{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T02:09:09Z","timestamp":1784945349599,"version":"3.55.0"},"reference-count":75,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>\n                    Global male infertility, characterized by decreased spermatogenesis and sperm quality, is a significant concern. L-cysteine, essential for hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) synthesis, offers numerous biological advantages. However, the protective mechanisms of L-cysteine in treating spermatogenic dysfunction need further exploration. This study aims to examine L-cysteine\u2019s protective effects on busulfan-induced testicular toxicity. Results show that administering L-cysteine at different doses (2.5, 5.0, and 10 mg\/kg) led to notable improvements in final body weight, testis weight, sperm count, sperm motility, testosterone levels, and seminiferous tubule architecture. At a 5.0 mg\/kg dosage, L-cysteine mitigated testicular injury by activating the CBS\/H\n                    <jats:sub>2<\/jats:sub>\n                    S axis. Moreover, L-cysteine effectively reduced apoptosis and oxidative stress through Nrf2\/HO-1 pathway activation. Various analyses demonstrated that L-cysteine enhanced the repair of the blood-testis barrier (BTB) disrupted by busulfan. Mechanistically, L-cysteine activated the PI3K\/Akt\/mTOR pathway in the testes. Notably, the CBS inhibitor AOAA reversed L-cysteine\u2019s protective effects on busulfan-induced testicular damage. In summary, the study suggests that L-cysteine can safeguard against busulfan-induced spermatogenic dysfunction, apoptosis, oxidative stress, and BTB disruption by modulating the PI3K\/Akt\/mTOR pathway, hinging on CBS\/H\n                    <jats:sub>2<\/jats:sub>\n                    S axis activation. These findings propose L-cysteine as a potential treatment for male infertility, particularly in individuals undergoing busulfan chemotherapy.\n                  <\/jats:p>","DOI":"10.3389\/fcell.2025.1679330","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T05:36:16Z","timestamp":1761197776000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["L-cysteine mitigates busulfan-induced testicular injury through modulation of CBS\/H2S axis"],"prefix":"10.3389","volume":"13","author":[{"given":"Song","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anran","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianbing","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunling","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1080\/01480545.2020.1803905","article-title":"The protective effect of rutin against busulfan-induced testicular damage in adult rats","volume":"45","author":"Abarikwu","year":"","journal-title":"Drug Chem. 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