{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:51:14Z","timestamp":1789804274535,"version":"4.0.1"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>\n                    Wound healing is a complex physiological process involving homeostasis, inflammation, proliferation, migration and tissue remodeling. Impaired keratinocyte migration across the wound bed is a key determinant of non-healing wounds. In this context, plant-derived nanovesicles (PDNVs) have emerged as promising therapeutic agents for wound healing due to their high yield, intrinsic biocompatibility, the ability to traverse biological barriers, and an intrinsic molecular cargo (lipids, proteins, nucleic acids, and phytochemicals) that can exert multitarget effects. In this study, we screened a panel of six PDNVs and found\n                    <jats:italic>Panax notoginseng<\/jats:italic>\n                    -derived PDNVs (PNVs) displayed superior cell proliferation-promoting activity. To further amplify the bioactivity of PNVs, we actively loaded epidermal growth factor (EGF) onto PNVs (EGF@PNVs). By employing LC-MS and miRNA sequencing, we identified abundant small-molecule compounds (e.g., ginsenoside Rb1, Rg1) and miRNAs (e.g., miRNA 159) in PNVs.\n                    <jats:italic>In vitro<\/jats:italic>\n                    experiments demonstrated that PNVs and EGF@PNVs significantly enhanced the proliferation and migration of human keratinocytes (HACAT) as well as the repair of skin mechanical trauma. Moreover, they not only directly accelerated the proliferation and migration of L929 mouse fibroblast cells (L929 cells) but also orchestrated the secretion of TNF-\u03b1 by mouse mononuclear macrophages (RAW264.7 cells). This cytokine subsequently induced the fibroblast activation or phenotype modulation in L929 cells, further augmenting their proliferative and migratory potential. In a mouse skin injury model both formulations accelerated wound closure and exerted immunomodulatory effects, with EGF@PNVs consistently outperforming PNVs. Collectively, our findings introduce EGF@PNVs as a natural, cost-effective, topical alternative to conventional biologics for wound management.\n                  <\/jats:p>","DOI":"10.3389\/fcell.2026.1737435","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T11:54:48Z","timestamp":1773316488000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["EGF-loaded, bioactive-rich Panax notoginseng-derived nanovesicles accelerate skin wound healing"],"prefix":"10.3389","volume":"14","author":[{"given":"Fei","family":"Yu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guofeng","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]},{"name":"Department of Pharmacy, Chengdu Seventh People\u2019s Hospital (Affiliated Cancer Hospital of Chengdu Medical College)","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shenzhen Yiai Health Management Co., LTD","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangsheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Guangdong Pharmaceutical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanxue","family":"Xu","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Chinese Medicine Active Substance Screening and Translational Research, Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-Sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrui","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peilin","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Wingor Biotechnology Co., LTD","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermato-Venereology, The Seventh Affiliated Hospital of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"6455","DOI":"10.7150\/thno.73436","article-title":"Efficacy and safety of small extracellular vesicle interventions in wound healing and skin regeneration: a systematic review and meta-analysis of animal studies","volume":"12","author":"Al-Masawa","year":"2022","journal-title":"Theranostics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1111\/exd.14516","article-title":"Roles of cutaneous cell-cell communication in wound healing outcome: an emphasis on keratinocyte-fibroblast crosstalk","volume":"31","author":"Amiri","year":"2022","journal-title":"Exp. 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