{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:31:14Z","timestamp":1787725874089,"version":"build-2784847793"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Acute stress can disrupt gastrointestinal function. Interstitial cells of Cajal (ICCs) regulate gastric motility; however, the effects underlying their alterations during stress remain unclear. We aimed to explore the potential impact of acute stress on alterations in gastric ICCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Animals were assigned to one control and four study groups (0, 24, and 72\u00a0h). Acute stress was induced via right chest puncture using a Hopkinson bar. Lung injury was assessed using hematoxylin-eosin staining, gastric ICCs were examined using immunohistochemistry, immunohistofluorescence, and TdT-mediated dUTP nick-end labeling assays. Transmission electron microscopy was used to evaluate ultrastructural changes. Western blotting and real-time polymerase chain reaction were used to analyze SCF\/c-kit, protein 53 (p53), and the Wnt\/\u03b2-catenin signaling pathways. Enzyme-linked immunosorbent assay was used to quantify nuclear factor kappa B (NF-\u03baB) p65, interleukin (IL)-1\u03b2, IL-6, IL-9, IL-10, tumor necrosis factor-alpha (TNF-\u03b1), and cortisol. Spectrophotometry was used to determine the levels of reactive oxygen species (ROS) and malondialdehyde (MDA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The lung tissue exhibited congestion, edema, and the destruction of the alveolar structure following puncture. The gastric ICCs tested positive for the surface markers CD117\/c-kit and CD34. The population and apoptosis of the gastric ICCs were altered in the acute stress condition. Moreover, acute stress caused dysregulated SCF\/c-kit, Wnt\/\u03b2-catenin, p53 and NF-\u03baB pathways. IL-1\u03b2, IL-6, IL-9, IL-10, TNF-\u03b1, and cortisol demonstrated altered expression levels, whereas ROS and MDA were upregulated in the acute stress condition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Acute stress damages gastric ICCs through the Wnt\/\u03b2-catenin, JAK\/STAT, and NF-\u03baB signaling pathways, as well as p53-and SCF\/c-kit pathway-mediated abnormalities and oxidative stress, contributing to functional alterations in gastric ICCs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1872384","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:02:22Z","timestamp":1787724142000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of acute stress on gastric interstitial cells of Cajal disorder"],"prefix":"10.3389","volume":"14","author":[{"given":"Zhen-peng","family":"Huang","sequence":"first","affiliation":[{"name":"Faculty of Nursing, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine, Xi\u2019an Medical University","place":["Xi\u2019an, China"]},{"name":"Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University","place":["Xi\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Oncology, Renmin Hospital of Wuhan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-wei","family":"Wan","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2019an Medical University","place":["Xi\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2019an Medical University","place":["Xi\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-wang","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2019an Medical University","place":["Xi\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo-yuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine, Xi\u2019an Medical University","place":["Xi\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"343","DOI":"10.33314\/jnhrc.v19i2.3432","article-title":"Epidemiology of road traffic accidents in Nepal from 2009\/10 to 2019\/20: a 10 year study","volume":"19","author":"Atreya","year":"2021","journal-title":"J. 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