{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:24:02Z","timestamp":1791048242879,"version":"4.1.0"},"reference-count":64,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The advanced maternal age (AMA) pregnancies escalate rapidly, which are frequently linked to higher risks of adverse outcomes. Advanced maternal age (AMA) placenta exhibited premature aging, presumably resulting in trophoblast dysfunction, inadequate placentation. However, the precise reasons and mechanisms of trophoblast aging in AMA placenta remain unclear, posing a significant limitation to provide effective guidance for prenatal healthcare in clinical settings. Notably, the organism shows heightened vulnerability to oxidative damage as it ages. YAP (Yes-associated protein) was reported to play a critical role in regulation of aging and resisting oxidative damage, yet these roles had not been elucidated in the placenta. Therefore, this study explored the relationship between trophoblast cell aging and oxidative injury and YAP in AMA pregnancy, which not only provided an insight into the mechanisms of trophoblast cell aging, but also provide valuable directions for healthcare during AMA pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, human term placentas were collected from AMA and normal pregnancies for the analysis of aging, oxidative damage and YAP level. HTR8\/SVneo cells were manipulated with (hydrogen peroxide) H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> to explore the effects of oxidative damage on trophoblast cell senescence and YAP levels. YAP expression in HTR8\/SVneo cells was manipulated to investigate its role in trophoblastic senescence and oxidative damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the control group, the AMA placenta exhibits increased aging biomarkers, which is coupled with an elevation in oxidative damage within placental trophoblast cells and a notable decline in YAP levels. Cellular experiments demonstrated that oxidative damage from H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> triggered trophoblast cell senescence and resulted in a reduction of YAP levels. Furthermore, employing molecular modification to silence YAP expression in these cells led to an induction of aging. Conversely, overexpressing YAP ameliorated both trophoblast cell aging and the associated DNA oxidative damage that arised from H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The decline of YAP in AMA pregnancy should be responsible for the increased oxidative injury and premature placenta aging, indicating that YAP plays a significant role in combating oxidative damage and delaying aging, thereby providing a new guidance for prenatal care in AMA pregnancies. Maintaining YAP levels or implementing anti-oxidative stress interventions could potentially mitigate the incidence of complications involved AMA pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcell.2025.1479960","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:39:10Z","timestamp":1737448750000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Placental trophoblast aging in advanced maternal age is related to increased oxidative damage and decreased YAP"],"prefix":"10.3389","volume":"13","author":[{"given":"Song","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihao","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baokang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyu","family":"Gou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"4212","DOI":"10.1093\/emboj\/cdg417","article-title":"Reversal of human cellular senescence: roles of the p53 and p16 pathways","volume":"22","author":"Beausejour","year":"2003","journal-title":"EMBO J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/j.atherosclerosis.2004.07.023","article-title":"LDL size, total antioxidant status and oxidised LDL in normal human pregnancy: a longitudinal study","volume":"177","author":"Belo","year":"2004","journal-title":"Atherosclerosis"},{"key":"B3","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1161\/HYPERTENSIONAHA.116.08041","article-title":"Different impact of essential hypertension on structural and functional age-related vascular changes","volume":"69","author":"Bruno","year":"2017","journal-title":"Hypertension"},{"key":"B4","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.jsgi.2004.03.003","article-title":"Placental oxidative stress: from miscarriage to preeclampsia","volume":"11","author":"Burton","year":"2004","journal-title":"J. Soc. Gynecol. Investig."},{"key":"B5","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1038\/nrm2233","article-title":"Cellular senescence: when bad things happen to good cells","volume":"8","author":"Campisi","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"49439","DOI":"10.1074\/jbc.M409153200","article-title":"Loss of proliferative capacity and induction of senescence in oxidatively stressed human fibroblasts","volume":"279","author":"Chen","year":"2004","journal-title":"J. Biol. Chem."},{"key":"B7","doi-asserted-by":"publisher","first-page":"e13417","DOI":"10.1111\/acel.13417","article-title":"Advanced maternal age causes premature placental senescence and malformation via dysregulated \u03b1-Klotho expression in trophoblasts","volume":"20","author":"Chen","year":"2021","journal-title":"Aging Cell"},{"key":"B8","doi-asserted-by":"publisher","first-page":"2356","DOI":"10.1101\/gad.227512.113","article-title":"Cell fusion induced by ERVWE1 or measles virus causes cellular senescence","volume":"27","author":"Chuprin","year":"2013","journal-title":"Genes Dev."},{"key":"B9","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.placenta.2018.06.307","article-title":"Evidence of oxidative stress-induced senescence in mature, post-mature and pathological human placentas","volume":"68","author":"Cindrova-Davies","year":"2018","journal-title":"Placenta"},{"key":"B10","doi-asserted-by":"publisher","first-page":"H387","DOI":"10.1152\/ajpheart.00045.2019","article-title":"Advanced maternal age and the impact on maternal and offspring cardiovascular health","volume":"317","author":"Cooke","year":"2019","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"2853","DOI":"10.1371\/journal.pbio.0060301","article-title":"Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor","volume":"6","author":"Coppe","year":"2008","journal-title":"PLoS Biol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.placenta.2017.01.116","article-title":"The role of cellular senescence in ageing of the placenta","volume":"52","author":"Cox","year":"2017","journal-title":"Placenta"},{"key":"B13","doi-asserted-by":"publisher","first-page":"5753","DOI":"10.1016\/j.febslet.2006.09.036","article-title":"Klotho RNAi induces premature senescence of human cells via a p53\/p21 dependent pathway","volume":"580","author":"de Oliveira","year":"2006","journal-title":"FEBS Lett."},{"key":"B14","doi-asserted-by":"publisher","first-page":"320","DOI":"10.3390\/cells13040320","article-title":"Unraveling histone loss in aging and senescence","volume":"13","author":"Dubey","year":"2024","journal-title":"Cells"},{"key":"B15","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.cger.2010.08.006","article-title":"The biology of aging and frailty","volume":"27","author":"Fedarko","year":"2011","journal-title":"Clin. Geriatr. Med."},{"key":"B16","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/j.placenta.2011.02.004","article-title":"Placental oxidative DNA damage and its repair in preeclamptic women with fetal growth restriction","volume":"32","author":"Fujimaki","year":"2011","journal-title":"Placenta"},{"key":"B17","doi-asserted-by":"publisher","first-page":"2652","DOI":"10.1016\/j.cub.2017.07.033","article-title":"Unmasking transcriptional heterogeneity in senescent cells","volume":"27","author":"Hernandez-Segura","year":"2017","journal-title":"Curr. Biol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.3727\/096368910X546562","article-title":"Cell contact accelerates replicative senescence of human mesenchymal stem cells independent of telomere shortening and p53 activation: roles of Ras and oxidative stress","volume":"20","author":"Ho","year":"2011","journal-title":"Cell Transpl."},{"key":"B19","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1161\/HYPERTENSIONAHA.107.107607","article-title":"Placental origins of preeclampsia: challenging the current hypothesis","volume":"51","author":"Huppertz","year":"2008","journal-title":"Hypertension"},{"key":"B20","doi-asserted-by":"publisher","first-page":"19017","DOI":"10.1073\/pnas.0908686106","article-title":"A perinuclear actin cap regulates nuclear shape","volume":"106","author":"Khatau","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"B21","doi-asserted-by":"publisher","first-page":"2123","DOI":"10.1038\/s41467-017-02217-5","article-title":"Nuclear lamin A\/C harnesses the perinuclear apical actin cables to protect nuclear morphology","volume":"8","author":"Kim","year":"2017","journal-title":"Nat. Commun."},{"key":"B22","doi-asserted-by":"publisher","first-page":"491","DOI":"10.3109\/14767058.2012.733766","article-title":"The severity of hypoxic changes and oxidative DNA damage in the placenta of early-onset preeclamptic women and fetal growth restriction","volume":"26","author":"Kimura","year":"2013","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"B23","doi-asserted-by":"publisher","first-page":"2463","DOI":"10.1101\/gad.1971610","article-title":"The essence of senescence","volume":"24","author":"Kuilman","year":"2010","journal-title":"Genes Dev."},{"key":"B24","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/j.repbio.2017.09.001","article-title":"The decline of pregnancy rate and abnormal uterine responsiveness of steroid hormones in aging mice","volume":"17","author":"Li","year":"2017","journal-title":"Reprod. Biol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"4219","DOI":"10.1093\/eurheartj\/ehae379","article-title":"TRAP1 drives smooth muscle cell senescence and promotes atherosclerosis via HDAC3-primed histone H4 lysine 12 lactylation","volume":"45","author":"Li","year":"2024","journal-title":"Eur. Heart J."},{"key":"B26","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1161\/HYPERTENSIONAHA.121.18363","article-title":"Impaired sphingosine-1-phosphate synthesis induces preeclampsia by deactivating trophoblastic YAP (Yes-Associated protein) through S1PR2 (Sphingosine-1-Phosphate receptor-2)-induced actin polymerizations","volume":"79","author":"Liao","year":"2022","journal-title":"Hypertension"},{"key":"B27","doi-asserted-by":"publisher","first-page":"6026","DOI":"10.26355\/eurrev_201907_18415","article-title":"SHH expression in placental tissues and trophoblast cell oxidative stress injury during preeclampsia","volume":"23","author":"Liao","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1186\/s12964-023-01371-2","article-title":"YAP-mediated trophoblast dysfunction: the common pathway underlying pregnancy complications","volume":"21","author":"Lin","year":"2023","journal-title":"Cell Commun. Signal"},{"key":"B29","doi-asserted-by":"publisher","first-page":"eadd8673","DOI":"10.1126\/science.add8673","article-title":"Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1","volume":"381","author":"Liu","year":"2023","journal-title":"Science"},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.preghy.2019.11.002","article-title":"Hippo-YAP1 signaling pathway and severe preeclampsia (sPE) in the Chinese population","volume":"19","author":"Liu","year":"2020","journal-title":"Pregnancy Hypertens."},{"key":"B31","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1038\/sj.neo.7900073","article-title":"Dial 9-1-1 for p53: mechanisms of p53 activation by cellular stress","volume":"2","author":"Ljungman","year":"2000","journal-title":"Neoplasia"},{"key":"B32","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/s00418-016-1435-6","article-title":"Placental aging and oxidation damage in a tissue micro-array model: an immunohistochemistry study","volume":"146","author":"Londero","year":"2016","journal-title":"Histochem Cell Biol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.cell.2022.11.001","article-title":"Hallmarks of aging: an expanding universe","volume":"186","author":"Lopez-Otin","year":"2023","journal-title":"Cell"},{"key":"B34","doi-asserted-by":"publisher","first-page":"1046","DOI":"10.1161\/HYPERTENSIONAHA.113.01892","article-title":"Spiral artery remodeling and trophoblast invasion in preeclampsia and fetal growth restriction: relationship to clinical outcome","volume":"62","author":"Lyall","year":"2013","journal-title":"Hypertension"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1602","DOI":"10.1080\/14767058.2017.1410793","article-title":"Maternal age over 40 years and pregnancy outcome: a hospital-based survey","volume":"32","author":"Marozio","year":"2019","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"B36","doi-asserted-by":"publisher","first-page":"13562","DOI":"10.1073\/pnas.2002630117","article-title":"Pivotal role of the transcriptional co-activator YAP in trophoblast stemness of the developing human placenta","volume":"117","author":"Meinhardt","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"B37","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/S1472-6483(10)61464-2","article-title":"Defective implantation and placentation: laying the blueprint for pregnancy complications","volume":"14","author":"Norwitz","year":"2007","journal-title":"Reprod. Biomed. Online"},{"key":"B38","doi-asserted-by":"publisher","first-page":"e2300759","DOI":"10.1002\/advs.202300759","article-title":"eIF3f mediates SGOC pathway reprogramming by enhancing deubiquitinating activity in colorectal cancer","volume":"10","author":"Pan","year":"2023","journal-title":"Adv. Sci. (Weinh)"},{"key":"B39","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1038\/ncb1024","article-title":"Oxygen sensitivity severely limits the replicative lifespan of murine fibroblasts","volume":"5","author":"Parrinello","year":"2003","journal-title":"Nat. Cell Biol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1161\/CIRCULATIONAHA.122.060454","article-title":"Loss of nuclear envelope integrity and increased oxidant production cause DNA damage in adult hearts deficient in PKP2: a molecular substrate of ARVC","volume":"146","author":"Perez-Hernandez","year":"2022","journal-title":"Circulation"},{"key":"B41","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/j.freeradbiomed.2018.06.016","article-title":"Adaptive homeostasis and the free radical theory of ageing","volume":"124","author":"Pomatto","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"B42","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1038\/s41575-021-00571-w","article-title":"Hippo signalling in the liver: role in development, regeneration and disease","volume":"19","author":"Russell","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"e97780","DOI":"10.15252\/embj.201797780","article-title":"dNTP metabolism links mechanical cues and YAP\/TAZ to cell growth and oncogene-induced senescence","volume":"37","author":"Santinon","year":"2018","journal-title":"EMBO J."},{"key":"B44","doi-asserted-by":"publisher","first-page":"811","DOI":"10.3389\/fendo.2019.00811","article-title":"Impact of oxidative stress on age-associated decline in oocyte developmental competence","volume":"10","author":"Sasaki","year":"2019","journal-title":"Front. Endocrinol. (Lausanne)"},{"key":"B45","doi-asserted-by":"publisher","first-page":"3315","DOI":"10.1038\/ncomms4315","article-title":"A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response","volume":"5","author":"Shao","year":"2014","journal-title":"Nat. Commun."},{"key":"B46","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1038\/s41586-022-04924-6","article-title":"YAP\/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING","volume":"607","author":"Sladitschek-Martens","year":"2022","journal-title":"Nature"},{"key":"B47","doi-asserted-by":"publisher","first-page":"2461","DOI":"10.1097\/HJH.0000000000002185","article-title":"Aspirin enhances trophoblast invasion and represses soluble fms-like tyrosine kinase 1 production: a putative mechanism for preventing preeclampsia","volume":"37","author":"Su","year":"2019","journal-title":"J. Hypertens."},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12653","article-title":"Oxidative stress, placental ageing-related pathologies and adverse pregnancy outcomes","volume":"77","author":"Sultana","year":"2017","journal-title":"Am. J. Reprod. Immunol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"S762","DOI":"10.1016\/j.ajog.2017.11.567","article-title":"Is there a role for placental senescence in the genesis of obstetric complications and fetal growth restriction?","volume":"218","author":"Sultana","year":"2018","journal-title":"Am. J. Obstet. Gynecol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.1177\/1933719117746784","article-title":"YAP is decreased in preeclampsia and regulates invasion and apoptosis of HTR-8\/SVneo","volume":"25","author":"Sun","year":"2018","journal-title":"Reprod. Sci."},{"key":"B51","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.freeradbiomed.2024.01.033","article-title":"Bisphenol A induces placental ferroptosis and fetal growth restriction via the YAP\/TAZ-ferritinophagy axis","volume":"213","author":"Sun","year":"2024","journal-title":"Free Radic. Biol. Med."},{"key":"B52","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s00428-003-0903-2","article-title":"Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction","volume":"444","author":"Takagi","year":"2004","journal-title":"Virchows Arch."},{"key":"B53","doi-asserted-by":"publisher","first-page":"10428","DOI":"10.1073\/pnas.0401424101","article-title":"Lamin B1 is required for mouse development and nuclear integrity","volume":"101","author":"Vergnes","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"B54","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.placenta.2022.08.062","article-title":"Exploration of fetal growth restriction induced by vitamin D deficiency in rats via Hippo-YAP signaling pathway","volume":"128","author":"Wang","year":"2022","journal-title":"Placenta"},{"key":"B55","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1038\/s41588-023-01647-w","article-title":"Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts identifies cellular trajectories during pregnancy","volume":"56","author":"Wang","year":"2024","journal-title":"Nat. Genet."},{"key":"B56","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1038\/s41467-017-00308-x","article-title":"Decidualisation and placentation defects are a major cause of age-related reproductive decline","volume":"8","author":"Woods","year":"2017","journal-title":"Nat. Commun."},{"key":"B57","doi-asserted-by":"publisher","first-page":"6239","DOI":"10.1038\/ncomms7239","article-title":"Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis","volume":"6","author":"Wu","year":"2015","journal-title":"Nat. Commun."},{"key":"B58","doi-asserted-by":"publisher","first-page":"1663","DOI":"10.1016\/j.celrep.2013.04.020","article-title":"The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense","volume":"3","author":"Wu","year":"2013","journal-title":"Cell Rep."},{"key":"B59","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1038\/s41467-023-37094-8","article-title":"Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence","volume":"14","author":"Wu","year":"2023","journal-title":"Nat. Commun."},{"key":"B60","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.ceb.2020.08.010","article-title":"Progress in deciphering trophoblast cell differentiation during human placentation","volume":"67","author":"Xiao","year":"2020","journal-title":"Curr. Opin. Cell Biol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"3615","DOI":"10.1158\/0008-5472.CAN-12-3793","article-title":"YAP\/TEAD-mediated transcription controls cellular senescence","volume":"73","author":"Xie","year":"2013","journal-title":"Cancer Res."},{"key":"B62","doi-asserted-by":"publisher","first-page":"e13491","DOI":"10.1111\/acel.13491","article-title":"Advanced maternal age-associated SIRT1 deficiency compromises trophoblast epithelial-mesenchymal transition through an increase in vimentin acetylation","volume":"20","author":"Xiong","year":"2021","journal-title":"Aging Cell"},{"key":"B63","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.phrs.2019.03.018","article-title":"Dysregulation of the Hippo pathway signaling in aging and cancer","volume":"143","author":"Yeung","year":"2019","journal-title":"Pharmacol. Res."},{"key":"B64","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/s12958-022-00909-2","article-title":"MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway","volume":"20","author":"Zang","year":"2022","journal-title":"Reprod. Biol. Endocrinol."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2025.1479960\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:39:14Z","timestamp":1737448754000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2025.1479960\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,21]]},"references-count":64,"alternative-id":["10.3389\/fcell.2025.1479960"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2025.1479960","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,21]]},"article-number":"1479960"}}