{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:52:41Z","timestamp":1791035561236,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021778","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>A key factor to the success of <jats:italic>in vitro<\/jats:italic> fertilization (IVF) is the preparation of human sperm, a critical step that directly impacts the efficacy of the procedure. This proof-of-concept study evaluated the effect of HyperSperm, a novel sperm preparation technique designed to enhance sperm function, on fertilization, embryo development, and pregnancy outcomes in both a mouse model and a first-in-human trial following IVF. In mice, HyperSperm treatment significantly increased hyperactivated motility (p &amp;lt; 0.05), leading to improved fertilization and blastocyst development (p &amp;lt; 0.05), as well as higher implantation rates (p &amp;lt; 0.05) and larger litter sizes (p &amp;lt; 0.05). Offspring displayed normal growth and fertility. In human sperm samples from normozoospermic men, HyperSperm exhibited a high safety profile, with motility, acrosome reaction, viability, and DNA fragmentation comparable to controls. A first-in-human, prospective, single-center, split-oocyte study in 10 couples undergoing IVF with donated oocytes demonstrated similar fertilization rates between HyperSperm and control groups (p = 0.425), but significantly higher usable blastocyst rates in the HyperSperm arm (43.8% vs. 67.9%, p = 0.0122). Morphokinetic parameters were comparable between groups. These results suggest that HyperSperm enhances sperm hyperactivation, a hallmark of capacitation, leading to improved embryo development in both mice and humans. This technique represents a promising approach to optimizing sperm preparation in assisted reproduction, warranting further clinical investigation.<\/jats:p>","DOI":"10.3389\/fcell.2025.1596421","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T10:37:12Z","timestamp":1747132632000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Increased reproductive outcomes after optimized sperm preparation"],"prefix":"10.3389","volume":"13","author":[{"given":"Mat\u00edas D.","family":"G\u00f3mez-El\u00edas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermina M.","family":"Luque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Oscoz-Susino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anal\u00eda G.","family":"Novero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olinda","family":"Briski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e9s","family":"K\u00e1sparas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s J.","family":"Steeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cintia","family":"Stival","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Lavolpe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanina","family":"Julianelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Geller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn","family":"Attie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Vassena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dar\u00edo","family":"Krapf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano G.","family":"Buffone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2325","DOI":"10.1242\/dev.136499","article-title":"The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm","volume":"143","author":"Alvau","year":"2016","journal-title":"Dev. 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