{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:42:12Z","timestamp":1791549732054,"version":"4.3.4"},"reference-count":73,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","award":["22-10660S"],"award-info":[{"award-number":["22-10660S"]}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["LM2015040 LM2018129"],"award-info":[{"award-number":["LM2015040 LM2018129"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["1034120 340321"],"award-info":[{"award-number":["1034120 340321"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>A vertebrate skull is composed of many skeletal elements which display enormous diversity of shapes. Cranial bone formation embodies a multitude of processes, i.e., epithelial-mesenchymal induction, mesenchymal condensation, and endochondral or intramembranous ossification. Molecular pathways determining complex architecture and growth of the cranial skeleton during embryogenesis are poorly understood. Here, we present a model of the hyoid apparatus development in Wnt1-Cre2-induced <jats:italic>Meis2<\/jats:italic> conditional knock-out (cKO) mice. <jats:italic>Meis2<\/jats:italic> cKO embryos develop an aberrant hyoid apparatus\u2014a complete skeletal chain from the base of the neurocranium to lesser horns of the hyoid, resembling extreme human pathologies of the hyoid-larynx region. We examined key stages of hyoid skeletogenesis to obtain a complex image of the hyoid apparatus formation. Lack of <jats:italic>Meis2<\/jats:italic> resulted in ectopic loci of mesenchymal condensations, ectopic cartilage and bone formation, disinhibition of skeletogenesis, and elevated proliferation of cartilage precursors. We presume that all these mechanisms contribute to formation of the aberrant skeletal chain in the hyoid region. Moreover, <jats:italic>Meis2<\/jats:italic> cKO embryos exhibit severely reduced expression of PBX1 and HAND2 in the hyoid region. Altogether, MEIS2 in conjunction with PBX1 and HAND2 affects mesenchymal condensation, specification and proliferation of cartilage precursors to ensure development of the anatomically correct hyoid apparatus.<\/jats:p>","DOI":"10.3389\/fcell.2022.951063","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T05:41:27Z","timestamp":1664343687000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Meis2 controls skeletal formation in the hyoid region"],"prefix":"10.3389","volume":"10","author":[{"given":"Jaroslav","family":"Fabik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktorie","family":"Psutkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Machon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1016\/j.bone.2009.11.022","article-title":"Hand2 regulates chondrogenesis in vitro and in vivo","volume":"46","author":"Abe","year":"2010","journal-title":"Bone"},{"key":"B2","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.devcel.2014.12.024","article-title":"Hoxa2 selectively enhances meis binding to change a branchial arch ground state","volume":"32","author":"Amin","year":"2015","journal-title":"Dev. Cell"},{"key":"B3","doi-asserted-by":"publisher","first-page":"2599","DOI":"10.1002\/dvdy.22092","article-title":"Prx1 and Prx2 cooperatively regulate the morphogenesis of the medial region of the mandibular process","volume":"238","author":"Balic","year":"2009","journal-title":"Dev. Dyn."},{"key":"B4","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.ydbio.2007.07.036","article-title":"Hand transcription factors cooperatively regulate development of the distal midline mesenchyme","volume":"310","author":"Barbosa","year":"2007","journal-title":"Dev. Biol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1242\/dev.056929","article-title":"Downregulation of Dlx5 and Dlx6 expression by Hand2 is essential for initiation of tongue morphogenesis","volume":"138","author":"Barron","year":"2011","journal-title":"Development"},{"key":"B6","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1038\/ng0697-174","article-title":"SOX9 directly regulates the type-II collagen gene","volume":"16","author":"Bell","year":"1997","journal-title":"Nat. Genet."},{"key":"B7","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1002\/dvdy.24256","article-title":"Sonic hedgehog from pharyngeal arch 1 epithelium is necessary for early mandibular arch cell survival and later cartilage condensation differentiation","volume":"244","author":"Billmyre","year":"2015","journal-title":"Dev. Dyn."},{"key":"B8","doi-asserted-by":"publisher","first-page":"2263","DOI":"10.1242\/dev.02395","article-title":"Pbx1\/Pbx2 requirement for distal limb patterning is mediated by the hierarchical control of Hox gene spatial distribution and Shhexpression","volume":"133","author":"Capellini","year":"2006","journal-title":"Development"},{"key":"B9","article-title":"Eagle\u2019s syndrome with 3-D reconstructed CT: two cases report","author":"Chiang","year":"2004"},{"key":"B10","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.devcel.2009.08.007","article-title":"Meis cofactors control HDAC and CBP accessibility at Hox-regulated promoters during zebrafish embryogenesis","volume":"17","author":"Choe","year":"2009","journal-title":"Dev. Cell"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1829","DOI":"10.1002\/lary.26987","article-title":"The development of the human hyoid-larynx complex revisited","volume":"128","author":"de Bakker","year":"2018","journal-title":"Laryngoscope"},{"key":"B12","doi-asserted-by":"publisher","first-page":"15950","DOI":"10.1038\/s41598-019-52476-z","article-title":"Variants of the hyoid-larynx complex, with implications for forensic science and consequence for the diagnosis of Eagle\u2019s syndrome","volume":"9","author":"de Bakker","year":"2019","journal-title":"Sci. Rep."},{"key":"B13","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1002\/ar.22452","article-title":"Biometry and statistical analysis of the styloid process","volume":"295","author":"De Paz","year":"2012","journal-title":"Anat. Rec."},{"key":"B14","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1002\/jcb.10308","article-title":"Alterations in the spatiotemporal expression pattern and function of N-Cadherin inhibit cellular condensation and chondrogenesis of limb mesenchymal cells in vitro","volume":"87","author":"DeLise","year":"2002","journal-title":"J. Cell. Biochem."},{"key":"B15","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1471-2148-12-27","article-title":"Evolution of the osteoblast: Skeletogenesis in gar and zebrafish","volume":"12","author":"Eames","year":"2012","journal-title":"BMC Evol. Biol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"bio052043","DOI":"10.1242\/bio.052043","article-title":"Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway","volume":"9","author":"Fabik","year":"2020","journal-title":"Biol. Open"},{"key":"B17","doi-asserted-by":"publisher","first-page":"7529","DOI":"10.3390\/ijms22147529","article-title":"The mandibular and hyoid arches\u2014from molecular patterning to shaping bone and cartilage","volume":"22","author":"Fabik","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"2734","DOI":"10.2741\/3882","article-title":"Induction and patterning of intramembranous bone","volume":"16","author":"Franz-Odendaal","year":"2011","journal-title":"Front. Biosci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1002\/wdev.147","article-title":"Development and evolution of the pharyngeal apparatus","volume":"3","author":"Frisdal","year":"2014","journal-title":"Wiley Interdiscip. Rev. Dev. Biol."},{"key":"B73","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1242\/dev.029355","article-title":"Hand2 controls osteoblast differentiation in the branchial arch by inhibiting DNA binding of Runx2","volume":"136","author":"Funato","year":"2009","journal-title":"Develop."},{"key":"B20","doi-asserted-by":"publisher","first-page":"28405","DOI":"10.1038\/srep28405","article-title":"Specification of jaw identity by the Hand2 transcription factor","volume":"6","author":"Funato","year":"2016","journal-title":"Sci. Rep."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1317","DOI":"10.1016\/0092-8674(93)90619-2","article-title":"Hoxa-2 mutant mice exhibit homeotic transformation of skeletal elements derived from cranial neural crest","volume":"75","author":"Gendron-Maguire","year":"1993","journal-title":"Cell"},{"key":"B22","doi-asserted-by":"publisher","first-page":"E4","DOI":"10.3390\/jdb7010004","article-title":"The forgotten skeletogenic condensations: A comparison of early skeletal development amongst vertebrates","volume":"7","author":"Giffin","year":"2019","journal-title":"J. Dev. Biol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"3531","DOI":"10.1128\/MCB.00889-09","article-title":"Pbx1 represses osteoblastogenesis by blocking hoxa10-mediated recruitment of chromatin remodeling factors","volume":"30","author":"Gordon","year":"2010","journal-title":"Mol. Cell. Biol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1007\/BF00174948","article-title":"The membranous skeleton: the role of cell condensations in vertebrate skeletogenesis","volume":"186","author":"Hall","year":"1992","journal-title":"Anat. Embryol."},{"key":"B25","first-page":"881","article-title":"Divide, accumulate, differentiate: cell condensation in skeletal development revisited","volume":"39","author":"Hall","year":"1995","journal-title":"Int. J. Dev. Biol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1002\/(SICI)1521-1878(200002)22:2<138::AID-BIES5>3.0.CO;2-4","article-title":"All for one and one for all: condensations and the initiation of skeletal development","volume":"22","author":"Hall","year":"2000","journal-title":"Bioessays"},{"key":"B27","first-page":"319","article-title":"Chapter 19 - the membranous skeleton: Condensations","volume-title":"Bones and cartilage","author":"Hall","year":""},{"key":"B28","first-page":"333","article-title":"Chapter 20 - from condensation to differentiation","volume-title":"Bones and cartilage","author":"Hall","year":""},{"key":"B29","doi-asserted-by":"publisher","first-page":"2587","DOI":"10.1242\/dev.125.14.2587","article-title":"Hoxa-2 restricts the chondrogenic domain and inhibits bone formation during development of the branchial area","volume":"125","author":"Kanzler","year":"1998","journal-title":"Development"},{"key":"B30","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1038\/nature01654","article-title":"The complexities of skeletal biology","volume":"423","author":"Karsenty","year":"2003","journal-title":"Nature"},{"key":"B31","doi-asserted-by":"publisher","first-page":"e25902","DOI":"10.7554\/eLife.25902","article-title":"Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage","volume":"6","author":"Kaucka","year":"2017","journal-title":"Elife"},{"key":"B32","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.ydbio.2015.04.007","article-title":"Distinct effects of Hoxa2 overexpression in cranial neural crest populations reveal that the mammalian hyomandibular-ceratohyal boundary maps within the styloid process","volume":"402","author":"Kitazawa","year":"2015","journal-title":"Dev. Biol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"e26393","DOI":"10.1371\/journal.pone.0026393","article-title":"Presence of cartilage stem\/progenitor cells in adult mice auricular perichondrium","volume":"6","author":"Kobayashi","year":"2011","journal-title":"PLOS ONE"},{"key":"B34","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1016\/s0092-8674(00)80258-5","article-title":"Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts","volume":"89","author":"Komori","year":"1997","journal-title":"Cell"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.1002\/jcb.20958","article-title":"Regulation of osteoblast differentiation by transcription factors","volume":"99","author":"Komori","year":"2006","journal-title":"J. Cell. Biochem."},{"key":"B36","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1002\/aja.1002030204","article-title":"Syndecan-3, tenascin-C, and the development of cartilaginous skeletal elements and joints in chick limbs","volume":"203","author":"Koyama","year":"1995","journal-title":"Dev. Dyn."},{"key":"B37","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1002\/jor.1100140310","article-title":"Expression of syndecan-3 and tenascin-C: Possible involvement in periosteum development","volume":"14","author":"Koyama","year":"1996","journal-title":"J. Orthop. Res."},{"key":"B38","doi-asserted-by":"publisher","first-page":"2336","DOI":"10.1128\/mcb.17.4.2336","article-title":"SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene","volume":"17","author":"Lefebvre","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1096\/fj.201600918R","article-title":"Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice","volume":"31","author":"Li","year":"2017","journal-title":"FASEB J."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ydbio.2005.04.023","article-title":"Computational representation of developmental genetic regulatory networks","volume":"283","author":"Longabaugh","year":"2005","journal-title":"Dev. Biol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/j.bbagrm.2008.07.014","article-title":"Visualization, documentation, analysis, and communication of large-scale gene regulatory networks","volume":"1789","author":"Longabaugh","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"B42","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1002\/dvdy.24016","article-title":"Biochemistry of the tale transcription factors PREP, MEIS, and PBX in vertebrates","volume":"243","author":"Longobardi","year":"2014","journal-title":"Dev. Dyn."},{"key":"B43","first-page":"187","article-title":"The ossified hyoid apparatus morphology, interpretation, clinical and functional significance. Presentation of a rare case and highlights of the literature","volume":"166","author":"Lykaki","year":"1988","journal-title":"Anat. Anz."},{"key":"B44","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1016\/s0306-4522(02)00053-2","article-title":"Forebrain-specific promoter\/enhancer D6 derived from the mouse Dach1 gene controls expression in neural stem cells","volume":"112","author":"Machon","year":"2002","journal-title":"Neuroscience"},{"key":"B72","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s12861-015-0093-6","article-title":"Meis2 is essential for cranial and cardiac neural crest development","volume":"15","author":"Machon","year":"2015","journal-title":"BMC Dev. Biol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.devcel.2010.07.010","article-title":"Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels","volume":"19","author":"Maes","year":"2010","journal-title":"Dev. Cell"},{"key":"B46","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1101\/gad.9.10.1237","article-title":"The paired-like homeo box gene MHox is required for early events of skeletogenesis in multiple lineages","volume":"9","author":"Martin","year":"1995","journal-title":"Genes Dev."},{"key":"B47","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/j.ydbio.2009.07.004","article-title":"Pbx acts with Hand2 in early myocardial differentiation","volume":"333","author":"Maves","year":"2009","journal-title":"Dev. Biol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/0003-9969(87)90055-0","article-title":"The induction of odontogenesis in non-dental mesenchyme combined with early murine mandibular arch epithelium","volume":"32","author":"Mina","year":"1987","journal-title":"Arch. Oral Biol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"9360","DOI":"10.1073\/pnas.1631288100","article-title":"Sox9 is required for determination of the chondrogenic cell lineage in the cranial neural crest","volume":"100","author":"Mori-Akiyama","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B50","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1007\/s12024-015-9655-y","article-title":"The use of computed tomography in determining development, anomalies, and trauma of the hyoid bone","volume":"11","author":"Naimo","year":"2015","journal-title":"Forensic Sci. Med. Pathol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/j.devcel.2014.09.018","article-title":"HAND2 targets define a network of transcriptional regulators that compartmentalize the early limb bud mesenchyme","volume":"31","author":"Osterwalder","year":"2014","journal-title":"Dev. Cell"},{"key":"B52","doi-asserted-by":"publisher","first-page":"39","DOI":"10.12688\/f1000research.7620.1","article-title":"BioTapestry now provides a web application and improved drawing and layout tools [version 1; peer review: 3 approved","volume":"5","author":"Paquette","year":"2016","journal-title":"F1000Res."},{"key":"B53","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1038\/s41467-019-09197-8","article-title":"A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates","volume":"10","author":"Parker","year":"2019","journal-title":"Nat. Commun."},{"key":"B54","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.ydbio.2021.07.014","article-title":"Analysis of lamprey meis genes reveals that conserved inputs from Hox, Meis and Pbx proteins control their expression in the hindbrain and neural tube","volume":"479","author":"Parker","year":"2021","journal-title":"Dev. Biol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"667","DOI":"10.2298\/sarh0812667p","article-title":"Styloid syndrome: a review of literature","volume":"136","author":"Petrovi\u0107","year":"2008","journal-title":"Srp. Arh. Celok. Lek."},{"key":"B56","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s40851-019-0123-5","article-title":"Diminution of pharyngeal segmentation and the evolution of the amniotes","volume":"5","author":"Poopalasundaram","year":"2019","journal-title":"Zool. Lett."},{"key":"B57","doi-asserted-by":"publisher","first-page":"2523","DOI":"10.1101\/gad.9.20.2523","article-title":"Null mutation of Dlx-2 results in abnormal morphogenesis of proximal first and second branchial arch derivatives and abnormal differentiation in the forebrain","volume":"9","author":"Qiu","year":"1995","journal-title":"Genes Dev."},{"key":"B58","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1016\/0092-8674(93)90620-6","article-title":"A homeotic transformation is generated in the rostral branchial region of the head by disruption of Hoxa-2, which acts as a selector gene","volume":"75","author":"Rijli","year":"1993","journal-title":"Cell"},{"key":"B59","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1111\/j.1469-7580.2006.00524.x","article-title":"Morphogenesis of the second pharyngeal arch cartilage (Reichert\u2019s cartilage) in human embryos","volume":"208","author":"Rodr\u00edguez-V\u00e1zquez","year":"2006","journal-title":"J. Anat."},{"key":"B60","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1111\/j.1469-7580.2011.01387.x","article-title":"Human fetal hyoid body origin revisited","volume":"219","author":"Rodr\u00edguez-V\u00e1zquez","year":"2011","journal-title":"J. Anat."},{"key":"B61","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1002\/ar.23166","article-title":"The origin of the variations of the hyoid apparatus in human","volume":"298","author":"Rodr\u00edguez\u2010V\u00e1zquez","year":"2015","journal-title":"Anat. Rec."},{"key":"B62","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1002\/dvdy.10380","article-title":"Patterning of the hyoid cartilage depends upon signals arising from the ventral foregut endoderm","volume":"228","author":"Ruhin","year":"2003","journal-title":"Dev. Dyn."},{"key":"B63","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1023\/a:1003449525619","article-title":"Association of cell and substrate adhesion molecules with connexin43 during intramembranous bone formation","volume":"30","author":"Rundus","year":"1998","journal-title":"Histochem. J."},{"key":"B64","doi-asserted-by":"publisher","first-page":"20130726","DOI":"10.1098\/rsif.2013.0726","article-title":"Glassy dynamics in three-dimensional embryonic tissues","volume":"10","author":"Sch\u00f6tz","year":"2013","journal-title":"J. R. Soc. Interface"},{"key":"B65","doi-asserted-by":"publisher","first-page":"3543","DOI":"10.1242\/dev.128.18.3543","article-title":"Requirement for Pbx1 in skeletal patterning and programming chondrocyte proliferation and differentiation","volume":"128","author":"Selleri","year":"2001","journal-title":"Development"},{"key":"B66","first-page":"72","article-title":"[Variants of the structure of the stylohyoid complex","volume":"6","author":"Shul\u2019ga","year":"2006","journal-title":"Vestn. Otorinolaringol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"2929","DOI":"10.1242\/dev.128.15.2929","article-title":"Prx1 and Prx2 are upstream regulators of sonic hedgehog and control cell proliferation during mandibular arch morphogenesis","volume":"128","author":"ten Berge","year":"2001","journal-title":"Development"},{"key":"B68","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1016\/j.jcfm.2005.09.006","article-title":"Overview of the ossified stylohyoid ligament based in more than 1200 forensic autopsies","volume":"13","author":"Vougiouklakis","year":"2006","journal-title":"J. Clin. Forensic Med."},{"key":"B69","doi-asserted-by":"publisher","first-page":"5449","DOI":"10.1074\/jbc.RA120.012684","article-title":"The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice","volume":"295","author":"Wang","year":"2020","journal-title":"J. Biol. Chem."},{"key":"B70","doi-asserted-by":"publisher","first-page":"e2797","DOI":"10.3791\/2797","article-title":"Whole mount in situ hybridization of E8.5 to E11.5 mouse embryos","volume":"56","author":"Wei","year":"2011","journal-title":"J. Vis. Exp."},{"key":"B71","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1126\/science.aau9345","article-title":"New Jurassic mammaliaform sheds light on early evolution of mammal-like hyoid bones","volume":"365","author":"Zhou","year":"2019","journal-title":"Science"}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2022.951063\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T05:41:33Z","timestamp":1664343693000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2022.951063\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,28]]},"references-count":73,"alternative-id":["10.3389\/fcell.2022.951063"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2022.951063","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,28]]},"article-number":"951063"}}