{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T19:54:26Z","timestamp":1792439666020,"version":"4.5.0"},"reference-count":96,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>\n                    Assisted Reproductive Technologies (ART) employ gamete\/embryo handling and culture\n                    <jats:italic>in vitro<\/jats:italic>\n                    to produce offspring. ART pregnancies have an increased risk of low birth weight, abnormal placentation, pregnancy complications, and imprinting disorders. Embryo culture induces low birth weight, abnormal placental morphology, and lower levels of DNA methylation in placentas in a mouse model of ART. Whether preimplantation embryos at specific stages of development are more susceptible to these perturbations remains unresolved. Accordingly, we performed embryo culture for several discrete periods of preimplantation development and following embryo transfer, assessed fetal and placental outcomes at term. We observed a reduction in fetal:placental ratio associated with two distinct windows of preimplantation embryo development, one prior to the morula stage and the other from the morula to blastocyst stage, whereas placental morphological abnormalities and reduced imprinting control region methylation were only associated with culture prior to the morula stage. Extended culture to the blastocyst stage also induces additional placental DNA methylation changes compared to embryos transferred at the morula stage, and female concepti exhibited a higher loss of DNA methylation than males. By identifying specific developmental windows of susceptibility, this study provides a framework to optimize further culture conditions to minimize risks associated with ART pregnancies.\n                  <\/jats:p>","DOI":"10.3389\/fcell.2022.884088","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T02:11:56Z","timestamp":1650852716000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["Placental Abnormalities are Associated With Specific Windows of Embryo Culture in a Mouse Model"],"prefix":"10.3389","volume":"10","author":[{"given":"Lisa A.","family":"Vrooman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric A.","family":"Rhon-Calderon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kashviya V.","family":"Suri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asha K.","family":"Dahiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yemin","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Schultz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa S.","family":"Bartolomei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1006\/DBIO.1996.8466","article-title":"Regulation of Transcriptional Activity during the First and Second Cell Cycles in the Preimplantation Mouse Embryo","volume":"181","author":"Aoki","year":"1997","journal-title":"Developmental Biol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1186\/s13059-014-0545-5","article-title":"Ontogeny of CpG Island Methylation and Specificity of DNMT3 Methyltransferases during Embryonic Development in the Mouse","volume":"15","author":"Auclair","year":"2014","journal-title":"Genome Biol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1016\/0093-691X(91)90306-X","article-title":"Sex and Development in Bovine In-Vitro Fertilized Embryos","volume":"35","author":"Avery","year":"1991","journal-title":"Theriogenology"},{"key":"B4","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1016\/B978-0-12-394445-0.00050-3","article-title":"50 \u2013 Nonradioactive In Situ Hybridization: Optimization for Tissue Sections from Pregnant Uteri and Placenta during the First Half of Pregnancy","volume":"2014","author":"Bany","year":"2014","journal-title":"Guide Invest. Mouse Pregnancy"},{"key":"B5","doi-asserted-by":"publisher","first-page":"a018382","DOI":"10.1101\/cshperspect.a018382","article-title":"Genomic Imprinting in Mammals","volume":"6","author":"Barlow","year":"2014","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"B6","first-page":"92","article-title":"Mouse Colony Setup","volume-title":"Manipulating The Mouse Embryo: A Laboratory Manual","author":"Behringer","year":"2014"},{"key":"B7","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1093\/HUMREP\/11.3.621","article-title":"Sex-related Differences in the Developmental Rate of In-Vitro Matured\/in-Vitro Fertilized Ovine Embryos","volume":"11","author":"Bernardi","year":"1996","journal-title":"Hum. Reprod."},{"key":"B96","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/15592294.2021.1985300","article-title":"Associations Between Infant Sex and DNA Methylation Across Umbilical Cord Blood, Artery, and Placenta Aamples","volume":"22","author":"Bozack","year":"2021","journal-title":"Epigenetics"},{"key":"B8","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1007\/S43032-020-00355-8","article-title":"Examining Sex Differences in the Human Placental Transcriptome during the First Fetal Androgen Peak","volume":"28","author":"Braun","year":"2021","journal-title":"Reprod. Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1016\/J.FERTNSTERT.2014.10.050","article-title":"Is There a Relationship between Time-Lapse Parameters and Embryo Sex?","volume":"103","author":"Bronet","year":"2015","journal-title":"Fertil. Sterility"},{"key":"B10","doi-asserted-by":"publisher","first-page":"2381","DOI":"10.1093\/BIOINFORMATICS\/BTX183","article-title":"Annotatr: Genomic Regions in Context","volume":"33","author":"Cavalcante","year":"2017","journal-title":"Bioinformatics"},{"key":"B11","doi-asserted-by":"publisher","first-page":"2381","DOI":"10.1016\/j.fertnstert.2008.03.066","article-title":"Impact of Blastocyst Transfer on Offspring Sex Ratio and the Monozygotic Twinning Rate: a Systematic Review and Meta-Analysis","volume":"91","author":"Chang","year":"2009","journal-title":"Fertil. Sterility"},{"key":"B12","doi-asserted-by":"publisher","first-page":"10596","DOI":"10.1038\/srep10596","article-title":"Assisted Reproduction Causes Placental Maldevelopment and Dysfunction Linked to Reduced Fetal Weight in Mice","volume":"5","author":"Chen","year":"2015","journal-title":"Sci. Rep."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1007\/S00404-018-4905-3","article-title":"Effects of Superovulation, In Vitro Fertilization, and Oocyte In Vitro Maturation on Imprinted Gene Grb10 in Mouse Blastocysts","volume":"298","author":"Chen","year":"2018","journal-title":"Arch. Gynecol. Obstet."},{"key":"B14","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1172\/JCI41229","article-title":"Making the Blastocyst: Lessons from the Mouse","volume":"120","author":"Cockburn","year":"2010","journal-title":"J. Clin. Invest."},{"key":"B15","doi-asserted-by":"publisher","first-page":"35543","DOI":"10.1074\/JBC.M111.227637","article-title":"Placenta-specific Expression of the Interleukin-2 (IL-2) Receptor \u03b2 Subunit from an Endogenous Retroviral Promoter","volume":"286","author":"Cohen","year":"2011","journal-title":"J. Biol. Chem."},{"key":"B16","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1007\/S10815-018-1173-X","article-title":"Comprehensive Meta-Analysis Reveals Association between Multiple Imprinting Disorders and conception by Assisted Reproductive Technology","volume":"35","author":"Cortessis","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1093\/humrep\/14.4.1107","article-title":"Do placentae of Term Singleton Pregnancies Obtained by Assisted Reproductive Technologies Differ from Those of Spontaneously Conceived Pregnancies?","volume":"14","author":"Daniel","year":"1999","journal-title":"Hum. Reprod."},{"key":"B18","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1093\/humupd\/dmu001","article-title":"Neonatal Outcomes Among Singleton Births after Blastocyst versus Cleavage Stage Embryo Transfer: A Systematic Review and Meta-Analysis","volume":"20","author":"Dar","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"B19","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1093\/humrep\/des448","article-title":"Increased Risk of Preterm Birth in Singleton Pregnancies after Blastocyst versus Day 3 Embryo Transfer: Canadian ART Register (CARTR) Analysis","volume":"28","author":"Dar","year":"2013","journal-title":"Hum. Reprod."},{"key":"B20","doi-asserted-by":"publisher","first-page":"2442","DOI":"10.1093\/humrep\/dew219","article-title":"Cumulative Live Birth Rates after Fresh and Vitrified Cleavage-Stage versus Blastocyst-Stage Embryo Transfer in the First Treatment Cycle","volume":"31","author":"De Vos","year":"2016","journal-title":"Hum. Reprod."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1095\/biolreprod.113.114785","article-title":"In Vitro Culture Increases the Frequency of Stochastic Epigenetic Errors at Imprinted Genes in Placental Tissues from Mouse Concepti Produced through Assisted Reproductive Technologies 1","volume":"90","author":"de Waal","year":"2014","journal-title":"Biol. Reprod."},{"key":"B22","doi-asserted-by":"publisher","first-page":"ddv400","DOI":"10.1093\/hmg\/ddv400","article-title":"The Cumulative Effect of Assisted Reproduction Procedures on Placental Development and Epigenetic Perturbations in a Mouse Model","volume":"24","author":"de Waal","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"B23","doi-asserted-by":"publisher","first-page":"4163","DOI":"10.1073\/pnas.1201990109","article-title":"Primary Epimutations Introduced during Intracytoplasmic Sperm Injection (ICSI) Are Corrected by Germline-specific Epigenetic Reprogramming","volume":"109","author":"de Waal","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B24","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1086\/346031","article-title":"Association of In Vitro Fertilization with Beckwith-Wiedemann Syndrome and Epigenetic Alterations of LIT1 and H19","volume":"72","author":"DeBaun","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1702","DOI":"10.1093\/MOLBEV\/MSX112","article-title":"DNA Methylation Divergence and Tissue Specialization in the Developing Mouse Placenta","volume":"34","author":"Decato","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"2039","DOI":"10.1093\/humrep\/deq165","article-title":"Effect of the Method of conception and Embryo Transfer Procedure on Mid-gestation Placenta and Fetal Development in an IVF Mouse Model","volume":"25","author":"Delle Piane","year":"2010","journal-title":"Hum. Reprod."},{"key":"B27","doi-asserted-by":"publisher","first-page":"e16252","DOI":"10.1371\/JOURNAL.PONE.0016252","article-title":"Methylation at Global LINE-1 Repeats in Human Blood Are Affected by Gender but Not by Age or Natural Hormone Cycles","volume":"6","author":"El-Maarri","year":"2011","journal-title":"PLoS One"},{"key":"B28","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1016\/j.rbmo.2011.08.011","article-title":"Blastocyst-stage versus Cleavage-Stage Embryo Transfer in Women with High Oestradiol Concentrations: Randomized Controlled Trial","volume":"23","author":"Elgindy","year":"2011","journal-title":"Reprod. BioMedicine Online"},{"key":"B29","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1093\/BFGP\/ELS013","article-title":"Transposable Element Recruitments in the Mammalian Placenta: Impacts and Mechanisms","volume":"11","author":"Emera","year":"2012","journal-title":"Brief. Funct. Genomics"},{"key":"B30","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1007\/s10815-014-0387-9","article-title":"Ongoing and Cumulative Pregnancy Rate after Cleavage-Stage versus Blastocyst-Stage Embryo Transfer Using Vitrification for Cryopreservation: Impact of Age on the Results","volume":"32","author":"Fern\u00e1ndez-Shaw","year":"2015","journal-title":"J. Assist. Reprod. Genet."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1653","DOI":"10.1093\/hmg\/ddn055","article-title":"Superovulation Alters the Expression of Imprinted Genes in the Midgestation Mouse Placenta","volume":"17","author":"Fortier","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"B32","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.mce.2014.03.003","article-title":"Modulation of Imprinted Gene Expression Following Superovulation","volume":"388","author":"Fortier","year":"2014","journal-title":"Mol. Cell Endocrinol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/S0015-0282(02)04558-2","article-title":"Blastocyst Transfer Decreases Multiple Pregnancy Rates in In Vitro Fertilization Cycles: a Randomized Controlled Trial","volume":"79","author":"Frattarelli","year":"2003","journal-title":"Fertil. Sterility"},{"key":"B34","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/S13148-017-0318-6","article-title":"Global DNA Methylation Levels Are Altered by Modifiable Clinical Manipulations in Assisted Reproductive Technologies","volume":"9","author":"Ghosh","year":"2017","journal-title":"Clin. Epigenet"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1016\/0002-9610(92)90118-B10.1086\/374824","article-title":"In Vitro Fertilization May Increase the Risk of Beckwith-Wiedemann Syndrome Related to the Abnormal Imprinting of the KCNQ1OT Gene","volume":"72","author":"Gicquel","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"B36","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1016\/j.ajog.2015.12.040","article-title":"Neonatal and Maternal Outcome after Blastocyst Transfer: a Population-Based Registry Study","volume":"214","author":"Ginstr\u00f6m Ernstad","year":"2016","journal-title":"Am. J. Obstet. Gynecol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"CD002118","DOI":"10.1002\/14651858.CD002118.pub5","article-title":"Cleavage Stage versus Blastocyst Stage Embryo Transfer in Assisted Reproductive Technology","volume":"2016","author":"Glujovsky","year":"2016","journal-title":"Cochrane Database Syst. Rev."},{"key":"B38","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1080\/15592294.2018.1429857","article-title":"Genome-wide Oxidative Bisulfite Sequencing Identifies Sex-specific Methylation Differences in the Human Placenta","volume":"13","author":"Gong","year":"2018","journal-title":"Epigenetics"},{"key":"B39","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/S13293-018-0165-Y","article-title":"Sex Differences in the Late First Trimester Human Placenta Transcriptome","volume":"9","author":"Gonzalez","year":"2018","journal-title":"Biol. Sex. Differ."},{"key":"B40","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1093\/humrep\/der428","article-title":"Placental Weight in Singleton Pregnancies with and without Assisted Reproductive Technology: a Population Study of 536 567 Pregnancies","volume":"27","author":"Haavaldsen","year":"2012","journal-title":"Hum. Reprod."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S13148-019-0623-3","article-title":"Association of Four Imprinting Disorders and ART","volume":"11","author":"Hattori","year":"","journal-title":"Clin. Epigenet"},{"key":"B42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12958-019-0471-1","article-title":"The Risk of Secondary Sex Ratio Imbalance and Increased Monozygotic Twinning after Blastocyst Transfer: Data from the Japan Environment and Children's Study","volume":"17","author":"Hattori","year":"","journal-title":"Reprod. Biol. Endocrinol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphys.2016.00028","article-title":"Placental Adaptation: What Can We Learn from Birthweight:placental Weight Ratio?","volume":"7","author":"Hayward","year":"2016","journal-title":"Front. Physiol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"1178","DOI":"10.1093\/HUMREP\/DEAA039","article-title":"Imprinting Disorders in Children Born after ART: a Nordic Study from the CoNARTaS Group","volume":"35","author":"Henningsen","year":"2020","journal-title":"Hum. Reprod."},{"key":"B45","doi-asserted-by":"publisher","first-page":"2541","DOI":"10.1093\/HUMREP\/DES197","article-title":"Characterization of DNA Methylation Errors in Patients with Imprinting Disorders Conceived by Assisted Reproduction Technologies","volume":"27","author":"Hiura","year":"2012","journal-title":"Hum. Reprod."},{"key":"B46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S13148-020-00866-W","article-title":"Single-cell DNA Methylation Sequencing Reveals Epigenetic Alterations in Mouse Oocytes Superovulated with Different Dosages of Gonadotropins","volume":"12","author":"Huo","year":"2020","journal-title":"Clin. Epigenet"},{"key":"B47","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1093\/IJE\/DYR238","article-title":"Bump Hunting to Identify Differentially Methylated Regions in Epigenetic Epidemiology Studies","volume":"41","author":"Jaffe","year":"2012","journal-title":"Int. J. Epidemiol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"1966","DOI":"10.1093\/BIOINFORMATICS\/BTY890","article-title":"TissueEnrich: Tissue-specific Gene Enrichment Analysis","volume":"35","author":"Jain","year":"2019","journal-title":"Bioinformatics"},{"key":"B49","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1007\/s10815-018-1228-z","article-title":"Overrepresentation of Pregnancies Conceived by Artificial Reproductive Technology in Prenatally Identified Fetuses with Beckwith-Wiedemann Syndrome","volume":"35","author":"Johnson","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"B50","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1038\/NRG3230","article-title":"Functions of DNA Methylation: Islands, Start Sites, Gene Bodies and beyond","volume":"13","author":"Jones","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"B51","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1097\/AOG.0b013e31825b88fc","article-title":"Extended Embryo Culture and an Increased Risk of Preterm Delivery","volume":"120","author":"Kalra","year":"2012","journal-title":"Obstet. Gynecol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"3296","DOI":"10.1016\/J.CELREP.2020.02.048","article-title":"Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation","volume":"30","author":"Lu","year":"2020","journal-title":"Cel Rep."},{"key":"B53","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1136\/jmg.2004.026930","article-title":"Increased Prevalence of Imprinting Defects in Patients with Angelman Syndrome Born to Subfertile Couples","volume":"42","author":"Ludwig","year":"2005","journal-title":"J. Med. Genet."},{"key":"B54","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1016\/j.ajog.2019.10.003","article-title":"In Vitro fertilization and Risk for Hypertensive Disorders of Pregnancy: Associations with Treatment Parameters","volume":"222","author":"Luke","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/s10815-016-0850-x","article-title":"Increased Risk of Large-For-Gestational Age Birthweight in Singleton Siblings Conceived with In Vitro Fertilization in Frozen versus Fresh Cycles","volume":"34","author":"Luke","year":"2017","journal-title":"J. Assist. Reprod. Genet."},{"key":"B56","doi-asserted-by":"publisher","first-page":"e95840","DOI":"10.1371\/JOURNAL.PONE.0095840","article-title":"Retrotransposon Hypomethylation in Melanoma and Expression of a Placenta-specific Gene","volume":"9","author":"Macaulay","year":"2014","journal-title":"PLoS One"},{"key":"B57","doi-asserted-by":"publisher","first-page":"3727","DOI":"10.1242\/dev.01241","article-title":"Selective Loss of Imprinting in the Placenta Following Preimplantation Development in Culture","volume":"131","author":"Mann","year":"2004","journal-title":"Development"},{"key":"B58","doi-asserted-by":"publisher","first-page":"e80398","DOI":"10.1371\/journal.pone.0080398","article-title":"Perinatal Outcomes by Mode of Assisted conception and Sub-fertility in an Australian Data Linkage Cohort","volume":"9","author":"Marino","year":"2014","journal-title":"PLoS One"},{"key":"B59","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1095\/biolreprod.110.085480","article-title":"Side-by-Side Comparison of Five Commercial Media Systems in a Mouse Model: Suboptimal In Vitro Culture Interferes with Imprint Maintenance1","volume":"83","author":"Market-Velker","year":"2010","journal-title":"Biol. Reprod."},{"key":"B60","doi-asserted-by":"publisher","first-page":"267","DOI":"10.2217\/EPI-2016-0132","article-title":"Sexual Epigenetic Dimorphism in the Human Placenta: Implications for Susceptibility during the Prenatal Period","volume":"9","author":"Martin","year":"2017","journal-title":"Epigenomics"},{"key":"B61","doi-asserted-by":"publisher","first-page":"279","DOI":"10.2217\/EPI-2016-0103","article-title":"Accelerated Placental Aging in Early Onset Preeclampsia Pregnancies Identified by DNA Methylation","volume":"9","author":"Mayne","year":"2017","journal-title":"Epigenomics"},{"key":"B62","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1101\/GAD.234294.113","article-title":"DNA Methylation Dynamics during Epigenetic Reprogramming in the Germline and Preimplantation Embryos","volume":"28","author":"Messerschmidt","year":"2014","journal-title":"Genes Dev."},{"key":"B63","doi-asserted-by":"publisher","first-page":"170210","DOI":"10.1098\/RSOB.170210","article-title":"The First Cell-Fate Decision of Mouse Preimplantation Embryo Development: Integrating Cell Position and Polarity","volume":"7","author":"Mihajlovi\u0107","year":"2017","journal-title":"Open Biol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"e20164311","DOI":"10.1542\/PEDS.2016-4311","article-title":"Assisted Reproductive Techniques and Risk of Beckwith-Wiedemann Syndrome","volume":"140","author":"Mussa","year":"2017","journal-title":"Pediatrics"},{"key":"B65","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/S12978-020-0870-Y","article-title":"Non-inferiority of Cleavage-Stage versus Blastocyst-Stage Embryo Transfer in Poor Prognosis IVF Patients (PRECiSE Trial): Study Protocol for a Randomized Controlled Trial","volume":"17","author":"Neuhausser","year":"2020","journal-title":"Reprod. Health"},{"key":"B66","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/J.PLACENTA.2011.12.013","article-title":"DNA Methylation of Stress-Related Genes and LINE-1 Repetitive Elements across the Healthy Human Placenta","volume":"33","author":"Non","year":"2012","journal-title":"Placenta"},{"key":"B67","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1093\/humupd\/dms018","article-title":"Obstetric and Perinatal Outcomes in Singleton Pregnancies Resulting from IVF\/ICSI: a Systematic Review and Meta-Analysis","volume":"18","author":"Pandey","year":"2012","journal-title":"Hum. Reprod. Update"},{"key":"B68","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1097\/01.ogx.0000228704.71058.7b","article-title":"In Vitro fertilization with Single Blastocyst-Stage versus Single Cleavage-Stage Embryos","volume":"61","author":"Papanikolaou","year":"2006","journal-title":"Obstetrical Gynecol. Surv."},{"key":"B69","doi-asserted-by":"publisher","first-page":"3198","DOI":"10.1093\/humrep\/dei217","article-title":"Live Birth Rate Is Significantly Higher after Blastocyst Transfer Than after Cleavage-Stage Embryo Transfer when at Least Four Embryos Are Available on Day 3 of Embryo Culture. A Randomized Prospective Study","volume":"20","author":"Papanikolaou","year":"2005","journal-title":"Hum. Reprod."},{"key":"B70","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1093\/BIOINFORMATICS\/BTQ033","article-title":"BEDTools: a Flexible Suite of Utilities for Comparing Genomic Features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"B71","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/hmg\/ddm280","article-title":"Manipulations of Mouse Embryos Prior to Implantation Result in Aberrant Expression of Imprinted Genes on Day 9.5 of Development","volume":"17","author":"Rivera","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"B72","doi-asserted-by":"publisher","first-page":"9324","DOI":"10.1073\/PNAS.161271798","article-title":"Placental Defects and Embryonic Lethality in Mice Lacking Suppressor of Cytokine Signaling 3","volume":"98","author":"Roberts","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B73","doi-asserted-by":"publisher","first-page":"2165","DOI":"10.1007\/S10815-021-02235-9","article-title":"Morphology-based Selection from Available Euploid Blastocysts Induces Male-Skewed Sex Proportion in the Offspring","volume":"38","author":"Roos Kulmann","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"B74","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1016\/J.FERTNSTERT.2021.12.017","article-title":"Placental Pathology of Term Singleton Live Births Conceived with Fresh Embryo Transfer Compared with Those Conceived without Assisted Reproductive Technology","volume":"117","author":"Sacha","year":"2022","journal-title":"Fertil. Sterility"},{"key":"B75","doi-asserted-by":"publisher","first-page":"dev160622","DOI":"10.1242\/dev.160622","article-title":"Imprinted Gene Dysregulation in a Tet1 Null Mouse Model Is Stochastic and Variable in the Germline and Offspring","volume":"145","author":"SanMiguel","year":"2018","journal-title":"Development"},{"key":"B76","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1093\/HUMREP\/DEL316","article-title":"Aberrant DNA Methylation of Imprinted Loci in Superovulated Oocytes","volume":"22","author":"Sato","year":"2007","journal-title":"Hum. Reprod."},{"key":"B77","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1007\/s10995-007-0202-7","article-title":"A Population-Based Study of Maternal and Perinatal Outcomes Associated with Assisted Reproductive Technology in Massachusetts","volume":"11","author":"Schieve","year":"2007","journal-title":"Matern. Child. Health J."},{"key":"B78","volume-title":"The Guide to Investigation of Mouse Pregnancy","author":"Simmons","year":"2014"},{"key":"B79","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1016\/J.PLACENTA.2010.07.011","article-title":"Wnt Signalling in Implantation, Decidualisation and Placental Differentiation - Review","volume":"31","author":"Sonderegger","year":"2010","journal-title":"Placenta"},{"key":"B80","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1093\/HUMREP\/DEAA032","article-title":"Obstetric and Perinatal Risks in 4601 Singletons and 884 Twins Conceived after Fresh Blastocyst Transfers: A Nordic Study from the CoNARTaS Group","volume":"35","author":"Spangmose","year":"2020","journal-title":"Hum. Reprod."},{"key":"B81","doi-asserted-by":"publisher","first-page":"1585","DOI":"10.1007\/s10815-018-1238-x","article-title":"Birth Outcomes of Singleton Vaginal Deliveries to ART-Treated, Subfertile, and fertile Primiparous Women","volume":"35","author":"Stern","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"B82","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1095\/biolreprod.114.124248","article-title":"Dynamic Proteomic Profiles of In Vivo- and In Vitro-Produced Mouse Postimplantation Extraembryonic Tissues and Placentas1","volume":"91","author":"Sui","year":"2014","journal-title":"Biol. Reprod."},{"key":"B83","doi-asserted-by":"publisher","first-page":"e4831","DOI":"10.1210\/CLINEM\/DGAA503","article-title":"Sexually Dimorphic Crosstalk at the Maternal-Fetal Interface","volume":"105","author":"Sun","year":"2020","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"B84","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1016\/J.EJMG.2018.05.017","article-title":"Assisted Reproductive Technologies and Imprinting Disorders: Results of a Study from a French Congenital Malformations Registry","volume":"61","author":"Uk","year":"2018","journal-title":"Eur. J. Med. Genet."},{"key":"B85","doi-asserted-by":"publisher","first-page":"858","DOI":"10.3389\/FGENE.2019.00858","article-title":"pathfindR: An R Package for Comprehensive Identification of Enriched Pathways in Omics Data through Active Subnetworks","volume":"10","author":"Ulgen","year":"2019","journal-title":"Front. Genet."},{"key":"B86","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1002\/MRD.1080350204","article-title":"PCR Sexing and Developmental Rate Differences in Preimplantation Mouse Embryos Fertilized and Cultured In Vitro","volume":"35","author":"Valdivia","year":"1993","journal-title":"Mol. Reprod. Dev."},{"key":"B87","doi-asserted-by":"publisher","first-page":"dvx015","DOI":"10.1093\/EEP\/DVX015","article-title":"Maintenance of Mest Imprinted Methylation in Blastocyst-Stage Mouse Embryos Is Less Stable Than Other Imprinted Loci Following Superovulation or Embryo Culture","volume":"3","author":"Velker","year":"2017","journal-title":"Environ. Epigenetics"},{"key":"B88","doi-asserted-by":"publisher","first-page":"e1008236","DOI":"10.1371\/JOURNAL.PGEN.1008236","article-title":"Human Placental Methylome in the Interplay of Adverse Placental Health, Environmental Exposure, and Pregnancy Outcome","volume":"15","author":"Vlahos","year":"2019","journal-title":"Plos Genet."},{"key":"B89","doi-asserted-by":"publisher","first-page":"dev186551","DOI":"10.1242\/dev.186551","article-title":"Assisted Reproductive Technologies Induce Temporally Specific Placental Defects and the Preeclampsia Risk Marker sFLT1 in Mouse","volume":"147","author":"Vrooman","year":"2020","journal-title":"Development"},{"key":"B90","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12958-017-0255-4","article-title":"Comparative Neonatal Outcomes in Singleton Births from Blastocyst Transfers or Cleavage-Stage Embryo Transfers: A Systematic Review and Meta-Analysis","volume":"15","author":"Wang","year":"2017","journal-title":"Reprod. Biol. Endocrinol."},{"key":"B91","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1289\/EHP.1103927","article-title":"In Utero exposures, Infant Growth, and DNA Methylation of Repetitive Elements and Developmentally Related Genes in Human Placenta","volume":"120","author":"Wilhelm-Benartzi","year":"2012","journal-title":"Environ. Health Perspect."},{"key":"B92","doi-asserted-by":"publisher","first-page":"2102","DOI":"10.1016\/j.fertnstert.2010.01.014","article-title":"In Vitro fertilization and Preterm Delivery, Low Birth Weight, and Admission to the Neonatal Intensive Care Unit: a Prospective Follow-Up Study","volume":"94","author":"Wisborg","year":"2010","journal-title":"Fertil. Sterility"},{"key":"B93","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1080\/15592294.2019.1615353","article-title":"Superovulation Alters Global DNA Methylation in Early Mouse Embryo Development","volume":"14","author":"Yu","year":"2019","journal-title":"Epigenetics"},{"key":"B94","doi-asserted-by":"publisher","first-page":"2382","DOI":"10.1093\/BIOINFORMATICS\/BTV145","article-title":"ChIPseeker: an R\/Bioconductor Package for ChIP Peak Annotation, Comparison and Visualization","volume":"31","author":"Yu","year":"2015","journal-title":"Bioinformatics"},{"key":"B95","doi-asserted-by":"crossref","DOI":"10.1016\/j.xgen.2022.100144","article-title":"DNA Methylation Dynamics and Dysregulation Delineated by High-Throughput Profiling in the Mouse","author":"Zhou","year":"2022","journal-title":"Cell Genomics"}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2022.884088\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T03:10:15Z","timestamp":1675393815000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2022.884088\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,25]]},"references-count":96,"alternative-id":["10.3389\/fcell.2022.884088"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2022.884088","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,25]]},"article-number":"884088"}}