{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:49:12Z","timestamp":1782107352561,"version":"3.54.5"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Di (2-ethylhexyl) phthalate (DEHP), a pervasive environmental pollutant, poses significant health risks, yet its role in stress urinary incontinence (SUI) remains largely unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study integrated network toxicology, molecular docking, and animal experiments to investigate DEHP-induced SUI mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Through cross-analysis of multiple public databases, we identified 95 potential targets linking DEHP exposure to SUI. GO and KEGG enrichment analyses revealed that these targets are primarily involved in immune-related pathways, including apoptosis, TNF signaling, NOD-like receptor signaling, and inflammatory mediator regulation of TRP channels. Using protein interaction network analysis, we further identified five core targets (NFKB1, CASP3, BCL2, TLR4, MMP9). Molecular docking confirmed strong binding affinities between DEHP and these proteins. In vivo experiments demonstrated that DEHP exposure induces urethral sphincter damage and degeneration in rats, thereby accelerating SUI progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study elucidates the molecular mechanisms underlying DEHP-induced SUI and highlights network toxicology as a powerful approach for assessing health risks of environmental contaminants.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1866437","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:38:23Z","timestamp":1782106703000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["DEHP induces stress urinary incontinence by causing urethral sphincter dysfunction via inflammation and apoptosis: a mechanism study based on animal experiments, network toxicology, and molecular docking"],"prefix":"10.3389","volume":"14","author":[{"given":"Xiaozhi","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Urology, Institute of Urology, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, Institute of Urology, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology, Institute of Urology, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Urology, Institute of Urology, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Department of Urology, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Urology, Institute of Urology, West China Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Department of Urology, West China Tianfu Hospital, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"305","DOI":"10.2174\/1871530319666191009154751","article-title":"The expression of hormone receptors as a gateway toward understanding endocrine actions in female pelvic floor muscles","volume":"20","author":"Castel\u00e1n","year":"2020","journal-title":"Endocr. 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