{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:54:38Z","timestamp":1781848478112,"version":"3.54.5"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>We aimed to investigate the association between genes related to disulfidptosis\u2014a form of cell death caused by aberrant disulfide stress and cytoskeletal collapse\u2014and the molecular features of minimal change disease (MCD), the leading cause of primary nephrotic syndrome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Data from the GeneCards and Gene Expression Omnibus (GEO) databases were integrated to systematically analyze disulfidptosis-related genes in MCD. Machine learning approaches\u2014including generalized linear model (GLM), support vector machine (SVM), random forest (RF), and extreme gradient boosting (XGBoost)\u2014pinpointed four hub genes. These genes were used to construct a diagnostic nomogram. Molecular groups were defined by consensus clustering, and pathway alterations were explored through gene set variation analysis and gene set enrichment analyses. Gene expression was validated by immunohistochemistry (IHC) and immunofluorescence (IF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The diagnostic model based on\n                      <jats:italic>FLNC<\/jats:italic>\n                      ,\n                      <jats:italic>GYS1<\/jats:italic>\n                      ,\n                      <jats:italic>INF2<\/jats:italic>\n                      , and\n                      <jats:italic>MYH11<\/jats:italic>\n                      demonstrated exploratory discriminatory performance (AUC = 0.839). Consensus clustering based on the four hub genes suggested two potential molecular groups (C1 and C2), and their differences in pathway enrichment and immune characteristics were explored.\n                      <jats:italic>MYH11<\/jats:italic>\n                      expression was markedly higher in the C2 group and correlated with cytoskeletal remodeling and immune modulation. Experimental validation confirmed significant upregulation of\n                      <jats:italic>MYH11<\/jats:italic>\n                      in MCD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study indicates a potential link between disulfidptosis-related genes and MCD, and presents an exploratory diagnostic and molecular classification system that requires further validation in larger cohorts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1790068","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:46:37Z","timestamp":1781847997000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["An exploratory analysis of disulfidptosis-related gene signatures in minimal change disease identifies metabolic and immune associations"],"prefix":"10.3389","volume":"14","author":[{"given":"Jiahui","family":"Li","sequence":"first","affiliation":[{"name":"First Affiliated Hospital of Gannan Medical University","place":["Ganzhou, China"]},{"name":"Guangdong Engineering Research Center of Low-Carbon Synthetic Biotechnology, School of Biology and Biological Engineering, South China University of Technology","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quhuan","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Research Center of Low-Carbon Synthetic Biotechnology, School of Biology and Biological Engineering, South China University of Technology","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Shen","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaohong","family":"Nie","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital of Gannan Medical University","place":["Ganzhou, China"]},{"name":"The First Clinical Medical College of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1126\/science.aaw9872","article-title":"Cysteine depletion induces pancreatic tumor ferroptosis in mice","volume":"368","author":"Badgley","year":"2020","journal-title":"Science."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e173","DOI":"10.1016\/S1470-2045(14)71116-7","article-title":"Nomograms in oncology: more than meets the eye","volume":"16","author":"Balachandran","year":"2015","journal-title":"Lancet Oncol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"5613","DOI":"10.3390\/ijms25115613","article-title":"Minimal change disease: pathogenetic insights from glomerular proteomics","volume":"25","author":"B\u0103rar","year":"2024","journal-title":"Int. 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