{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:27:14Z","timestamp":1775017634852,"version":"3.50.1"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T00:00:00Z","timestamp":1684800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R03 DE028984 R15 DE026611"],"award-info":[{"award-number":["R03 DE028984 R15 DE026611"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> The pharyngeal arches are transient developmental structures that, in vertebrates, give rise to tissues of the head and neck. A critical process underlying the specification of distinct arch derivatives is segmentation of the arches along the anterior-posterior axis. Formation of ectodermal-endodermal interfaces is a key mediator of this process, and although it is essential, mechanisms regulating the establishment of these interfaces vary between pouches and between taxa.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Here, we focus on the patterning and morphogenesis of epithelia associated with the first pharyngeal arch, the first pharyngeal pouch (pp1) and the first pharyngeal cleft (pc1), and the role of <jats:italic>Fgf8<\/jats:italic> dosage in these processes in the mouse model system.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> We find that severe reductions of <jats:italic>Fgf8<\/jats:italic> levels disrupt both pp1 and pc1 development. Notably, out-pocketing of pp1 is largely robust to <jats:italic>Fgf8<\/jats:italic> reductions, however, pp1 extension along the proximal-distal axis fails when <jats:italic>Fgf8<\/jats:italic> is low. Our data indicate that <jats:italic>Fgf8<\/jats:italic> is required for specification of regional identity in both pp1 and pc1, for localized changes in cell polarity, and for elongation and extension of both pp1 and pc1.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> Based on <jats:italic>Fgf8<\/jats:italic>-mediated changes in tissue relationships between pp1 and pc1, we hypothesize that extension of pp1 requires physical interaction with pc1. Overall, our data indicate a critical role for the lateral surface ectoderm in segmentation of the first pharyngeal arch that has previously been under-appreciated.<\/jats:p>","DOI":"10.3389\/fcell.2023.1186526","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T04:47:22Z","timestamp":1684817242000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Fgf8 regulates first pharyngeal arch segmentation through pouch-cleft interactions"],"prefix":"10.3389","volume":"11","author":[{"given":"Nathaniel","family":"Zbasnik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Fish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,5,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/bs.ctdb.2015.11.023","article-title":"Organizers in development","volume":"117","author":"Anderson","year":"2016","journal-title":"Curr. 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