{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T11:15:42Z","timestamp":1771931742467,"version":"3.50.1"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Carcinoma (PTC) is the most common malignant tumor derived from thyroid follicular cells and represents the most common pathological type of thyroid malignancy. Cancer metastasis and radiosensitivity are important factors that limit the treatment of PTC. This study aimed to investigate the regulatory effects of transient receptor potential channel C5 (TrpC5) on the proliferation, invasion, migration, and radiosensitivity of PTC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human papillary thyroid carcinoma cell lines TPC-1 and B-CPAP were transfected with TrpC5 siRNA, pcDNA-TrpC5, or their corresponding negative control. PTC cells were stimulated by radiotherapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed that TrpC5 silencing weakened the proliferation, invasion, and migration of PTC cells, whereas TrpC5 overexpression promoted these cellular behaviors. Moreover, TrpC5 expression was progressively upregulated in PTC cells following exposure to irradiation (IR). TrpC5 silencing enhanced radiosensitivity of TPC-1 and B-CPAP cells. In addition, TrpC5 silencing enhanced the expression of DNA damage-related proteins p-ATM, p-CHK, and \u03b3H2AX in PTC cells under IR treatment. Overall, TrpC5 silencing weakened cell invasion and migration and enhanced the radiosensitivity of PTC cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings suggest that TrpC5 may serve as a potential therapeutic target for PTC and warrant further investigation\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1705616","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:39:04Z","timestamp":1770964744000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["TrpC5 silencing reduces cell invasion and migration and enhances radiosensitivity in papillary thyroid carcinoma cells"],"prefix":"10.3389","volume":"14","author":[{"given":"Jing","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Laboratory of Thyroid and Parathyroid Diseases, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zijiang","family":"Sang","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, People\u2019s Hospital of Qinghai Province","place":["Qinghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haibing","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lvzhou","family":"Cao","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxia","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rixiang","family":"Gong","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Laboratory of Thyroid and Parathyroid Diseases, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihui","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Laboratory of Thyroid and Parathyroid Diseases, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanping","family":"Gong","sequence":"additional","affiliation":[{"name":"Division of Thyroid Surgery, Department of General Surgery, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Laboratory of Thyroid and Parathyroid Diseases, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"727864","DOI":"10.3389\/fmolb.2021.727864","article-title":"High CTC-TRPC5 expression significantly associated with poor prognosis in radical resected colorectal cancer patients","volume":"8","author":"Cai","year":"2021","journal-title":"Front. Molecular Biosciences"},{"key":"B2","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1042\/CS20160759","article-title":"TrpC5 regulates differentiation through the Ca2+\/Wnt5a signalling pathway in colorectal cancer","volume":"131","author":"Chen","year":"","journal-title":"Clin. Science Lond. Engl. 1979"},{"key":"B3","doi-asserted-by":"publisher","first-page":"2439","DOI":"10.1042\/CS20171069","article-title":"Overexpression of TrpC5 promotes tumor metastasis via the HIF-1\u03b1-Twist signaling pathway in colon cancer","volume":"131","author":"Chen","year":"","journal-title":"Clin. Science Lond. Engl. 1979"},{"key":"B4","doi-asserted-by":"publisher","first-page":"3112","DOI":"10.1007\/s12325-020-01391-1","article-title":"Papillary thyroid cancer-aggressive variants and impact on management: a narrative review","volume":"37","author":"Coca-Pelaz","year":"2020","journal-title":"Adv. Therapy"},{"key":"B5","doi-asserted-by":"publisher","first-page":"2512","DOI":"10.1016\/j.bbamem.2015.06.009","article-title":"Plasma membrane calcium channels in cancer: alterations and consequences for cell proliferation and migration","volume":"1848","author":"D\u00e9liot","year":"2015","journal-title":"Biochimica Biophysica Acta"},{"key":"B6","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.cellsig.2017.03.005","article-title":"Roles for RACK1 in cancer cell migration and invasion","volume":"35","author":"Duff","year":"2017","journal-title":"Cell. Signalling"},{"key":"B7","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1038\/s41392-020-0150-x","article-title":"DNA damage response signaling pathways and targets for radiotherapy sensitization","volume":"5","author":"Huang","year":"2020","journal-title":"Cancer"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10090331","article-title":"TRPC6 channels are required for proliferation, migration and invasion of breast cancer cell lines by modulation of Orai1 and Orai3 surface exposure","volume":"10","author":"Jardin","year":"2018","journal-title":"Cancers (Basel)."},{"key":"B9","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1667\/RR1844.1","article-title":"The role of the DNA damage response mechanisms after low-dose radiation exposure and a consideration of potentially sensitive individuals","volume":"174","author":"Jeggo","year":"2010","journal-title":"Radiat. Research"},{"key":"B10","doi-asserted-by":"publisher","first-page":"154","DOI":"10.5455\/medarh.2021.75.154-157","article-title":"Ectopic thyroid papillary carcinoma with cervical lymph node metastasis as the initial presentation, accompanied by benign thyroid gland","volume":"75","author":"Kola","year":"2021","journal-title":"Med. Archives (Sarajevo, Bosnia Herzegovina)"},{"key":"B11","first-page":"e23372","article-title":"Clemizole hydrochloride, a potent TRPC5 calcium channel inhibitor, prevents cisplatin-induced nephrotoxicity in spraque-dawley rats","volume":"37","author":"Kuma\u015f-Kulualp","year":"2023","journal-title":"arXiv"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1158\/1541-7786.MCR-10-0162","article-title":"Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through met inhibition in papillary thyroid cancer","volume":"8","author":"Lin","year":"2010","journal-title":"Mol. Cancer Research MCR"},{"key":"B13","unstructured":"Breast cancer research and treatment\n          \n          \n            \n              Liu\n              L. J.\n            \n          \n          \n          2024"},{"key":"B14","doi-asserted-by":"publisher","first-page":"268","DOI":"10.2174\/1574892814666190919145453","article-title":"Association between hTERT polymorphisms and female papillary thyroid carcinoma","volume":"14","author":"Liu","year":"2019","journal-title":"Recent Patents Anti-cancer Drug Discovery"},{"key":"B15","doi-asserted-by":"publisher","first-page":"662","DOI":"10.4161\/cc.9.4.10764","article-title":"gammaH2AX foci analysis for monitoring DNA double-strand break repair: strengths, limitations and optimization","volume":"9","author":"L\u00f6brich","year":"2010","journal-title":"Cell CycleGeorget. Tex"},{"key":"B16","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1586\/14737140.5.2.333","article-title":"New opportunities in chemosensitization and radiosensitization: modulating the DNA-Damage response","volume":"5","author":"Luo","year":"2005","journal-title":"Expert Review Anticancer Therapy"},{"key":"B17","doi-asserted-by":"publisher","first-page":"16282","DOI":"10.1073\/pnas.1202989109","article-title":"Transient receptor potential channel TRPC5 is essential for P-glycoprotein induction in drug-resistant cancer cells","volume":"109","author":"Ma","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B18","doi-asserted-by":"publisher","first-page":"2411","DOI":"10.3892\/or.2014.3483","article-title":"Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo","volume":"32","author":"Meng","year":"2014","journal-title":"Oncol. Reports"},{"key":"B19","doi-asserted-by":"publisher","first-page":"2898","DOI":"10.1158\/1078-0432.CCR-13-3229","article-title":"Molecular pathways: overcoming radiation resistance by targeting DNA damage response pathways","volume":"21","author":"Morgan","year":"2015","journal-title":"Clin. Cancer Research An Official Journal Am. Assoc. Cancer Res."},{"key":"B20","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1007\/s12576-016-0480-1","article-title":"Transient receptor potential cation 3 channel regulates melanoma proliferation and migration","volume":"67","author":"Oda","year":"2017","journal-title":"J. Physiol. Sci."},{"key":"B21","doi-asserted-by":"publisher","first-page":"768","DOI":"10.3332\/ecancer.2017.768","article-title":"Proteins regulating the intercellular transfer and function of P-glycoprotein in multidrug-resistant cancer","volume":"11","author":"Pokharel","year":"2017","journal-title":"Ecancermedicalscience"},{"key":"B22","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1152\/physrev.00044.2016","article-title":"Ion channels in cancer: are cancer hallmarks oncochannelopathies?","volume":"98","author":"Prevarskaya","year":"2018","journal-title":"Physiol. Reviews"},{"key":"B23","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.canlet.2018.09.011","article-title":"RNA interference to enhance radiation therapy: targeting the DNA damage response","volume":"439","author":"Ratnayake","year":"2018","journal-title":"Cancer Letters"},{"key":"B24","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1111\/cyt.12772","article-title":"One of the many faces of papillary thyroid carcinoma","volume":"31","author":"Stephen","year":"2020","journal-title":"Cytopathology"},{"key":"B25","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/s41389-021-00356-5","article-title":"TRPC1 promotes the genesis and progression of colorectal cancer via activating CaM-mediated PI3K\/AKT signaling axis","volume":"10","author":"Sun","year":"2021","journal-title":"Oncogenesis"},{"key":"B26","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/978-1-4939-7845-8_4","article-title":"Protocols for migration and invasion studies in prostate cancer","volume":"1786","author":"van de Merbel","year":"2018","journal-title":"Methods Molecular Biology Clift. NJ"},{"key":"B27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cellsig.2019.03.004","article-title":"SLFN5 suppresses cancer cell migration and invasion by inhibiting MT1-MMP expression via AKT\/GSK-3\u03b2\/\u03b2-catenin pathway","volume":"59","author":"Wan","year":"2019","journal-title":"Cell. Signalling"},{"key":"B28","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1186\/s12935-022-02496-z","article-title":"SGLT2 inhibition restrains thyroid cancer growth via G1\/S phase transition arrest and apoptosis mediated by DNA damage response signaling pathways","volume":"22","author":"Wang","year":"2022","journal-title":"Cancer Cell Int."},{"key":"B29","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.semradonc.2015.05.007","article-title":"DNA damage response assessments in human tumor samples provide functional biomarkers of radiosensitivity","volume":"25","author":"Willers","year":"2015","journal-title":"Seminars Radiation Oncology"},{"key":"B30","doi-asserted-by":"publisher","first-page":"3197","DOI":"10.7150\/ijms.61944","article-title":"p53-dependent apoptosis is essential for the antitumor effect of paclitaxel response to DNA damage in papillary thyroid carcinoma","volume":"18","author":"Wu","year":"2021","journal-title":"Int. Journal Medical Sciences"},{"key":"B31","doi-asserted-by":"publisher","first-page":"4807","DOI":"10.1038\/s41467-020-18497-3","article-title":"Lymph node metastasis prediction of papillary thyroid carcinoma based on transfer learning radiomics","volume":"11","author":"Yu","year":"2020","journal-title":"Nat. Commun."},{"key":"B32","doi-asserted-by":"publisher","first-page":"3158","DOI":"10.1038\/s41598-017-03230-w","article-title":"TRPC5-induced autophagy promotes drug resistance in breast carcinoma via CaMKK\u03b2\/AMPK\u03b1\/mTOR pathway","volume":"7","author":"Zhang","year":"2017","journal-title":"Sci. Reports"},{"key":"B33","doi-asserted-by":"publisher","first-page":"101172","DOI":"10.1016\/j.tranon.2021.101172","article-title":"Synergic radiosensitization of sinomenine hydrochloride and radioiodine on human papillary thyroid carcinoma cells","volume":"14","author":"Zhao","year":"2021","journal-title":"Transl. Oncology"},{"key":"B34","doi-asserted-by":"publisher","first-page":"3413","DOI":"10.3892\/or.2019.7095","article-title":"TRPC5-induced autophagy promotes the TMZ-resistance of glioma cells via the CAMMK\u03b2\/AMPK\u03b1\/mTOR pathway","volume":"41","author":"Zou","year":"2019","journal-title":"Oncol. Reports"}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1705616\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:29:00Z","timestamp":1771928940000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1705616\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,13]]},"references-count":34,"alternative-id":["10.3389\/fcell.2026.1705616"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2026.1705616","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,13]]},"article-number":"1705616"}}