{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T09:05:48Z","timestamp":1779786348115,"version":"3.53.1"},"reference-count":99,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>\n                    Retinoic acid (RA) signalling is essential for vertebrate development, but the spatiotemporal dynamics of its regulation remain largely uncharacterized in specific cellular contexts. Here, we introduce a novel gene trap transgenic reporter line for the zebrafish\n                    <jats:italic>cyp26c1<\/jats:italic>\n                    gene, a key regulator of RA metabolism. Through a screen of a\n                    <jats:italic>gal4<\/jats:italic>\n                    -based gene and enhancer trap collection, we isolated the\n                    <jats:italic>tg(gSAIGFF104A)<\/jats:italic>\n                    line. In this line, a\n                    <jats:italic>gal4<\/jats:italic>\n                    gene trap cassette is inserted into the third intron of the\n                    <jats:italic>cyp26c1<\/jats:italic>\n                    gene, allowing it to faithfully drive reporter expression in a pattern that mirrors the endogenous\n                    <jats:italic>cyp26c1<\/jats:italic>\n                    expression in zebrafish embryos. Using this reporter, we characterized the expression of\n                    <jats:italic>cyp26c1<\/jats:italic>\n                    with cellular resolution, revealing a highly dynamic and localized expression in the developing telencephalon, diencephalon, hindbrain, otic vesicles, pharyngeal arches, and other ectodermal derivatives, as well as in subdomains of the retina. Furthermore, we demonstrate that this line responds to both exogenous and genetic manipulations of RA signalling, particularly within the retina. Our reporter provides a valuable resource for investigating the intricate biology of RA signalling in zebrafish development and disease, offering a tool for tracing and manipulating\n                    <jats:italic>cyp26c1<\/jats:italic>\n                    -expressing cells.\n                  <\/jats:p>","DOI":"10.3389\/fcell.2026.1753548","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T08:40:26Z","timestamp":1779784826000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel cyp26c1-driven reporter line to study boundaries of retinoic acid signalling during zebrafish development"],"prefix":"10.3389","volume":"14","author":[{"given":"Rosana","family":"Reyes-Pinto","sequence":"first","affiliation":[{"name":"Center for Integrative Biology, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Arancibia-Altamirano","sequence":"additional","affiliation":[{"name":"Center for Integrative Biology, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"V\u00e1squez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Center for Integrative Biology, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mat\u00edas","family":"Ben\u00edtez","sequence":"additional","affiliation":[{"name":"Center for Integrative Biology, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aar\u00f3n","family":"Villanueva","sequence":"additional","affiliation":[{"name":"Center for Integrative Biology, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Lahne","sequence":"additional","affiliation":[{"name":"Institute of Ophthalmology, University College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan B.","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Institute of Ophthalmology, University College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Developmental Biology, National Institute of Genetics","place":["Mishima, Japan"]},{"name":"Choju Medical Institute, Fukushimura Hospital","place":["Toyohashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo E.","family":"Valdivia","sequence":"additional","affiliation":[{"name":"Center for Integrative Biology, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]},{"name":"Escuela de Biotecnolog\u00eda, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/j.devcel.2018.04.003","article-title":"Cell identity switching regulated by retinoic acid signaling maintains homogeneous segments in the hindbrain","volume":"45","author":"Addison","year":"2018","journal-title":"Dev. 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