{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T07:13:29Z","timestamp":1784963609904,"version":"3.55.0"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-15-00308"],"award-info":[{"award-number":["24-15-00308"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In recent years, there has been a significant increase in interest in <jats:italic>Acomys cahirinus<\/jats:italic> due to their unique regenerative properties and specific metabolism. We propose that <jats:italic>Acomys sp<\/jats:italic>. adipose-derived stem cells (ADSC) may have unique properties allowing them to adapt to caloric overload and prevent severe metabolic abnormalities. ADSC characterization from <jats:italic>Acomys cahirinus<\/jats:italic> may reveal novel pro-regenerative targets and provide insight into mechanisms enabling the maintenance of metabolic health during obesity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ADSCs were isolated from the subcutaneous fat depots of <jats:italic>Acomys cahirinus<\/jats:italic> and <jats:italic>Mus musculus,<\/jats:italic> which was used as a classic lab animal rodent model. The mesenchymal phenotype of ADSC was confirmed by surface markers expression and differentiation ability. Proliferation and migration of ADSC were assessed by metabolic tests and microscopy. Osteogenesis and adipogenesis were evaluated by specific staining and RT PCR gene expression analysis. Subcutaneous adipose tissue was characterized by histology and Western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Acomys cahirinus<\/jats:italic> ADSC exhibited classic mesenchymal phenotype. Proliferation and wound healing were more active in <jats:italic>Acomys cahirinus<\/jats:italic> ADSC. These ADSC demonstrated enhanced osteogenesis and suppressed adipogenesis. <jats:italic>Acomys cahirinus<\/jats:italic> has larger adipose tissue depots than <jats:italic>Mus musculus<\/jats:italic> and lower blood glucose level. <jats:italic>Acomys cahirinus<\/jats:italic> adipose tissue is distinguished by lowered proliferation, enlarged adipocytes and suppressed adipose tissue triglyceride lipase (ATGL) expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that <jats:italic>Acomys cahirinus<\/jats:italic> ADSC have high regenerative potential. Nevertheless, the augmented osteogenic capacity of <jats:italic>Acomys cahirinus<\/jats:italic> ADSC can be related with a limited ADSC participation in adipose tissue self-renewal. The reduction in ATGL expression observed in <jats:italic>Acomys cahirinus<\/jats:italic> adipose tissue may serve as a protective mechanism in the face of excessive adipose tissue accumulation.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcell.2025.1603405","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T05:30:07Z","timestamp":1753853407000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Osteogenic shift in the adipose-derived stem cells of Acomys cahirinus is linked to impaired adipose tissue self-renewal"],"prefix":"10.3389","volume":"13","author":[{"given":"M.","family":"Boldyreva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Zubkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Trubkina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Agareva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Michurina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Alekseeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Beloglazova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Ratner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Parfyonova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Stafeev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/life12050688","article-title":"Type 2 diabetes mellitus facilitates shift of adipose-derived stem cells ex vivo differentiation toward osteogenesis among patients with obesity","volume":"12","author":"Agareva","year":"2022","journal-title":"Life"},{"key":"B2","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1097\/01.GOX.0000992600.40109.24","article-title":"Therapeutic consistency of ADSC secretomes for wound healing, scar modulation, and autoimmune disease","volume":"11","author":"Alaniz","year":"2023","journal-title":"Plast. Reconstr. Surg. Glob. Open."},{"key":"B3","doi-asserted-by":"publisher","first-page":"3405","DOI":"10.1016\/j.febslet.2007.06.041","article-title":"Cell-surface H+-ATP synthase as a potential molecular target for anti-obesity drugs","volume":"581","author":"Arakaki","year":"2007","journal-title":"Febs. Lett."},{"key":"B4","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1089\/scd.2012.0346","article-title":"Comprehensive phenotypic characterization of human adipose-derived stromal\/stem cells and their subsets by a high throughput technology","volume":"22","author":"Baer","year":"2013","journal-title":"Stem. Cells. Dev."},{"key":"B5","doi-asserted-by":"publisher","first-page":"3088","DOI":"10.3390\/ijms20123088","article-title":"Transplantation of adipose stromal cell sheet producing hepatocyte growth factor induces pleiotropic effect in ischemic skeletal muscle","volume":"20","author":"Boldyreva","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"B6","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1111\/wrr.12385","article-title":"Cellular events during scar-free skin regeneration in the spiny mouse, acomys","volume":"24","author":"Brant","year":"2016","journal-title":"Wound. Repair. Regen."},{"key":"B7","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/0167-4943(91)90021-h","article-title":"Recent progress in testing the longevity determinant and dysdifferentiation hypotheses of aging","volume":"12","author":"Cutler","year":"1991","journal-title":"Arch. Gerontol. Geriatr."},{"key":"B8","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1007\/s10616-012-9443-4","article-title":"The isolation and characterization of putative mesenchymal stem cells from the spiny mouse","volume":"64","author":"Dickinson","year":"2012","journal-title":"Cytotechnology"},{"key":"B9","doi-asserted-by":"publisher","first-page":"e0280169","DOI":"10.1371\/journal.pone.0280169","article-title":"Generation and characterization of two immortalized dermal fibroblast cell lines from the spiny mouse (acomys)","volume":"18","author":"Dill","year":"2023","journal-title":"PLoS.One"},{"key":"B10","doi-asserted-by":"publisher","first-page":"8447","DOI":"10.1038\/s41598-017-09115-2","article-title":"Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells","volume":"7","author":"Doi","year":"2017","journal-title":"Sci. Rep."},{"key":"B11","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41536-020-00111-1","article-title":"Spiny mouse (acomys): an emerging research organism for regenerative medicine with applications beyond the skin","volume":"6","author":"Gaire","year":"2021","journal-title":"NPJ.Regen. Med."},{"key":"B13","doi-asserted-by":"publisher","first-page":"900","DOI":"10.2337\/db13-0436","article-title":"FABP4 attenuates PPAR\u03b3 and adipogenesis and is inversely correlated with PPAR\u03b3 in adipose tissues","volume":"63","author":"Garin-Shkolnik","year":"2014","journal-title":"Diabetes"},{"key":"B14","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.yjmcc.2015.02.012","article-title":"Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model","volume":"81","author":"Gautam","year":"2015","journal-title":"J. Mol. Cell. Cardiol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"11164","DOI":"10.1038\/ncomms11164","article-title":"Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals","volume":"7","author":"Gawriluk","year":"2016","journal-title":"Nat. Commun."},{"key":"B16","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1186\/s13287-023-03264-0","article-title":"Mesenchymal stromal cell-derived secretome-based therapy for neurodegenerative diseases: overview of clinical trials","volume":"14","author":"Ghasemi","year":"2023","journal-title":"Stem. Cell. Res. Ther."},{"key":"B17","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1007\/BF01257907","article-title":"Obesity and diabetes mellitus with striking congenital hyperplasia of the islets of Langerhans in spiny mice (Acomys cahirinus): I. Histological findings and preliminary metabolic observations","volume":"1","author":"Gonet","year":"1966","journal-title":"Diabetologia"},{"key":"B18","doi-asserted-by":"publisher","first-page":"777","DOI":"10.2337\/diab.28.8.777","article-title":"Effect of diet-induced obesity on glucose and insulin tolerance of a rodent with a low insulin response (Acomys cahirinus)","volume":"28","author":"Gutzeit","year":"1979","journal-title":"Diabetes, Eff. diet-induced Obes. glucose insulin Toler. a rodent a low insulin response (Acomys cahirinus) Diabetes"},{"key":"B19","first-page":"9","article-title":"The biology and husbandry of the african spiny mouse (Acomys cahirinus) and the research uses of a laboratory colony","volume":"55","author":"Haughton","year":"2016","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"B20","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1111\/exd.13899","article-title":"Comparative regenerative biology of spiny (Acomys cahirinus) and laboratory (Mus musculus) mouse skin","volume":"28","author":"Jiang","year":"2019","journal-title":"Exp. Dermatol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"C375","DOI":"10.1152\/ajpcell.00379.2020","article-title":"Adipose tissue inflammation and metabolic dysfunction in obesity","volume":"320","author":"Kawai","year":"2021","journal-title":"Am. J. Physiol. Cell. Physiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"E775","DOI":"10.1210\/jc.2011-2649","article-title":"Link between adipose tissue angiogenesis and fat accumulation in severely obese subjects","volume":"97","author":"Lemoine","year":"2012","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"B23","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1186\/s13287-017-0743-7","article-title":"Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats","volume":"8","author":"Liao","year":"2017","journal-title":"Stem. Cell. Res. Ther."},{"key":"B24","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1359\/jbmr.081257","article-title":"Restoration of bone mass and strength in glucocorticoid-treated mice by systemic transplantation of CXCR4 and cbfa-1 co-expressing mesenchymal stem cells","volume":"24","author":"Lien","year":"2009","journal-title":"J. Bone. Mineral. Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.3892\/mmr.2016.4768","article-title":"Autologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy","volume":"13","author":"Liu","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"B26","doi-asserted-by":"publisher","first-page":"8920","DOI":"10.1038\/s41598-018-27178-7","article-title":"Perfect chronic skeletal muscle regeneration in adult spiny mice, Acomys cahirinus","volume":"8","author":"Maden","year":"2018","journal-title":"Sci. Rep."},{"key":"B27","doi-asserted-by":"publisher","first-page":"dev167718","DOI":"10.1242\/dev.167718","article-title":"Model systems for regeneration: the spiny mouse, Acomys cahirinus","volume":"147","author":"Maden","year":"2020","journal-title":"Development"},{"key":"B28","doi-asserted-by":"publisher","first-page":"8996","DOI":"10.1038\/s41598-017-09334-7","article-title":"De novo transcriptome assembly for the spiny mouse (Acomys cahirinus)","volume":"7","author":"Mamrot","year":"2017","journal-title":"Sci. Rep."},{"key":"B37","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1002\/reg2.50","article-title":"Ear wound regeneration in the African spiny mouse Acomys cahirinus","volume":"3","author":"Matias Santos","year":"2016","journal-title":"Regen. (Oxf)"},{"key":"B29","doi-asserted-by":"publisher","first-page":"412","DOI":"10.2337\/db20-1306","article-title":"A cycle of inflammatory adipocyte death and regeneration in murine adipose tissue","volume":"71","author":"Monji","year":"2022","journal-title":"Diabetes"},{"key":"B30","doi-asserted-by":"publisher","first-page":"6473279","DOI":"10.1155\/2020\/6473279","article-title":"Effect of age and lipoperoxidation in rat and human adipose tissue-derived stem cells","volume":"2020","author":"Mu\u00f1oz","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"B31","doi-asserted-by":"publisher","first-page":"3497","DOI":"10.3390\/ijms19113497","article-title":"A comparison of proteins expressed between human and mouse adipose-derived mesenchymal stem cells by a proteome analysis through liquid chromatography with tandem mass spectrometry","volume":"19","author":"Nahar","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"B32","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1016\/j.devcel.2021.12.008","article-title":"Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection","volume":"57","author":"Nogueira-Rodrigues","year":"2022","journal-title":"Dev. Cell."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s40883-018-0068-9","article-title":"CD140b (PDGFR\u03b2) signaling in adipose-derived stem cells mediates angiogenic behavior of retinal endothelial cells","volume":"5","author":"Periasamy","year":"2018","journal-title":"Regen. Eng. Transl. Med."},{"key":"B34","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.ijcard.2021.06.015","article-title":"Functional heart recovery in an adult mammal, the spiny mouse","volume":"338","author":"Qi","year":"2021","journal-title":"Int. J. Cardiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1186\/s13287-019-1479-3","article-title":"A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery","volume":"10","author":"Sagaradze","year":"2019","journal-title":"Stem. Cell. Res. Ther."},{"key":"B36","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.gde.2020.05.019","article-title":"Regeneration in the spiny mouse, acomys, a new mammalian model","volume":"64","author":"Sandoval","year":"2020","journal-title":"Curr. Opin. Genet. Dev."},{"key":"B38","doi-asserted-by":"publisher","first-page":"14859","DOI":"10.1038\/ncomms14859","article-title":"Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice","volume":"8","author":"Schweiger","year":"2017","journal-title":"Nat. Commun."},{"key":"B39","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/nature11499","article-title":"Skin shedding and tissue regeneration in African spiny mice (Acomys)","volume":"489","author":"Seifert","year":"2012","journal-title":"Nature, Skin shedding tissue Regen. Afr. spiny mice (Acomys)"},{"key":"B40","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1002\/(sici)1520-7560(200003\/04)16:2<94::aid-dmrr82>3.0.co;2-u","article-title":"Overnutrition in spiny mice (Acomys cahirinus): \u03b2-cell expansion leading to rupture and overt diabetes on fat-rich diet and protective energy-wasting elevation in thyroid hormone on sucrose-rich diet","volume":"16","author":"Shafrir","year":"2000","journal-title":"Diabetes. Metab. Res. Rev."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1439","DOI":"10.1016\/0020-711x(83)90076-9","article-title":"Enzymatic and metabolic responses to affluent diet of two diabetes-prone species of spiny mice: acomys cahirinus and Acomys russatus","volume":"15","author":"Shafrir","year":"1983","journal-title":"Int. J. Biochem."},{"key":"B42","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1093\/ilar.47.3.212","article-title":"Nutritionally induced diabetes in desert rodents as models of type 2 diabetes: acomys cahirinus (spiny mice) and Psammomys obesus (desert gerbil)","volume":"47","author":"Shafrir","year":"2006","journal-title":"ILAR J."},{"key":"B43","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1080\/14712598.2016.1181170","article-title":"Adipose tissue-derived therapeutic cells for peripheral artery diseases: the fatty blessing","volume":"16","author":"Silvestre","year":"2016","journal-title":"Expert. Opin. Biol. Ther."},{"key":"B44","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1186\/s10020-020-00192-0","article-title":"Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders","volume":"26","author":"Skubis-Sikora","year":"2020","journal-title":"Mol. Med."},{"key":"B45","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/j.jdiacomp.2018.10.011","article-title":"Low proliferative potential of adipose-derived stromal cells associates with hypertrophy and inflammation in subcutaneous and omental adipose tissue of patients with type 2 diabetes mellitus J.Diabetes","volume":"33","author":"Stafeev","year":"2019","journal-title":"Complications"},{"key":"B46","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1002\/cne.24836","article-title":"Molecular and histologic outcomes following spinal cord injury in spiny mice, Acomys cahirinus","volume":"528","author":"Streeter","year":"2019","journal-title":"Acomys cahirinus"},{"key":"B47","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1002\/cyto.a.23239","article-title":"Mammalian MSC from selected species: features and applications","volume":"93","author":"Uder","year":"2018","journal-title":"Cytom. A"},{"key":"B48","doi-asserted-by":"publisher","first-page":"bio060565","DOI":"10.1242\/bio.060565","article-title":"Older spiny mice (Acomys cahirinus) have delayed and spatially heterogenous ear wound regeneration","volume":"13","author":"Varholick","year":"2024","journal-title":"Biol. Open."},{"key":"B49","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.1134\/S0006297919110099","article-title":"Latent inflammation and defect in adipocyte renewal as a mechanism of obesity-associated insulin resistance","volume":"84","author":"Vorotnikov","year":"2019","journal-title":"Biochem. (Mosc)"},{"key":"B50","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1677\/JOE-09-0026","article-title":"Degradation of IRS1 leads to impaired glucose uptake in adipose tissue of the type 2 diabetes mouse model TALLYHO\/Jng","volume":"203","author":"Wang","year":"2009","journal-title":"J. Endocrinol."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2025.1603405\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T05:30:08Z","timestamp":1753853408000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2025.1603405\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,30]]},"references-count":50,"alternative-id":["10.3389\/fcell.2025.1603405"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2025.1603405","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,30]]},"article-number":"1603405"}}