\/j.ophtha.2009.05.041","article-title":"Major eye diseases and risk factors associated with systemic hypertension in an adult Chinese population: The beijing eye study","volume":"116","author":"Wang","year":"2009","journal-title":"Ophthalmology"},{"key":"B165","doi-asserted-by":"publisher","first-page":"16067","DOI":"10.1038\/nrdp.2016.67","article-title":"Primary open-angle glaucoma","volume":"2","author":"Weinreb","year":"2016","journal-title":"Nat. Rev. Dis. Prim."},{"key":"B166","first-page":"1012","article-title":"Role of protein tyrosine kinase on regulation of trabecular meshwork and ciliary muscle contractility","volume":"39","author":"Wiederholt","year":"1998","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B167","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/s1350-9462(99)00015-4","article-title":"The regulation of trabecular meshwork and ciliary muscle contractility","volume":"19","author":"Wiederholt","year":"2000","journal-title":"Prog. Retin Eye Res."},{"key":"B168","doi-asserted-by":"publisher","first-page":"R21","DOI":"10.1093\/hmg\/ddx184","article-title":"Genetics of glaucoma","volume":"26","author":"Wiggs","year":"2017","journal-title":"Hum. Mol. Genet."},{"key":"B169","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/s0006-291x(03)01322-6","article-title":"Expression of Class III facilitative glucose transporter genes (GLUT-10 and GLUT-12) in mouse and human adipose tissues","volume":"308","author":"Wood","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B170","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1038\/s41433-019-0603-z","article-title":"A review of systemic medications that may modulate the risk of glaucoma","volume":"34","author":"Wu","year":"2020","journal-title":"Eye Lond. Engl."},{"key":"B171","doi-asserted-by":"publisher","first-page":"100107","DOI":"10.1016\/j.xops.2021.100107","article-title":"Trabecular meshwork mitochondrial function and oxidative stress: Clues to racial disparities of glaucoma","volume":"2","author":"Wu","year":"2022","journal-title":"Ophthalmol. Sci."},{"key":"B172","doi-asserted-by":"publisher","first-page":"e0162048","DOI":"10.1371\/journal.pone.0162048","article-title":"OCT study of mechanical properties associated with trabecular meshwork and collector channel motion in human eyes","volume":"11","author":"Xin","year":"2016","journal-title":"PLoS One"},{"key":"B173","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.exer.2016.06.007","article-title":"Aqueous outflow regulation: Optical coherence tomography implicates pressure-dependent tissue motion","volume":"158","author":"Xin","year":"2017","journal-title":"Exp. Eye Res."},{"key":"B174","doi-asserted-by":"publisher","first-page":"1648","DOI":"10.1681\/asn.2014070678","article-title":"Myocardin-related transcription factor A epigenetically regulates renal fibrosis in diabetic nephropathy","volume":"26","author":"Xu","year":"2015","journal-title":"J. Am. Soc. Nephrol."},{"key":"B175","doi-asserted-by":"publisher","first-page":"4799","DOI":"10.1096\/fj.13-235127","article-title":"VBP1 facilitates proteasome and autophagy-mediated degradation of MutS homologue hMSH4","volume":"27","author":"Xu","year":"2013","journal-title":"Faseb J."},{"key":"B176","doi-asserted-by":"publisher","first-page":"E4596","DOI":"10.1073\/pnas.1412271111","article-title":"Structural basis of thymosin-\u03b24\/profilin exchange leading to actin filament polymerization","volume":"111","author":"Xue","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B177","doi-asserted-by":"publisher","first-page":"1863","DOI":"10.1101\/gr.1272403","article-title":"Decay rates of human mRNAs: Correlation with functional characteristics and sequence attributes","volume":"13","author":"Yang","year":"2003","journal-title":"Genome Res."},{"key":"B178","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1167\/iovs.61.5.2","article-title":"Expression of mRNAs, miRNAs, and lncRNAs in human trabecular meshwork cells upon mechanical stretch","volume":"61","author":"Youngblood","year":"2020","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"B179","doi-asserted-by":"publisher","first-page":"H1472","DOI":"10.1152\/ajpheart.00964.2009","article-title":"Knockout of Na+\/Ca2+ exchanger in smooth muscle attenuates vasoconstriction and L-type Ca2+ channel current and lowers blood pressure","volume":"298","author":"Zhang","year":"2010","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"B180","doi-asserted-by":"publisher","first-page":"50566","DOI":"10.1074\/jbc.m406743200","article-title":"Negative regulation of p53 functions by Daxx and the involvement of MDM2","volume":"279","author":"Zhao","year":"2004","journal-title":"J. Biol. Chem."},{"key":"B181","doi-asserted-by":"publisher","first-page":"1643","DOI":"10.1080\/15384101.2016.1170261","article-title":"Pro-fibrotic pathway activation in trabecular meshwork and lamina cribrosa is the main driving force of glaucoma","volume":"15","author":"Zhavoronkov","year":"2016","journal-title":"Cell. Cycle"},{"key":"B182","doi-asserted-by":"publisher","first-page":"e102972e102972","DOI":"10.1371\/journal.pone.0102972","article-title":"Diabetes mellitus as a risk factor for open-angle glaucoma: A systematic review and meta-analysis","volume":"9","author":"Zhou","year":"2014","journal-title":"PloS one"},{"key":"B183","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/2045-3701-1-44","article-title":"Unraveling the biological functions of Smad7 with mouse models","volume":"1","author":"Zhu","year":"2011","journal-title":"Cell. Biosci."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2022.874828\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T06:55:55Z","timestamp":1663052155000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2022.874828\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,13]]},"references-count":183,"alternative-id":["10.3389\/fcell.2022.874828"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2022.874828","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,13]]},"article-number":"874828"}}