{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:10:58Z","timestamp":1789683058177,"version":"4.0.1"},"reference-count":74,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>The high risks of traumatic cataract treatments promoted the development of the concept of autologous lens regeneration. Biochemical cues can influence the cellular behavior of stem cells, and in this case, biophysical cues may be the important factors in producing rapid activation of cellular behavior. Here we bio-printed mesenchymal stem cells (MSCs) using a commonly used bioink sodium alginate-gelatin blends, and investigated the induction effect of MSC differentiation towards lens epithelial stem cells (LESCs) under a combination of biochemical cues and biophysical cues. It was found that biochemical cues in the porous three-dimensional (3D) matrix constructed using bioink sodium alginate-gelatin blends for bio-printing did not reduce the cell viability of loaded MSCs in the matrix by scanning electron microscope (SEM) observation and cell viability detection. Loaded MSCs in the matrix were consistently upregulated in the expression of proteins and genes involved in phenotypes and development signaling pathways of LESCs, as detected by polymerase chain reaction (PCR) with the support of biochemical cues. These results indicated that biophysical cues could rapidly activate the cellular behavior of MSCs differentiation, and biochemical cues could continuously induce MSCs differentiation towards LESCs.<\/jats:p>","DOI":"10.3389\/fcell.2024.1526943","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T07:06:48Z","timestamp":1736147208000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix"],"prefix":"10.3389","volume":"12","author":[{"given":"Yufan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuowei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianju","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.ajo.2004.10.011","article-title":"Fishing-related ocular trauma","volume":"139","author":"Alfaro","year":"2005","journal-title":"Am. J. Ophthalmol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"887","DOI":"10.2174\/156652409789105598","article-title":"Effects of alpha-crystallin on lens cell function and cataract pathology","volume":"9","author":"Andley","year":"2009","journal-title":"Curr. Mol. Med."},{"key":"B3","doi-asserted-by":"publisher","first-page":"3511","DOI":"10.1242\/dev.098681","article-title":"Notch\/Rbpj\u03ba signaling regulates progenitor maintenance and differentiation of hypothalamic arcuate neurons","volume":"140","author":"Aujla","year":"2013","journal-title":"Development"},{"key":"B4","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.preteyeres.2017.04.001","article-title":"The lens growth process","volume":"60","author":"Bassnett","year":"2017","journal-title":"Prog. Retin Eye Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1016\/j.bbrc.2016.08.068","article-title":"Lens transcriptome profile during cataract development in Mip-null mice","volume":"478","author":"Bennett","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B6","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.ydbio.2008.09.003","article-title":"Essential role of BMPs in FGF-induced secondary lens fiber differentiation","volume":"324","author":"Boswell","year":"2008","journal-title":"Dev. Biol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.21037\/atm-20-3033","article-title":"Recent developments in the management of congenital cataract","volume":"8","author":"Bremond-Gignac","year":"2020","journal-title":"Ann. Transl. Med."},{"key":"B8","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/j.ydbio.2008.07.002","article-title":"Differential requirement for beta-catenin in epithelial and fiber cells during lens development","volume":"321","author":"Cain","year":"2008","journal-title":"Dev. Biol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"11135","DOI":"10.1038\/ncomms11135","article-title":"Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling","volume":"7","author":"Carvajal-Gonzalez","year":"2016","journal-title":"Nat. Commun."},{"key":"B10","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/s13072-023-00478-7","article-title":"Dynamic changes in whole genome DNA methylation, chromatin and gene expression during mouse lens differentiation","volume":"16","author":"Chang","year":"2023","journal-title":"Epigenetics Chromatin"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1038\/cdd.2015.168","article-title":"Fate decision of mesenchymal stem cells: adipocytes or osteoblasts","volume":"23","author":"Chen","year":"2016","journal-title":"Cell Death Differ."},{"key":"B12","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1387\/ijdb.041882yc","article-title":"Expression of Frizzleds and secreted frizzled-related proteins (Sfrps) during mammalian lens development","volume":"48","author":"Chen","year":"2004","journal-title":"Int. J. Dev. Biol."},{"key":"B13","first-page":"319","article-title":"Lens protein gene expression: alpha-crystallins and MIP","volume":"8","author":"Chepelinsky","year":"1991","journal-title":"Lens Eye Toxic. Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"3516","DOI":"10.3390\/cells11213516","article-title":"Generation of lens progenitor cells and lentoid bodies from pluripotent stem cells: novel tools for human lens development and ocular disease etiology","volume":"11","author":"Cvekl","year":"2022","journal-title":"Cells"},{"key":"B15","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/j.exer.2009.04.012","article-title":"Retinoic acid signaling in mammalian eye development","volume":"89","author":"Cvekl","year":"2009","journal-title":"Exp. Eye Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.ydbio.2013.10.030","article-title":"Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism","volume":"385","author":"Dawes","year":"2014","journal-title":"Dev. Biol."},{"key":"B17","first-page":"76","article-title":"Specific interaction between lens MIP\/Aquaporin-0 and two members of the gamma-crystallin family","volume":"11","author":"Fan","year":"2005","journal-title":"Mol. Vis."},{"key":"B18","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1016\/j.semcdb.2008.11.015","article-title":"Retina and lens regeneration in anuran amphibians","volume":"20","author":"Filoni","year":"2009","journal-title":"Semin. Cell Dev. Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1038\/s41433-019-0723-5","article-title":"Capsular fibrosis: a review of prevention methods and management","volume":"34","author":"Fi\u0219u\u0219","year":"2020","journal-title":"Eye (Lond)."},{"key":"B20","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1007\/s11064-016-2156-x","article-title":"Lens biology is a dimension of neurobiology","volume":"42","author":"Frederikse","year":"2017","journal-title":"Neurochem. Res."},{"key":"B21","doi-asserted-by":"publisher","first-page":"3764","DOI":"10.1101\/gad.12.23.3764","article-title":"BMP4 is essential for lens induction in the mouse embryo","volume":"12","author":"Furuta","year":"1998","journal-title":"Genes Dev."},{"key":"B22","doi-asserted-by":"publisher","first-page":"RA155","DOI":"10.12659\/msm.883475","article-title":"Melanocyte stem cells: biology and current aspects","volume":"18","author":"Gola","year":"2012","journal-title":"Med. Sci. Monit."},{"key":"B23","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s13287-019-1185-1","article-title":"The potential and limitations of induced pluripotent stem cells to achieve wound healing","volume":"10","author":"Gorecka","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"B24","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1111\/j.1440-169X.2008.01005.x","article-title":"Determinative roles of FGF and Wnt signals in iris-derived lens regeneration in newt eye","volume":"50","author":"Hayashi","year":"2008","journal-title":"Dev. Growth Differ."},{"key":"B25","doi-asserted-by":"publisher","first-page":"119330","DOI":"10.1016\/j.gep.2023.119330","article-title":"The role of mip in the development of lens in zebrafish","volume":"49","author":"He","year":"2023","journal-title":"Gene Expr. Patterns"},{"key":"B26","doi-asserted-by":"publisher","first-page":"e2102087","DOI":"10.1002\/adhm.202102087","article-title":"Medical applications of porous biomaterials: features of porosity and tissue-specific implications for biocompatibility","volume":"11","author":"Hernandez","year":"2022","journal-title":"Adv. Healthc. Mater"},{"key":"B27","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.biomaterials.2017.11.030","article-title":"Biomaterials-based 3D cell printing for next-generation therapeutics and diagnostics","volume":"156","author":"Jang","year":"2018","journal-title":"Biomaterials"},{"key":"B28","doi-asserted-by":"publisher","first-page":"E10","DOI":"10.3171\/2018.12.FOCUS18602","article-title":"Stem cell therapies for acute spinal cord injury in humans: a review","volume":"46","author":"Jin","year":"2019","journal-title":"Neurosurg. Focus"},{"key":"B29","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.semcdb.2007.10.005","article-title":"Eye evolution: lens and cornea as an upgrade of animal visual system","volume":"19","author":"Jonasova","year":"2008","journal-title":"Semin. Cell Dev. Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"e001064","DOI":"10.1136\/bmjophth-2022-001064","article-title":"Pathogenic genetic variants identified in Australian families with paediatric cataract","volume":"7","author":"Jones","year":"2022","journal-title":"BMJ Open Ophthalmol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"100522","DOI":"10.1016\/j.mtbio.2022.100522","article-title":"Extracellular vesicle-loaded hydrogels for tissue repair and regeneration","volume":"18","author":"Ju","year":"2023","journal-title":"Mater Today Bio"},{"key":"B32","doi-asserted-by":"publisher","first-page":"dev182923","DOI":"10.1242\/dev.182923","article-title":"Evolution and development of complex eyes: a celebration of diversity","volume":"147","author":"Koenig","year":"2020","journal-title":"Development"},{"key":"B33","doi-asserted-by":"publisher","first-page":"52","DOI":"10.17691\/stm2021.13.2.06","article-title":"Biocompatibility and osseointegration of calcium phosphate-coated and non-coated titanium implants with various porosities","volume":"13","author":"Korytkin","year":"2021","journal-title":"Sovrem. Tekhnologii Med."},{"key":"B34","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1080\/02713683.2019.1681003","article-title":"The development, growth, and regeneration of the crystalline lens: a review","volume":"45","author":"Kumar","year":"2020","journal-title":"Curr. Eye Res."},{"key":"B35","first-page":"2436","article-title":"Molecular and structural analysis of genetic variations in congenital cataract","volume":"19","author":"Kumar","year":"2013","journal-title":"Mol. Vis."},{"key":"B36","doi-asserted-by":"publisher","first-page":"107872","DOI":"10.1016\/j.exer.2019.107872","article-title":"Molecular genetics of congenital cataracts","volume":"191","author":"Li","year":"2020","journal-title":"Exp. Eye Res."},{"key":"B37","doi-asserted-by":"publisher","first-page":"640388","DOI":"10.3389\/fcell.2021.640388","article-title":"Biophysical and biochemical cues of biomaterials guide mesenchymal stem cell behaviors","volume":"9","author":"Li","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1097\/ICU.0000000000000547","article-title":"Cataract surgery and lens implantation","volume":"30","author":"Li","year":"2019","journal-title":"Curr. Opin. Ophthalmol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"e2400310","DOI":"10.1002\/adma.202400310","article-title":"In situ rapid-formation sprayable hydrogels for challenging tissue injury management","volume":"36","author":"Li","year":"2024","journal-title":"Adv. Mater"},{"key":"B40","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1038\/nature17181","article-title":"Lens regeneration using endogenous stem cells with gain of visual function","volume":"531","author":"Lin","year":"2016","journal-title":"Nature"},{"key":"B41","doi-asserted-by":"publisher","first-page":"8417","DOI":"10.2147\/IJN.S339500","article-title":"3D printed gelatin\/sodium alginate hydrogel scaffolds doped with nano-attapulgite for bone tissue repair","volume":"16","author":"Liu","year":"2021","journal-title":"Int. J. Nanomedicine"},{"key":"B42","doi-asserted-by":"publisher","first-page":"111387","DOI":"10.1016\/j.msec.2020.111387","article-title":"The stiffness of hydrogel-based bioink impacts mesenchymal stem cells differentiation toward sweat glands in 3D-bioprinted matrix","volume":"118","author":"Liu","year":"2021","journal-title":"Mater Sci. Eng. C Mater Biol. Appl."},{"key":"B43","doi-asserted-by":"publisher","first-page":"tkaa029","DOI":"10.1093\/burnst\/tkaa029","article-title":"Stiffness-mediated mesenchymal stem cell fate decision in 3D-bioprinted hydrogels","volume":"8","author":"Liu","year":"2020","journal-title":"Burns Trauma"},{"key":"B44","doi-asserted-by":"publisher","first-page":"2808","DOI":"10.1101\/gad.9.22.2808","article-title":"BMP-7 is an inducer of nephrogenesis, and is also required for eye development and skeletal patterning","volume":"9","author":"Luo","year":"1995","journal-title":"Genes Dev."},{"key":"B45","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.joco.2018.11.005","article-title":"Updates on managements of pediatric cataract","volume":"31","author":"Mohammadpour","year":"2019","journal-title":"J. Curr. Ophthalmol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"114","DOI":"10.17305\/bjbms.2017.1745","article-title":"A silent mutation in human alpha-A crystallin gene in patients with age-related nuclear or cortical cataract","volume":"17","author":"Mynampati","year":"2017","journal-title":"Bosn. J. Basic Med. Sci."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1742","DOI":"10.3390\/pharmaceutics15061742","article-title":"Enhancing anticancer efficacy of chemotherapeutics using targeting ligand-functionalized synthetic antigen receptor-mesenchymal stem cells","volume":"15","author":"Nethi","year":"2023","journal-title":"Pharmaceutics"},{"key":"B48","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/pr.2017.252","article-title":"3D bioprinting using stem cells","volume":"83","author":"Ong","year":"2018","journal-title":"Pediatr. Res."},{"key":"B49","doi-asserted-by":"publisher","first-page":"044101","DOI":"10.1088\/1758-5090\/7\/4\/044101","article-title":"Three-dimensional bioprinting of embryonic stem cells directs highly uniform embryoid body formation","volume":"7","author":"Ouyang","year":"2015","journal-title":"Biofabrication"},{"key":"B50","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.biomaterials.2017.12.025","article-title":"Mesenchymal stem cell-macrophage crosstalk and bone healing","volume":"196","author":"Pajarinen","year":"2019","journal-title":"Biomaterials"},{"key":"B51","doi-asserted-by":"publisher","first-page":"20200344","DOI":"10.1098\/rsta.2020.0344","article-title":"Colonization versus encapsulation in cell-laden materials design: porosity and process biocompatibility determine cellularization pathways","volume":"379","author":"Parisi","year":"2021","journal-title":"Philos. Trans. A Math. Phys. Eng. Sci."},{"key":"B52","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/j.survophthal.2005.06.010","article-title":"Techniques of intraocular lens suspension in the absence of capsular\/zonular support","volume":"50","author":"Por","year":"2005","journal-title":"Surv. Ophthalmol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/j.ydbio.2009.08.027","article-title":"The type I BMP receptors, Bmpr1a and Acvr1, activate multiple signaling pathways to regulate lens formation","volume":"335","author":"Rajagopal","year":"2009","journal-title":"Dev. Biol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"338","DOI":"10.3928\/1542-8877-19980401-16","article-title":"Isolated posterior capsule rupture in blunt trauma: pathogenesis and management","volume":"29","author":"Rao","year":"1998","journal-title":"Ophthalmic Surg. Lasers"},{"key":"B55","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.ydbio.2008.06.002","article-title":"Notch signaling regulates growth and differentiation in the mammalian lens","volume":"321","author":"Rowan","year":"2008","journal-title":"Dev. Biol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1007\/s00417-014-2806-0","article-title":"Ameliorative effects of SkQ1 eye drops on cataractogenesis in senescence-accelerated OXYS rats","volume":"253","author":"Rumyantseva","year":"2015","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1016\/j.ydbio.2009.05.566","article-title":"Notch signaling is required for lateral induction of Jagged1 during FGF-induced lens fiber differentiation","volume":"332","author":"Saravanamuthu","year":"2009","journal-title":"Dev. Biol."},{"key":"B58","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.ydbio.2011.11.011","article-title":"Conditional ablation of the Notch2 receptor in the ocular lens","volume":"362","author":"Saravanamuthu","year":"2012","journal-title":"Dev. Biol."},{"key":"B59","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/s00417-007-0698-y","article-title":"Ocular injuries and visual status before and after their management in the tribal areas of Western India: a historical cohort study","volume":"246","author":"Shah","year":"2008","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.jcjo.2013.03.010","article-title":"Controversies in traumatic cataract classification and management: a review","volume":"48","author":"Shah","year":"2013","journal-title":"Can. J. Ophthalmol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1007\/s12010-014-0874-6","article-title":"Preparation, mass diffusion, and biocompatibility analysis of porous-channel controlled calcium-alginate-gelatin hybrid microbeads for in vitro culture of NSCs","volume":"173","author":"Song","year":"2014","journal-title":"Appl. Biochem. Biotechnol."},{"key":"B62","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1016\/j.stem.2018.05.013","article-title":"Regenerating eye tissues to preserve and restore vision","volume":"22","author":"Stern","year":"2018","journal-title":"Cell Stem Cell"},{"key":"B63","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/s0012-1606(03)00179-9","article-title":"A role for Wnt\/beta-catenin signaling in lens epithelial differentiation","volume":"259","author":"Stump","year":"2003","journal-title":"Dev. Biol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/s0886-3350(98)80213-7","article-title":"Posterior capsule rupture after blunt trauma","volume":"24","author":"Thomas","year":"1998","journal-title":"J. Cataract. Refract Surg."},{"key":"B65","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/s0886-3350(13)80580-9","article-title":"Pre-existing posterior capsule breaks from perforating ocular injuries","volume":"20","author":"Vajpayee","year":"1994","journal-title":"J. Cataract. Refract Surg."},{"key":"B66","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/s0039-6257(01)00195-3","article-title":"Management of posterior capsule tears","volume":"45","author":"Vajpayee","year":"2001","journal-title":"Surv. Ophthalmol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1007\/s13770-021-00429-x","article-title":"The biocompatibility of multi-source stem cells and gelatin-carboxymethyl chitosan-sodium alginate hybrid biomaterials","volume":"19","author":"Wang","year":"2022","journal-title":"Tissue Eng. Regen. Med."},{"key":"B68","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1007\/s11427-019-9826-7","article-title":"TNF-\u03b1 suppresses sweat gland differentiation of MSCs by reducing FTO-mediated m(6)A-demethylation of Nanog mRNA","volume":"63","author":"Wang","year":"2020","journal-title":"Sci. China Life Sci."},{"key":"B69","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.exer.2008.10.016","article-title":"Posterior capsule opacification","volume":"88","author":"Wormstone","year":"2009","journal-title":"Exp. Eye Res."},{"key":"B70","doi-asserted-by":"publisher","first-page":"eaau3442","DOI":"10.1126\/sciadv.aau3442","article-title":"Conjoined-network rendered stiff and tough hydrogels from biogenic molecules","volume":"5","author":"Xu","year":"2019","journal-title":"Sci. Adv."},{"key":"B71","doi-asserted-by":"publisher","first-page":"3274","DOI":"10.1096\/fj.10-157255","article-title":"Efficient generation of lens progenitor cells and lentoid bodies from human embryonic stem cells in chemically defined conditions","volume":"24","author":"Yang","year":"2010","journal-title":"FASEB J."},{"key":"B72","doi-asserted-by":"publisher","first-page":"045020","DOI":"10.1088\/1758-5090\/ab38ef","article-title":"Enzymatically degradable alginate\/gelatin bioink promotes cellular behavior and degradation in vitro and in vivo","volume":"11","author":"Yao","year":"2019","journal-title":"Biofabrication"},{"key":"B73","doi-asserted-by":"publisher","first-page":"eaaz1094","DOI":"10.1126\/sciadv.aaz1094","article-title":"Biochemical and structural cues of 3D-printed matrix synergistically direct MSC differentiation for functional sweat gland regeneration","volume":"6","author":"Yao","year":"2020","journal-title":"Sci. Adv."},{"key":"B74","doi-asserted-by":"publisher","first-page":"1958","DOI":"10.1161\/CIRCRESAHA.117.311080","article-title":"Induced pluripotent stem cells 10 Years later: for cardiac applications","volume":"120","author":"Yoshida","year":"2017","journal-title":"Circ. Res."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2024.1526943\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T07:06:52Z","timestamp":1736147212000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2024.1526943\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":74,"alternative-id":["10.3389\/fcell.2024.1526943"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2024.1526943","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,6]]},"article-number":"1526943"}}