{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:35:22Z","timestamp":1791959722441,"version":"4.3.4"},"reference-count":175,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>The sensory epithelium of the mammalian cochlea, the organ of Corti, is comprised of at least seven unique cell types including two functionally distinct types of mechanosensory hair cells. All of the cell types within the organ of Corti are believed to develop from a population of precursor cells referred to as prosensory cells. Results from previous studies have begun to identify the developmental processes, lineage restrictions and signaling networks that mediate the specification of many of these cell types, however, the small size of the organ and the limited number of each cell type has hampered progress. Recent technical advances, in particular relating to the ability to capture and characterize gene expression at the single cell level, have opened new avenues for understanding cellular specification in the organ of Corti. This review will cover our current understanding of cellular specification in the cochlea, discuss the most commonly used methods for single cell RNA sequencing and describe how results from a recent study using single cell sequencing provided new insights regarding cellular specification.<\/jats:p>","DOI":"10.3389\/fcell.2022.884240","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T08:52:14Z","timestamp":1655974334000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Cochlear Development; New Tools and Approaches"],"prefix":"10.3389","volume":"10","author":[{"given":"Matthew W.","family":"Kelley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1242\/dev.071670","article-title":"EYA1 and SIX1 Drive the Neuronal Developmental Program in Cooperation with the SWI\/SNF Chromatin-Remodeling Complex and SOX2 in the Mammalian Inner Ear","volume":"139","author":"Ahmed","year":"2012","journal-title":"Development"},{"key":"B2","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.neuron.2020.12.010","article-title":"Single-Cell Sequencing of Brain Cell Transcriptomes and Epigenomes","volume":"109","author":"Armand","year":"2021","journal-title":"Neuron"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.3390\/biomedicines9111513","article-title":"Single-Cell Analysis Using Machine Learning Techniques and its Application to Medical Research","volume":"9","author":"Asada","year":"2021","journal-title":"Biomedicines"},{"key":"B4","doi-asserted-by":"publisher","first-page":"a033522","DOI":"10.1101\/cshperspect.a033522","article-title":"Outer Hair Cells and Electromotility","volume":"9","author":"Ashmore","year":"2019","journal-title":"Cold Spring Harb. 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